ABSTRACT
Development of multi drug resistant organism has been high due to improper use of antibiotics. That made the necessity to develop new drug molecules. In this study an effort was made to find a new alternative. A wild type microorganism was isolated from soil and was identified as Bacillus and confirmed as Bacillus subtilis species by 16S r RNA sequencing. The strain was identified to have the ability to produce bacteriocin by stab overlay assay. Bacteriocin was produced in nutrient broth and that was extracted by organic solvent extraction using chloroform and further purification was carried out by HPLC and the molecular weight of the bacteriocin was analysed by SDSPAGE. Antimicrobial activity was analysed on four strains Pseudomonas sp, Staphylococcus sp, Klebsiella sp and Proteus sp. and was found to be sensitive towards the analyzed strains.