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Objective:To investigate the relationship between white blood cells, neutrophil-lymphocyte ratio(NLR), platelet-lymphocyte ratio(PLR) and monocyte-lymphocyte ratio(MLR) with patients suffering from first episode depression.Methods:This retrospective study was conducted among inpatients of Hebei General Hospital from January 2021 to December 2021.Ultimately, 193 patients with first-episode depression were enrolled.According to the score of Hamilton depression scale-24 (HAMD-24), the patients were divided into mild-moderate depression group(20≤HAMD-24<35 score, n=98) and severe depression group (HAMD-24 score ≥35, n=95). White blood cells and the counts of each cell subtype were detected and the NLR, MLR and PLR were calculated.SPSS 25.0 statistical software was used to analyze the data.Mann-Whitney U test was used to compare differences in the two groups and Binary Logistic regression analyses were performed to recognize the predictive factors of the severity of first episode depression. Results:(1) The white blood cells and NLR in the severe depression group were significantly higher than those in the mild-moderate depression group (white blood cells: 5.77(2.05)×10 9/L vs 5.11(1.31)×10 9/L; NLR: 1.86 (1.04) vs 1.57(0.55), P<0.05). There were no significant differences in PLR and MLR between the two groups ( P>0.05). (2)Multiple regression analysis of NLR, white blood cells and HAMD-24 score showed that there were significant differences in the effect of different white blood cells and NLR levels on HAMD-24 score( B=1.398, P=0.003; B=2.624, P=0.001). (3)Binary Logistic regression revealed that white blood cell count and NLR were risk factors for the severity of depression patients( OR were 1.612 and 2.336, respectively, P<0.05). Conclusion:The results suggest that white blood cells and NLR may be relate with the severity of first episode depression.
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This study investigated the effect of puerarin on human umbilical vein endothelial cells (HUVEC) injured with hydrogen peroxide (H2O2). HUVEC were divided into three groups: a control group, a model group (H2O2 400 μmol·L-1) and a puerarin-treated group (3, 10, 30 and 100 μmol·L-1). HUVEC were cultured with varied concentration of puerarin for 2 h and treated with H2O2 for another 24 h. Cell proliferation was detected by a CCK-8 assay. The mitochondrial membrane potential was measured by a JC-1 fluorescent probe. A transwell chamber assay was adopted to observe cell migration ability. Mitochondrial respiratory function was measured in a two-chamber titration injection respirometer (Oxygraph-2k). The expression of interleukin-1β (IL-1β), interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) was detected by quantitative real-time PCR. The expression of pyroptosis-mediated proteins, including cleaved-cysteinyl aspartate-specific proteinase-1 (caspase-1), N-gasdermin D (N-GSDMD), NOD-like receptor protein 3 (NLRP3) and purinergic ligand-gated ion channel 7 receptor (P2X7R) was detected by Western blot. The results show that 400 μmol·L-1 H2O2 treatment for 24 h causes obvious damage to HUVEC. Compared with the model group, puerarin protected against cellular injury in a dose-dependent manner, with the greatest effect at a dose of 30 and 100 μmol·L-1. Puerarin significantly decreased the mitochondrial membrane potential and improved mitochondrial function. Puerarin inhibited cell migration induced by H2O2, suppressed the expression of IL-1β, IL-18 and TNF-α, and down-regulated the pyroptosis-mediated protein. These changes are statistically significant (P < 0.05). These findings demonstrate that puerarin has a protective effect against H2O2-induced oxidative damage of HUVEC by inhibiting the migration of HUVEC cells. The mechanism may be related to improved mitochondrial respiratory function and inhibition of pyroptosis.
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Ischemic heart disease (IHD), which has been considered to be exclusively caused by stenosis or occlusion of coronary artery, is a significant cause of morbidity and mortality worldwide. Mitochondrial dysfunction is the main pathological basis of ischemic heart disease and reperfusion injury, and moderate mitochondrial autophagy can selectively remove damage proteins and organelles to maintain intracellular homeostasis, so mitochondrial autophagy is important for maintaining the homeostasis of cardiomyocytes. Natural drugs from plants are widely used in ischemic heart disease. In recent years, more and more natural drugs have been proven to alleviate myocardial cell damage after ischemia/reperfusion through mitochondrial autophagy. This paper reviews the research progress of natural drugs from plants medicines regulating mitochondrial autophagy in the treatment of ischemia heart disease.
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Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton. Methods Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion). Myocardial infarct area was determined by TTC staining. Cardiac function was monitored by a microcatheter. ANP, 15-F2t-isoprostane, nitrotyrosine and MDA were measured by assay kits. Levels of p-Akt, total-Akt and GAPDH were determined by Western Blot. Results Diabetic rats subjected to myocardial IR exhibited severe myocardial infarction and oxidative stress injury, lower APN in the plasma and cardiac p-Akt expression ( P <0.05). IPO significantly attenuated myocardial injury and up-regulated plasma APN content and cardiac p-Akt expression in non-diabetic rats but not in diabetic rats. Linear correlation analysis showed that the expression of adiponectin was positively correlated with p-Akt and negatively correlated with myocardial infarction area ( P <0.01). Conclusion Protective effect of IPO was tightly correlated with the expression of adiponectin, exacerbation of I/R injury and ineffectiveness of IPO was partially due to the decline of adiponectin and inactivation of Akt in diabetes mellitus.
Subject(s)
Animals , Male , Rats , Myocardial Reperfusion Injury/prevention & control , Diabetes Mellitus, Experimental/metabolism , Adiponectin/therapeutic use , Ischemic Postconditioning/methods , Blood Glucose/analysis , Myocardial Reperfusion Injury/metabolism , Rats, Sprague-Dawley , Disease Models, AnimalABSTRACT
<p><b>OBJECTIVE</b>To study the fingerprint of Zedoary Turmeric Oil (ZTO) as the bulk drug of Kingkong Elemene for making it safe, effective, stable, and controllable.</p><p><b>METHODS</b>Fingerprints were detected by gas chromatography. β-elemene peak was regarded as reference peak (S). The relative peak area of each common peak and the relative retention time were calculated. With a total of modes for reference, the fingerprints of 10 batches of Kingkong ZTO were detected, and their similarity was calculated by traditional Chinese medicine (TCM) fingerprint similarity calculation software.</p><p><b>RESULTS</b>The determination method was stable and reliable. Totally 19 common characteristic peaks of Kingkong ZTO was found. The fingerprint similarity of these batches of Kingkong ZTO were not lower than 0.96.</p><p><b>CONCLUSIONS</b>Gas chromatography for detecting the fingerprint of Kingkong ZTO was reliable and repeatable. The established fingerprint of Kingkong ZTO could guarantee the quality stability and safety of different product batches.</p>
Subject(s)
Chromatography, Gas , Curcuma , Chemistry , Drugs, Chinese Herbal , Chemistry , Plant Oils , Chemistry , Sesquiterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>This study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.</p><p><b>METHOD</b>The HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.8 μm) with 0.1% fomic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 · mL(-1) · min(-1); The detective wavelength is 325 nm; The column temperature is 45 °C.</p><p><b>RESULT</b>The results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95.</p><p><b>CONCLUSION</b>This method is able to be a scientific basis of quality assessment according to its convenient and reliable.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Inula , ChemistryABSTRACT
OBJECTIVE: To investigate the therapeutic efficacy of treatment of Bacillus licheniformis (BL) on ulcerative colitis(UC) in BALB/c mice. METHODS: The therapeutic effect of different treatment was evaluated in seven acute UC mice groups and one control group, including the normal group, the model group, the solvent group, the sulfasalazine (SASP) treated group, the high dose BL treated group, the low dose BL treated group, the high dose BL with SASP treated group and the low dose BL with SASP treated group. The disease activity index (DAI) score of mice were recorded every other day since the experiment started. On the 15th day, all the mice were killed, then gross injury score in colonic mucosa and histopathological changes were measured. The expression levels of NF-κB and Bcl-2 in mouse colonic mucosa was also determined by immunohistochemistry method. RESULTS: The DAI score, the colonic gross injury score, the histopathological changes and the expression level of NF-κB and Bcl-2 of mice colonic mucosa in drug treated groups were decreased significantly when comparing with the drug untreated groups. Moreover, these results were even better in two drugs combined treated groups than in single drug treated groups (P<0.05). CONCLUSION: BL has the efficacy of remission DSS-induced acute ulcerative colitis in mice, and there is more effective when it is administered with SASP. The results indicat that the therapeutic effort is via inhibiting the expression of NF-κB and Bcl-2 in colonic mucosa.
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To evaluate the prognostic value of late gadolinium enhancement [LGE] in dilated cardiomyopathy [DCM] patients. We searched PubMed, MEDLINE, the Cochrane library and EMBASE databases from September to December 2012 in the Renmin Hospital of Wuhan University, Wuhan, China for studies of LGE in DCM patients. We extracted the clinical outcomes [all-cause mortality, cardiovascular mortality, sudden cardiac death [SCD], aborted SCD, heart failure hospitalization] after carefully reviewed. A meta-analysis was performed to calculate pooled odds ratios [OR] with 95% confidence intervals [CIs] for prognostic outcomes in LGE positive versus LGE negative patients with DCM. Five studies for 545 DCM patients were contained in this meta-analysis. The results showed LGE positive patients was significantly associated with higher cardiovascular mortality [pooled OR: 2.67; 95% CI: 1.12-6.35; p=0.03], aborted SCD [pooled OR: 5.26; 95% CI: 1.57-17.55; p=0.007], and heart failure hospitalization [pooled OR: 3.91; 95% CI: 1.99-7.69; p<0.001]. Late gadolinium enhancement during cardiac MRI is significantly associated with cardiovascular mortality, aborted SCD and heart failure hospitalization in DCM patients. The LGE can be a potential stratification tool to predict the risk of cardiac events among patients with DCM
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Objective To observe the clinical effect of oral administration of Fufangejiao liquid after drug-induced abortion. Methods A total of 120 women within 7 weeks' of gestation, who needed to terminate pregnancy and asked for drug-induced abortion, were evenly randomized into two groups: experimental group and control group. The experimental group was given Fufangejiao liquid (20ml, tid, for 7 days) after the drug-induced abortion; the control group was given Chanfukang particles (lOg, tid, for 7 days) after drug-induced abortion. The drug-induced abortion and amount of bleeding were observed in the two groups. Results (1) The complete abortion rate was 90. 0%(54 cases) in the experimental group and 86. 7% (52 cases) in the control group. (2) There were 88. 9%(48/ 54 cases) of the patients in the experimental group and 76. 9% (40/52) in the control group had a similar bleeding amounts to the usual menstrual amount. There were 9. 3%(5 cases) of the patients in the experimental group and 19. 2%(10 cases) in the control group had more bleeding (≤1 fold) than the usual menstrual amount(P<0. 05). (3)The days of bleeding, bleeding amount, and menstrual onset were similar in the two groups. Conclusion Administration of Fufangejiao liquid after drug-induced abortion can reduce the amount of bleeding after abortion and is therefore worth popularizing.
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<p><b>OBJECTIVE</b>To investigate the effect of compound Danshen Droplet-pill (DS) combined with trimetazidine (TMZ) in treating senile unstable angina pectoris (SUAP).</p><p><b>METHODS</b>One hundred and twenty patients with SUAP were eaually and randomly divided into 2 groups, the treated group and the control group. Changes of angina, occurrence of arrhythmia, myocardial infarction and sudden death, myocardial ischemia in ECG and partial indexes of heart function were observed.</p><p><b>RESULTS</b>The total effective rate in the treated group was 78.3%, while that in the control group was 53.3%, comparison of the two groups showed significant difference (P < 0.05). The incidence rate of arrhythmia in the two groups was 18.2% and 30.0% respectively and that of acute myocardial infarction and sudden death was 0 and 5.0% respectively, also showed significant difference between them (P < 0.05). Condition of myocardial ischemia revealed in ECG and partial indexes of heart function in the treated group were all improved to certain extent. Conclusion DS combined with TMZ is superior in treating SUAP.</p>