miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice / 神经科学通报·英文版

Post-stroke depression (PSD) is a serious and common complication of stroke, which seriously affects the rehabilitation of stroke patients. To date, the pathogenesis of PSD is unclear and effective treatments remain unavailable. Here, we established a mouse model of PSD through photothrombosis-induced focal ischemia. By using a combination of brain imaging, transcriptome sequencing, and bioinformatics analysis, we found that the hippocampus of PSD mice had a significantly lower metabolic level than other brain regions. RNA sequencing revealed a significant reduction of miR34b-3p, which was expressed in hippocampal neurons and inhibited the translation of eukaryotic translation initiation factor 4E (eIF4E). Furthermore, silencing eIF4E inactivated microglia, inhibited neuroinflammation, and abolished the depression-like behaviors in PSD mice. Together, our data demonstrated that insufficient miR34b-3p after stroke cannot inhibit eIF4E translation, which causes PSD by the activation of microglia in the hippocampus. Therefore, miR34b-3p and eIF4E may serve as potential therapeutic targets for the treatment of PSD.

Based on the Hippo signaling pathway to explore the mechanism of autophagy in lung injury of acute respiratory distress syndrome induced by sepsis / 中华危重病急救医学

The systemic inflammatory response caused by various pathogenic factors is a key stage in the development of acute respiratory distress syndrome (ARDS). At present, suppression of the inflammatory response and symptomatic support are main methods for the treatment of ARDS. Alveolar epithelial autophagy has an important role in the regulation of the inflammatory response in ARDS. Autophagy is a normal immune mechanism in the body, and it is a metabolic process by which phagocytes degrade intracellular components with the help of lysosomes to maintain intracellular homeostasis. Current studies have shown that pathogenic factors both inside and outside the lung can cause alveolar epithelial cells to form an unfavorable internal environment of hypoxia, starvation, infection, and even apoptosis by triggering inflammatory responses, leading to autophagy dysfunction. Excessive autophagy activation can continue to aggravate inflammatory responses. Autophagy related proteins such as Beclin1, microtubule-associated protein 1 light chain 3 (LC3), mammalian target of rapamycin (mTOR), and p62 are common autophagic markers in current research, which play a crucial role in regulating the autophagic process and the development of lung injury. Therefore, the expression of cellular autophagy genes can be used as early markers and important mechanisms of lung injury in septic ARDS. The Hippo signaling pathway is derived from the protein kinase Hippo in Drosophila, and the Hippo and autophagy are two conserved pathways that are essential for the protection of homeostasis in vivo. The mutual regulation of Hippo signaling pathway and autophagy is currently a hot topic in the academic community. This paper reviews the relevant literature to explore whether the Hippo signaling pathway can regulate cellular autophagy to alleviate the inflammatory response in septic ARDS, so as to provide further research directions for the treatment of ARDS.

Effect of Soluble CD40L on Leukemia K562 Cells and Its Mechanism / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the effect of sCD40L on biological behavior of leukemia cell line K562 and the possible mechanism.</p><p><b>METHODS</b>The different concentration of sCD40L was used to treat K562 cells, and the optimum concentration of sCD40L was screened by detecting the proliferation inhibition rate of K562 cells. The optimum concentration of sCD40L was used to treat K562 cells, the cell apoptosis rate and expression level of P53 and BCL-2 were detected by flow cytometry and the expression levels of Caspase 8 and Caspase 3 were detected by ELISA.</p><p><b>RESULTS</b>The optimum concentration of sCD40L was 4 µg/ml. After treated with sCD40L, the cell apoptosis rate, the expression of apoptosis-related factor P53 and the expression of Caspase 8 and Caspase 3 were significantly up-regulated in K562 cells,but the expression of BCL-2 was significantly down-regulated.</p><p><b>CONCLUSION</b>4 µg/ml sCD40L can inhibit the cell proliferation and promote the apoptosis of K562 cells, its mechanism may be related with mitochondrial and P53 pathway.</p>

Effect of electroacupuncture on the cognitive function and plasma antibodies against beta-amyloid protein in aged rats with ketamine anesthesia / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effects of electroacupuncture (EA) on the changes of behavior after ketamine anesthesia, and changes of serum antibodies against beta-amyloid (Abeta) and Abeta protein in the hippocampus of aged rats, thus exploring the effects of EA on the cognitive dysfunction.</p><p><b>METHODS</b>Thirty 14-month old SD rats were randomly divided into 3 groups, i. e. , the control group (Group A), the ketamine anesthesia group (Group B), and the EA+ketamine anesthesia group (Group C), 10 in each group. 50 mg/kg katemine was intraperitoneally injected to rats in Group B and Group C, once daily for 7 successive days. EA was performed to rats in Group C from the 1st day of the experiment after rats awoke completely from anesthesia, twice daily for 7 successive days. Changes of the ratio of the swim time in the original platform quadrant to the total swim time and the escape latency phase were observed by Morris water maze. The peripheral blood was withdrawn by the end of the experiment. Serum anti-Abeta antibody contents were detected using enzyme-linked immunosorbent assay (ELISA). The expressions of Abeta in the hippocampus were detected using Westen blot.</p><p><b>RESULTS</b>Long-term application of ketamine could lower aged rats' cognitive function. In the navigation test, the escape latency phase of rats in Group B was significantly prolonged ( P < 0.01) . On the 7th day of the experiment, the serum level of anti-Abeta antibodies was lower in Group B than in Group A (P < 0.05), while the serum level of anti-Abeta antibodies was significantly higher in Group C than in Group B (P < 0.01). On the 7th day of the experiment, the expression of Abeta in the hippocampus was higher in Group B than in Group A (P < 0.05).</p><p><b>CONCLUSION</b>EA could increase the contents of anti-Abeta antibodies in aged rats with ketamine anesthesia, decrease the expression of Abeta in the hippocampus, alleviate the deposition of Abeta, thus improving rats' cognitive dysfunction.</p>

Bioactivity diversity and functional mechanism of tetrahydroisoquinoline alkaloids / 药学学报

Tetrahydroisoquinoline alkaloids distributed widely in the nature and some have a broad application in clinic. More attention has been paid in recent years on this type of alkaloid, owing to the diverse range of biological activities exhibited by these alkaloids and the discovery of new functional mechanisms and molecular targets underlying these activities. This article summarized the recent advances in the biological activities and functional mechanism of tetrahydroisoquinoline, which included the activities such as antitumor, antibiotic, antivirus, anti-inflammatory, anticoagulation, bronchodilation, and the action on central nervous system, with the purpose of providing some ideas in the study of biological activity of this type of alkaloid and in the search for lead-compound and rational drug design.

Detection of hepatotoxic pyrrolizidine alkaloids in Ligularia Cass. with LC/MSn / 药学学报

<p><b>AIM</b>To detect the hepatotoxic pyrrolizidine alkaloids (HPA) in the genus Ligularia Cass..</p><p><b>METHODS</b>The alkaloid extracts of Ligularia plant materials were detected and analyzed by the method of combination of TLC, and LC/MSn.</p><p><b>RESULTS</b>Among 22 species of Ligularia Cass., HPA were detected in 18 species with LC/MSn, and no HPA was detected in the remaining 4 species.</p><p><b>CONCLUSION</b>HPA was first detected with LC/MSn in L. tongelensis and other 15 species of Ligularia Cass.; HPA from these plants should be isolated, separated and identified and it is necessary to study the activities and toxicities of the HPA. The types and kinds of HPA from different species and sources are different, they should be detected separately.</p>

Resource investigation and quality evaluation on wild Corydalis yanhusuo / 中国中药杂志

<p><b>OBJECTIVE</b>To study the resource and the quality of wild Corydalis yanhusuo distributed in China.</p><p><b>METHOD</b>Distribution was observed and samples of wild C. yanhusuo were collected and qualities were evaluated by determining six main alkaloids contained in the samples.</p><p><b>RESULT</b>The main distribution of wild Corydalis yanhusuo was in the hills around middle and lower reaches of Yangtze River. Its distributive areas were continuously decreasing. The alkaloids contents in the samples varied among different populations.</p><p><b>CONCLUSION</b>The alkaloids contents in wild populations of C. yanhusuo are diverse. The main kinds of alkaloids in some wild populations are higher than cultivated ones, which are valuable for breeding. The wild C. yanhusuo propagate well, however they are endangered due to environment problem, and should be protected.</p>