ABSTRACT
In the present study, 20 fungal strains were isolated from tomato rhizosphere of Senegal. Of 20 strains, five showedthe chitinolytic activity on chitin agar medium. Of the five strains, NG4 showed the maximum solubilization zone.This strain was identified by preliminary biochemical and 18S rRNA sequencing analysis. Enzyme productionstarted after 3 days of incubation and maximum was observed after 5 days of incubation. Culture filtrate amendedwith 0.1% colloidal chitin was used in the production medium. The optimum conditions for maximum chitinaseactivity are – 6 days of growth and temperature of 30°C at pH 6.0. The chitinase activity was also influenced by theaddition of carbon and nitrogen sources in the production medium.
ABSTRACT
Objective To isolate and identify the compounds in the essential oils from the leaves of Callistemon viminalis (D.R.) and Melaleuca leucadendron (Linn.) collected in Dakar, Senegal. Methods The essential oils from the leaves of these two myrtaceaes were extracted by steam distillation and analyzed by gas chromatograph and gas chromatography–mass spectrometer. Results A total of 34 constituents were identified in the oil of Callistemon viminalis and the major compounds were 1.8-cineole (58.12%), limonene (9.72%), α-terpineol (9.56%), geranial (6.02%), δ-elemene (3.53%), myrcene (2.96%) and α-pinene (2.49%). For the essential oil of Melaleuca leucadendron, 43 constituents were identified, and 1.8-cineole (28.87%), epiglobulol (23.06%), α-pinene (12.22%), limonene (11.65%) and α-terpineol (7.06%) were major compounds. Conclusions Considering properties of the identified major compounds, essential oils of both studied myrtaceae could be used in the medicine field including the food, pharmaceutical and cosmetic industry.