ABSTRACT
Objective; To explore the possible effect of hyperthermia combined with Prog on a human tongue cancer cell line Tca8113 and drug-resistance cell line Tca8113/BLM in vitro. Methods;The effect of hyperthermia combined with Prog on Tca8113 and Tca8113/ BLM cells were determined by MTT. The concentration of ADM, the expression of P-gp and MRP were detected by flow cytometry (FCM). Results;Compared with Prog or hyperthermia, the hyperthermia at 41 X. for 1 h combined with Prog showed obvious inhibitory effect in Tca8113 and Tca8113/BLM cells(P<0.01). The concentration of ADM in Tca8113 and Tca8113/BLM cells increased obvi-ously(P<0.01). Expression levels of P-gp and MRP in Tca8113 and Tca8113/BLM cells decreased significantly. Conclusion:The hyperthermia combined with Prog can increase the intracellular concentration of ADM in Tca8113 and Tca8113 /BLM cells, which reverse the drug-resistance of Tca8113/BLM cells to BLM and down-regulate expressions of P-gp and MRP.