ABSTRACT
The effect of different concentrations [10 microg, 25 microg, 50 microg/ml] of crude snake venom on protein contents of different normal tissues of rabbit was studied. Our objective was to determine and compare the effectiveness of snake venom [SV] on normal tissues before its use in vivo for the treatment of different diseases, especially cancers. Surgical portions of normal tissues of liver, heart, and kidney were removed from rabbits and homogenized. All homogenate samples were incubated with and without SV for 30 minutes at 37°C and measured with the spectrophotometer [Spectron 21]. The in vitro effect of cobra snake venom on total proteins was quantitatively evaluated in different normal rabbit tissues. The different concentrations of SV i.e. 10 microg, 25 microg, and 50 microg/ml demonstrated a significant linear increase in protein concentrations against controls. Significant and linear increases in protein concentrations suggest multiple sites/receptors for venom to act and disintegrate the macromolecules of proteins. The increase in the numbers of small proteins/peptides provides evidence for the venom-specific actions. A concentration of 10 micro g/ml could be of therapeutic potential