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Objective:To observe the efficacy of combining transcranial direct current stimulation (tDCS) and functional electrical stimulation (FES) therapy on the upper limb function of chronic stroke patients.Method:Three subjects with chronic stroke patients received active tDCS + FES therapy for 4 weeks after 4 weeks of baseline observation.Surface electromyography (sEMG),transcranial magnetic stimulation (TMS) and upper limb part of the Fugl-Meyer assessment (U-FMA) were used to assess the performance of the subjects pre-and-post the intervention.Result:U-FMA scores improved after the intervention.There are five of the seven tested active muscles showed an increasing change in surface electromyography activation levels.Portion of the short latency cortical inhibition and facilitation data of the contralateral hemisphere tested with TMS paired pulse stimulation showed a change post the intervention.Conclusion:Subjects showed improvement with their upper limb motor function,thus the tDCS combine FES therapy may be a promising rehabilitation method for upper limb motor function of stroke in chronic stages.
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Objective To investigate the clinical detection of bladder vascular resistance index in female overactive bladder patients. Methods Bladder vascular resistance index in 40 female overactive bladder patients and 20 healthy controls was measured by transabdominal pulsed wave spectral Doppler imaging. Results Significant difference between female overactive bladder patients and healthy women controls was observed in the resistive index(0.81 ± 0.06 vs.0.64 ± 0.06,P<0.05). Conclusions The results indicate that bladder ischemia may play a role in female overactive bladder patients.
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Objective:To observe the efficacy of combining transcranial direct current stimulation (tDCS) and functional electrical stimulation (FES) therapy on the upper limb function of chronic stroke patients.Method:Three subjects with chronic stroke patients received active tDCS + FES therapy for 4 weeks after 4 weeks of baseline observation.Surface electromyography (sEMG),transcranial magnetic stimulation (TMS) and upper limb part of the Fugl-Meyer assessment (U-FMA) were used to assess the performance of the subjects pre-and-post the intervention.Result:U-FMA scores improved after the intervention.There are five of the seven tested active muscles showed an increasing change in surface electromyography activation levels.Portion of the short latency cortical inhibition and facilitation data of the contralateral hemisphere tested with TMS paired pulse stimulation showed a change post the intervention.Conclusion:Subjects showed improvement with their upper limb motor function,thus the tDCS combine FES therapy may be a promising rehabilitation method for upper limb motor function of stroke in chronic stages.
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Objective To construct and identify a novel IL-10 delivery system by transforming a hIL-10-containing plasmid into B.longum (BL -hIL -10).Methods A plasmid vector pBADs -GFP was selected which had been built by previous test and biosynthetic hIL-10 plasmid,through double enzyme digestion and enzyme reaction,to construct and identify PBADs-hIL-10 shuttle plasmid,then to synthesis BL-hIL-10.hIL-10 was expressed and secreted into the culture supernatant of BL-hIL-10 after 0.2% L-arabinose induction in vitro as examined by Western blot,enzyme-linked immunosorbent assay (ELISA)and RT-PCR;Culture supernatants and bacterium pellets were collected after continuous culture for 12,24 and 36h,respectively.hIL-10 was expressed and secreted into the culture supernatant of BL-hIL-10 after 0.2% L-arabinose induction in vitro as examined by Western blot,enzyme -linked immunosorbent assay (ELISA)and RT -PCR;Culture supernatants and bacterium pellets were collected after continuous culture for 12,24 and 36h,respectively.Results The BL-hIL-10 bacterial strain that can stably express hIL-10 factor was successfully screened out,and the levels of hIL-10 in both superna-tant and cell pellet were similarly reached maximum at 24h of culture.Conclusion BL-hIL-10 as a novel oral hIL-10 delivery system has been successfully established,which established a basis for the treatment of IBS with transgenic Bifidobacterium.
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An automatic control system was designed to suppress pathological tremor on wrist joint with two degrees of freedom (DoF) using functional electrical stimulation (FES). The tremor occurring in the wrist flexion-extension and adduction-abduction was expected to be suppressed. A musculoskeletal model of wrist joint was developed to serve as the control plant, which covered four main muscles (extensor carpi radialis longus, extensor carpi ulnaris, flexor carpi radialis, and flexor carpi ulnaris). A second-order mechanical impedance model was used to describe the wrist skeletal dynamics. The core work was to design the controller and a hybrid control strategy was proposed, which combined inverse model based on feed forward control and linear quadratic regulator (LQR) optimal control. Performance of the system was tested under different input conditions (step signal, sinusoidal signal, and real data of a patient)., The results indicated that the proposed hybrid controller could attenuate over 94% of the tremor amplitude on multi-DoF wrist joint.
Subject(s)
Humans , Electric Stimulation , Muscle, Skeletal , Tremor , Therapeutics , Wrist , Wrist JointABSTRACT
This paper demonstrates a 3D rotatory chair and the development of control rotatory system based on PLC, corresponding software and user interface. The PLC-based three-dimensional rotatory chair has the advantage of maneuverability and stability according to the result of the experiment.
Subject(s)
Equipment Design , Labyrinth Diseases , Rehabilitation , Rehabilitation , Software , User-Computer InterfaceABSTRACT
@#This paper reviewed the research of functional electrical stimulation (FES) in China, including mainland, Taiwan and Hong Kong, based on the literature. Specially, the research and development on FES system and device was discussed.
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Objective To perform a DNA-based prenatal diagnosis in a family with recessive dys-trophic epidermolysis bullosa, and to develop a strategy to eliminate matemal cell contamination in arnniotic fluid samples. Methods Amniocentesis was carried out at gestation week 16, amniotic fluid culture was used to separate fetal cells from maternal blood cells. Peripheral blood was obtained from the proband, and her parents. Genomic DNA was extracted from peripheral blood and aminotic cells. Subsequently, PCR and direct sequencing were performed to detect pathogenic mutations in the COL7A1 gone. Karyotype analysis was used to confirm paternal information in amniotic fluid. Linkage analysis between micro-satellite markers was performed to confirm the fetal genotype. Resulta Centrifugation showed visible contamination of aminotic cells by blood cells. Direct sequencing revealed that the proband was a carrier of both maternal mutation, R525X in exon 12, and paternal mutation, R2610X in exon 105, while the fetus only carried the maternal mutation, R525X. The second direct sequencing and hapiotype analysis after elimination of mater-nal blood cells by amniotic fluid culture confirmed that the fetus was a carrier of maternal mutation with nor-real phenotype. The pregnancy continued and a clinically unaffected girl was born at gestation week 40.Conclusion The accuracy of DNA-based prenatal diagnosis could be improved by the combination of direct sequencing, amniotic fluid culture, karyotype analysis and linkage analysis, etc.
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Objective To investigate the diagnostic value of laparoendoscopic exploration and peri-toneum biopsy in patients with ascites of unknown origin and to study the feasibility of replacing hard laparo-endoscope with ultrathin gastroendoscope. Methods A total of 35 patients with ascites of unknown origin underwent laparoendoscopic exploration and biopsies were taken at suspected sites on peritoneum. Results Definite diagnosis was made in 33 patients after laparoendoscopy (94. 3% ), among which there were 10 tuberculosis peritonitis (28. 6% ), 7 metastatic peritoneal carcinoma (20%), 5 liver cancer, 3 liv-er cirrhosis, 3 primary peritoneal carcinoma, 2 ovary cancer, 1 hepatovascular sarcoma, 1 spleen lymphoma and 1 acidophilic enteritis. No procedure related complications were observed. Conclusion Laparoendosco-py is safe and effective in target peritoneal biopsy, and is of great importance in diagnosis of ascites of un-known origin.
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AIM: To observe the effects of herba houttuyniae injection, a chinese medicine, on myocardial injury induced by endotoxin and to explore the possible mechanism. METHODS: 24 male SD rats,weghting (250?30) g, were randomly divided into three groups: normal control group(NC, n=6), endotoxin group (ET, n=6), herba houttuyniae injection puls endotoxin group (HHI+ET, n=6) and herba houttuyniae injection group (HHI, n=6). Heart was isolated from rats and perfused on Langendorff apparatus with Krebs-Henseleit (KH) buffer (Saturation 95%+5%CO_2) at a constant pressure (8.33 kPa) and temperture(37℃). The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) content of efflux from coronary artery were measured at certain time points (0 min, 20 min, 50 min, 80 min). The ventricular function, the concentration of nitric oxide (NO) and the activity of succinate dehydrogenase (SDH) in myocardial tissue were also observed. RESULTS: Compared with ET group, the percentage recovery of the product of left ventricular pressure difference and heart rate [(LVSP-LVEDP)?HR], LVDP,+dp/dt_(max) were all increased apparently in HHI treatment group. The activities of SOD, SDH and the concentration of MDA, NO in HHI treatment group were close to that in normal control group. CONCLUSION: Herba houttuyniae injection protects myocardium against injury induced by endotoxin. It may be related to decrease in the level of NO, the strength of antioxidation, the stability of enzyme activities and the improvement of energy metabolism in myocardium.