ABSTRACT
Tonifying herbs and evil expelling herbs of traditional Chinese medicine (TCM) for chronic kidney diseases in molecular level were studied by computer aided drug design, including analysis of molecular similarity, predictive ADME simulation and predictive toxic simulation. It was found that this technology could distinguish the structure diversity of compounds from TCM, and screen the lead compounds rapidly.
Subject(s)
Humans , Chronic Disease , Therapeutics , Drugs, Chinese Herbal , Chemistry , Pharmacology , Kidney Diseases , Drug TherapyABSTRACT
OBJECTIVE:To establish an HPLC method for determination of protocatechuic acid in Pterospermum heterophyllum.METHODS: The separation was performed on Agilent TC C18(250 mm?4.6 mm,5.0 ?m)column.Moblie phase consisted of acetonitrile-0.1% glacial acetic acid solution(pH 3.16,10 ∶ 90)with flow rate of 1.0 mL?min-1 and detection wavelength of 260 nm.The column temperature was set at 35℃ and injection volume was 10 ?L.RESULTS:The linear range of protocatechuic acid was 0.007 76～0.310 4 ?g(r=0.999 7) with an average recovery of 97.68% (RSD=1.51%,n=6).CONCLUSION:The method is simple,accurate and reproducible for the quality control of P.heterophyllum.
ABSTRACT
OBJECTIVE:To establish the quality standard of Dibiling.METHODS:Rhizoma Coptidis in the formulation was identified qualitatively by TLC,and the content of ephedrine hydrochloride was determined by RP - HPLC.RESULTS: The TLC spots of Rhizoma Coptidis were clear and distinctive.The linear range of ephedrine hydrochloride was 0.088～3.520?g(r = 0.996 0) with an average recovery rate of 100.82%(RSD = 1.19%,n = 6).CONCLUSION:The established standard is applicable for the quality control of Dibiling.