ABSTRACT
Objective: To study the optimum extracting factors of Bushenjiangu Capsule. Methods: Uniform Design was used to arrange experiments, Ursolic acid content was used to evaluate the factor levels, and the optimum extracting factors were determined according experiments and realities. The orthogonal design was used to study three factors including crude drug size extracting time and amount of water. water extracted rate and alcohol extracted substance were used to evaluate the result. Results: The optimum alcohol-extracting factors were 8 times of 70% alcohol, percatating 48 hours. The optimum water-extracting factors were 1cm or smaller the crude drug size, boiling 3 times: 3h,2h,1h, each time, the amount of water was 10,8,6 times. Conclusion: According the optimum extracting factors, the effective substance can be extracted abundantly.
ABSTRACT
AIM:To study the purified factors of Guanxinning for Injection(Radix et Rhizoma salviae miltiorrhizae,Rhizoma Chuanxiong,etc). METHODS: The total of salvianolic acid B、protocatchuci aldehyde and ferulic acid were used to evaluate for the study of type of macroporous adsorption resin,sample volume,the ratio of diameter to height,pH value and sample consistency etc. RESULTS: pH value Guanxinning extract was adjusted to 3.0,through D101 resin finally reached the ratio of diameter to height 1∶2,4 times of water was applied to elution and then 3 times of 70% ethanol for re-elution and collected. CONCLUSION: These factors are approprite for purifying Guanxinning for Injection.