ABSTRACT
Place and Duration: Laboratory of Biochemical Pharmacodynamics, Faculty of Biosciences, University Félix Houphouët - Boigny, from March to June 2018. Methodology: Serial extraction was done using methanol and water as solvents. The study was evaluated by orally daily doses of extracts 300, 600 and 1200 mg/kg. The treatment for 28 days concerned seven groups of animals, the control group and six treated groups. Each group included six animals, 3 males and 3 females. Animals of control group didn’t have any treatment. Animals were observed for general behavioural and signs of abnormalities during the experiment. After all treatments, blood was collected for haematologi cal and biochemical analysis. Liver, kidney, and heart were removed, weighed for histological study. Results: The results showed that, there were not any significant (p ?0.05) changes in both the absolute and relative organ weights between the control and the test groups. Biochemical parameters were statistically equal in all groups. In addition, both extracts did not induce any significant effect on RBC and indices relating to it (HGB, PCV, MCV, MCH and MCHC) throughout the experimental period. But, there was a decrease ( 16.33±1.68) on WBC with methanolic extract compared to control ( 13.79±2.73 ). Histological exam ination of the liver, kidneys, and the heart showed normal organisation and structure of heart, kidneys and liver. Conclusion: It appears that the methanolic and aqueous extracts of hull of Arachis hypogaea did not produce any toxicity in oral subacute toxicity study. However, further studies are needed to confirm long term toxicities.
ABSTRACT
Aims: Birth weight is a powerful predictor of infant growth and survival and depends on the fetal growth environment, which is influenced by maternal nutritional status. However, the association between maternal anthropometric and nutritional factors and birth weight is not well characterized in Côte d'Ivoire. The objective of this study was to determine the maternal anthropometric and nutritional characteristics associated with birth weight. Study Design: This was a retrospective study. Place and Duration of Study: This study was done in maternity hospitals of three municipalities in Abidjan, Côte d'Ivoire, from 1st October to 30 November 2018. Methodology: It consisted in collecting birth data from 146 newborns born from a monofetal pregnancy, whose mothers aged 20 to 42 had participated in a previous survey. Also, the relationship between birth weight, maternal anthropometry and maternal nutrition factors has been studied. Univariate, bivariate and multivariate data analysis was done using SPSS version 25 software. Results: The results indicate mean birth weight of 3118.48±515.39 g and 7.6% and 5.5% respectively of low and excessive birth weight. In a multivariate linear regression, the mean birth weight of newborns of women with medium and high dietary diversity score was higher than those newborn of women with low dietary diversity score (AOR=0.386, 95% confidence interval (CI): 0.072-0.699; p=0.017 and AOR=0.233, 95% CI: 0.016-0.450; p=0.036). Similarly, women with gestational weight gain greater than 7 kg and high stature (>1.55 cm) gave birth to heavier children (AOR=0.551, 95% CI: 0.346-0.756; p=0.000 and AOR=0.633, 95% CI: 0.207-1.059; p=0.004, respectively). Conclusion: Although it revealed the presence of low and excess birth weight, this study has shown that maternal anthropometry and dietary diversity score were associated with birth weight of the baby.
ABSTRACT
This study was conducted to assess the effect of the ethyl acetate fraction (AcEF1) obtained from the 96% extract of Morinda morindoides extract on cardiac tissue integrity and function in rabbits. Methodology: The rabbits were divided in five groups of 6 rabbits each. Groups 2,3,4 and 5 received intraperitoneally, twice a week during four weeks, the ethyl acetate fraction at doses going from 25 to 100 μg/kg body weight while Group 1, received 1 mL of Mac Ewen fluid. Blood sampling was carried out to evaluate aspartate aminotransferase (AST), alanine aminotransferase (ALT), Creatine phosphokinase (CPK), Lactate dehydrogenase (LDH) activities and level of calcium, sodium, potassium, magnesium and chloride in serum. The eighth week of the experiments, histopathological studies were also conducted on rabbits heart. Results: Analysis of the serum markers showed slight increases in AST, ALT, CPK activities as well as sodium, calcium and potassium concentrations (p<0.05). LDH activity, magnesium and chloride concentrations were unchanged compared to their initial values. Histopathological studies had not revealed damages in the structure of the heart. Conclusion: The ethyl acetate fraction did not exert any noxious effect on cardiac tissues and should be no toxic for cardiac tissues.
ABSTRACT
Heliotropium indicum (Boraginaceae) is used in the traditional Ivory Coast pharmacopeia to treat asthma. In the present study, wound healing effect of n- butanol fractions was evaluated in H292- cells. Fractions which possessed better wound healing activity were fractionated on Sephadex LH20 column chromatography. Two compounds have been isolated which were responsible for this wound healing effect. Their structures were established as Pestalamide B (1) and Glycinamide,N-(1-oxooctadecyl)glycyl-L-alanylglycyl-L-histidyl (2) on the basis of spectral analysis. Both compound 1 and 2 presented wound healing effect compared with the control (P<0.05).