Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack

Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model.

Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack

Objective:To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia.Methods:The in vitro anti-inflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.The level of NO produced in the culture media was determined by Griess method.The iNOS and COX-2 protein expressions were analyzed by Western blot.The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model.Mice mortality was monitored for 5 days after injection of LPS.The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques.Results:The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vio models.The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions.In the septic shock model,ELA dose-dependently protected mice from LPS-induced mortality.Further study on the isolated components of ELA indicated that 9,1 0-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract.Conclusions:These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS,and COX-2 and protects mice from LPS-induced mortality in septic shock model.

Evaluating laparoscopy in Institute of Protection of Mother and Newborn from 1996 to 1999

A retrospective study was performed in 1,142 cases experienced laparoscopy in Institute of Protection of Mother and Newborn during January 1996 to December 1999. The results: applications of laparoscopy are rapidly increasing, both numbers and types. The diseases applied laparoscopy mainly were infertility (44.5%), ovarian cysts (24.0%), and ectopic pregnancy (22.8%). The complications of laparoscopy were very low

Remarks about laparoscopy during 1996-1999 period in Institute of Protection of Mother and Newborn (IPMN)

Remarks about laparoscopy during 1996-1999 periods in Institute of Protection of Mother and Newborn (IPMN). Laparoscopy for infertility had the highest rate (44.5%) with some technique: ovariotubal plastic surgery, polycystic ovary surgery…. Laparoscopy for ovarian cysts accounted for 24% with removed follicular cyst or ovariotomy procedure. The rate of laparoscopy for ectopic pregnancy was 22.8% mainly by gestational ovariotubal procedure. The rate of preserving ovary in ectopic pregnancy was very low, only 20% of total cases. The complications of laparoscopy were very low