ABSTRACT
Background: Early pregnancy loss is defined as the termination of pregnancy before 20 weeks' gestation or with a fetal weight of <500 g. Of those that are recognized, 15-20% result in spontaneous abortions [SABs] or ectopic pregnancies. Approximately 5% of couples trying to conceive have two consecutive miscarriages, and approximately 1% of couples have three or more consecutive early pregnancy loss. Increased levels of homocysteine may be due to inadequate dietary intake of folate and vitamin B12 and inherited defects within the methionine-hmocysteine pathway such as MTHFR c677T gene polymorphism. Hyperhomocysteinemia is associated with the syndromes of repeated miscarriage
Objective: The aim of the current study is to estimate the serum levels of Homocystine and vitamin B12 and their relation to prevalence of recurrent spontaneous abortion in pregnancies with history of recurrent miscarriage
Subjects and Methods: In this study 80 pregnant women classified into two groups: GroupI:60 consecutive pregnant women who had suffered from two or more miscarriages, and Group II: 20 pregnant women with no history of abortion .The following estimations were done [for each woman in the study]: serum homocysteine and vitaminB12 were estimated using ELISA technique
Results: Results showed a significant increase in serum level of homocysteine in the first group compared to control group[p<0.0001] and a low serum level of vitamin B12 which was significantly decreased in the study group [group 1] compared to control group[p<0.0001]. Moreover, Our results showed a negative correlation between homocysteine and vitamin B12
Conclusion: The elevated serum homocysteine levels during pregnancy were significantly associated with recurrent pregnancy losses. Moreover, reduced serum vitamin B12 level was considered a risk for recurrent miscarriage. Homocysteine is most sensitive and specific parameters in the recurrent pregnancy losses
ABSTRACT
Background: Chemerin is a newly discovered adipokine that regulates adipocyte development, metabolic function as well as immune function. Anti-Mullerian hormone [AMH] is a dimeric glycoprotein that belongs to the transforming growth factor-beta family
Aim of the study: was to evaluate the serum levels of Chemerin and AMH in one trial to show their possible involvement in the pathogenesis of PCOS and their association with obesity
Subjects and methods: This study was performed on eighty subjects divided into 4 groups: Group I: 20 non-obese control [BMI< 30], Group II: 20 non-obese patient with polycystic ovarian syndrome [BMI< 30], Group III: 20 obese Control [BMI >/= 30] and Group IV: 20 obese patient with PCOS [BMI >/= 30]. Serum levels of Chemerin and AMH were estimated by ELISA
Results: Results showed a significant increase in serum levels of Chemerin and AMH in [non-obese and obese] PCOS groups when compared to their controls. Results showed also a significant increase in the serum level of Chemerin in obese PCOS group when compared to non-obese PCOS group. While a significant decrease in serum level of AMH in obese PCOS group when compared to non-obese PCOS group. There were a significant positive correlation between Chemerin and AMH in PCOS groups
Conclusion: Chemerin together with AMH may be used as a biomarker for early detection of PCO and Clinical parameters as Infertility and Hyper and rogenism in women with PCOS