ABSTRACT
Objective: To explore the intervention effect of Yishenbaozhen Recipe (YSBZR) on the renal anemia in rats with chronic renal failure and the related mechanism. Methods: Totally 43 rat models of chronic renal failure were produced by two-step 5/6 nephrectomy. The study was randomly divided into 5 groups: normal control group (n=9), model group (n=8), YSBZR high-dose group (n=9, 46. 5 g/[kg • d]), YSBZR low-dose group (n=9, 15. 6 g/[kg • d]) and Niaoduqing group (n=8, 2.5 g/[kg • d]). YSBZR was given intragastrically on a daily bases for 6 weeks, and then the serum erythropoietin (EPO), serum creatinine (SCr), blood urea nitrogen (BUN), hemoglobin (Hb), red blood cell count (RBC), and mean corpuscular volume (MCV) were examined. Results: Compared with the model group and Niaoduqing group, YSBZR high-dose group and low-dose group had significantly higher Hb, RBC and EPO levels (P<0.05), and had similar MCV levels. YSBZR high-dose and low-dose groups had significantly decreased SCr and BUN levels compared with the modll group (P<0.01), and had similar levels when compared with Niaoduqing group. Conclusion: YSBZR has interventional effect on renal anemia, which might be related to decreasing nitrogen retention, reducing the toxin-induced inhibition of hematopoietic system, and increasing EPO level.
ABSTRACT
Objective To investigate the therapeutic effects of Erqi decoction (EQD) for treatment of acute radiation enteritis in SD rats and the related mechanism. Methods A total of 53 SD rats were randomly divided into normal group model group, EQD group and Baitouwengtang (BTWT) group, with 8 rats in the normal control group and 15 in each of the rest groups. Rats in the latter three groups were exposed to a single dose of 10 Gy 6 MV higher-energy X-rays on the abdominal region to establish the acute radiation enteritis models. Then all the rats were intragastrically administered with corresponding agents for a consecutive of 7 days. Then 10 ml ileum samples in ileocecal junction were obtained from each animal under general anesthesia. The morphologic indiceswere examined by light microscopy and the image analysis system. The nitric oxide (NO) levels, superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in the small intestinal homogenates were measured by spectrophotometer. Results The EQD group had significantly higher villus height ([243±9] vs [211 ±21] μm), deeper crypt ([171 ± 12] vs [142±10] μm), thicker mucosa ([460±21] vs [420±37] μm) and entirewall thickness ([609±19] vs [569 ±21] (μm) than the model group (P<0. 05), and the above parameters were not significantly different between the EQD group and the BTWT group. EQ group had significantly lower NO level ([0. 88 ± 0. 11] vs [1. 65 ±0. 12] μmol/g), higher MDA level ([3. 20±0. 12] vs [4. 75±0. 24] nmol/mg) and lower SOD activity ([212±13] vs [150± 11] U/mg, P< 0. 05) in the intestine compared with the model group (P<0. 05). EQD group had significantly lower NO and MDA levels and higher SOD activity compared with the BTWT group (P<0. 05). Conclusion It is indicated that EQD has protective effect against intestine mucosa damage in acute radiation enteritis rat model by reducing the intestinal NO generation, improving intestinal SOD activity, reducing MDA generation. Anti-free radical injury may be one of the mechanisms for treatment of acute radiation enteritis.