Multivariate Logistic analysis of ICU sydrome and establishment of risk model / 中国实用护理杂志

Object To investigate the main risk factors of ICU sydrome,and establish Logistic regression model to evaluate the accuracy rating、sensitivity and specificity for predicting ICU sydrome.Methods Investigated all patients hospitalized in ICU from June to July of 2007.Data collected include age,gender,religion,family income,education degree,primary disease,character type,APACHE II score and et al.Case-control study Was used to retrospectively investigate the clinical data of ICU sydrome.Firstly,sillgle factor analysis was used in 21 clinical variables.Then,Multivariate Logestic regression analysis was used in selected variables.Results Twelve clinical variables,including age,gender,family income,health care cost wiping out degree,whether or not having family member'death,whether or not encountering other patients'death,length of stay in ICU,whether or not receiving ventilator theraphy,sleep quality,score of APACHE II,time with disease,character type.By Logestic regression analysis,it WSS revealed that there were 6 significant factors for ICU sydrome in diminishing sequence as whether or not receiving ventilator theraphy,length of stay in ICU,sleep quality,gender,time with disease and whether or not encountering other patientsMeath.Logestic regression equation was as following:P=1/[1+Exp∑(31.425+2.611X1+8.188X2-2.677X3+7.474X4+4.312X5+6.153X6)].The accuracy rating、sensitivity and specificity of the Logestic model was 82.37%.79.42% and 85.13% respectively.Conclusions Whether or not receiving ventilator theraphy,length of stay in ICU,sleep quality,gender,time with disease and whether or not encountering other patients'death were the main risk factors of ICU sydrome.The Logestic regression model based on these factors is reliable in predicting ICU sydrome.

Comparison of human cell IFN-β production induced by bluetongue virus dsRNA and polyinosinic polycytidylic acid / 中华微生物学和免疫学杂志

Objective To investigate the capability of bluetongue virus(BTV)dsRNA inducing IFN-β from human cells.Methods Artificial complex interfemn inducer polyinosinic-polycytidylic acid (PolyI:C).BTV and BTV dsRNA were added to A549(human lung cancer cell)and HEL(human lung normal cells)culture system in difierent concentrations.IFN-β in culture median was detected by ELISA.Results Though all of the 3 reagents could induce IFN-β,BTV dsRNA significanay induced the highest level of IFN-β.The production of IFN-β was induced by BTV dsRNA in dose dependence.BTV dsRNA induced IFN-β level from HEL Was higher than that from A549(P<0.05).Conclusion BTV dsRNA Can induce IFN-β from human cells effectively,which shows its potential of an endogenous IFN-β inducer.