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The incidence of nonalcoholic fatty liver disease (NAFLD) has been increasing in China year by year, and NAFLD has become the second most common liver disease after viral hepatitis. Its development is closely associated with insulin resistance and oxidative stress and NAFLD is one of the important components of metabolic syndrome. Ghrelin is a newly discovered endogenous ligand for growth hormone secretagogue and has broad biological functions. Ghrelin not only affects the metabolic process of glucose and lipids, but also plays an important role in the development and progression of NAFLD. In this review, the role of endogenous Ghrelin expression in the development and progression of NAFLD is briefly summarized and the Ghrelin regulation in the development and progression of NAFLD is pointed out, which will provide new approaches to the clinical treatment of NAFLD.
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Objective To explore the association of expression of transporter associated with antigen processing(TAP1),tumor necrosis factor-alpha(TNF-α)with occurrence and development of HBV-related hepatocellular carcinoma(HCC).Methods The expression of TAP1 and TNF-α in 35 liver carcinoma tissues,and 10 normal liver tissues were detected with immunohistochemical EnVision method,The expression and clinical significance of TAP1,TNF-α in HBV-related HCC were analyzed.Furthermore,the association of respective expression quantity with pathological typing of HCC was analyzed.Results The expression of TAP1,TNF-α was negative in hepatocytes of normal liver tissues.91.4％ of HCC tissues expressed TAP1.There was significantly statistical difference in expression of TAP1 between HCC tissues and the normal hepatic tissues(P ＜ 0.05);60％ of HCC tissues expressed TNF-α.There was statistical difference in expression of TNF-α between HCC and the normal hepatic tissues(P ＜ 0.05),and the positive expression rate of TNF-α in well-differenciated HCC tissues was significantly higher than that in poorly-differenciated ones(P ＜ 0.05).Conclusion TAP1-related MHC-Ⅰ restricted antigen processing pathway was possibly normal in HBV-related HCC.The expression quantity of TNF-α was associated with the occurrence and degree of pathological differentiation of HBV-related HCC.
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Objective To evaluate the therapeutic effect of sho-saiko-to silymarin on liver fibrosis due to chronic hepatitis B.Methods 90 patients with liver fibrosis due to chronic hepatitis B virus(HBV) were randomly divided into therapy group and control group.50 patients in therapy group were given sho-saoko-to and silymarin;While 40 patients in control group were given silymarin only,they were all treated for 6 months.The changes were observed including liver function,serum liver fibrosis and virology indexes before and after treatment in two groups.Resuits Indexes of liver function and serum liver fibrosis in therapy group were improved obviously(P<0.05 or P<0.01),while better clearance to HBV is comparable to control group(P<0.05).Conclusion The treatment with sho-saiko-to combined silymarin on liver fibrosis due to chronic hepatitis B had explicit clinical effect.
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Objective:The purpose of this study is to determine levels of Ghrelin,TNF-α,IL-1 and IL-6 in peripheral blood of patients with chronic hepatitis B,liver cirrhosis,and non-alcoholic fatty liver disease which diagnosed at different extent of damage,and based on it to have a further study on the relevance between Ghrelin and inflammatory cytokines in peripheral blood in above diseases.Methods:Ghrelin,TNF-α,IL-1 and IL-6 in peripheral blood were determined by ELISA method.Results:In patients with chronic hepatitis B andhepatic cirrhosis,Ghrelin level increased significantly as compared with that in normal control group (P＜0.05,P＜0.01).Ghrelin levels in decompensated liver cirrhosis (degree B and C)groups were much higher than in chronic hepatitis B group (P＜0.05). Ghrelin level in group of non-alcoholic fatty liver disease decreased significantly as compared with that in normal control group (P<0.01). Ghrelin and inflammatory cytokines,including TNF-α,IL-1 and IL-6 showed a positive relevances in groups of chronic hepatitis B and liver cirrhosis,but not in NAFLD group which showed a negative correlations.Conclusion:Ghrelin has a higher level in peripheral blood of patients with chronic hepatitis B and liver cirrhosis,but it has a lower level in patients with non-alcoholic fatty liver disease,suggesting correlation of Ghrelin level with the occurrence and the procession of chronic liver diseass.
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Objective To investigate the effect and clinical significance of HBV replication on the serum levels of IL-12 and IL-18 in patients with hepatitis B-related cirrhosis.Methods The levels of HBV-DNA and IL-12 and IL-18 in peripheral blood from 136 patients with different clinic types chronic hepatic diseases(102 patients with chronic hepatitis B,34 patients with hepatitis B-related cirrhosis ),95 HBV carriers and 20 healthy people (as control group) were respectively detected with ELISA and real time PCR.Results The levels of IL-12 and IL-18 in chronic hepatitis B and hepatitis B-related cirrhosis groups were significantly increased compared with control group(P0.05); and there was positive relationship between prevalence of HBV replication and IL-12,IL-18 levels in different chronic hepatic disease groups(r=0.74,P
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Objective:To explore antitumor effects of combined interleukin 2(IL 2)gene and tumor necrosis factor(TNF ?)gene therapy in nude mice model with hepatocellular carcinoma.Methods:Nude mice model with hepatocellualr carcinoma was established with BelH7402 cell line.Plasmid vector was used to transfect BelH7402 and nude mice model with the gene for human IL 2 or TNF ? alone or in combination by lipofectamine reagent and tumor size in nude mice model with BelH7402 carcinoma was measured before and after treatment to evaluate the response of the different treatment and control groups.Results:Tumor and BelH7402 transfected with IL 2 or TNF ? gene by liposome mediated were successful.Growth rates of BelH7402 transfected with IL 2 or TNF ? gene were not significantly changed,growth rate of tumor in nude mice model was significantly inhibited in combined IL 2 and TNF ? genetherapy group as compared with the other groups(P
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Objective:To investigate the changes of Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues of patients with heptocellular carcinoma and its clinical significance.Methods:Th1/Th2 subsets and cellular factor were detected by ELISPOT and double-sandwich ELISA.Results:Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues of patients with heptocellular carcinoma,Th1 sebset expressing cellular factor IFN-?,IL-2,IL-12 were significantly lower,and Th2 sebset expressing cellular factor IL-4,IL-10 were significantly higher than those of the normal control group.PMBC in patients with heptocellular carcinoma producing Th1 sebset cytokins deduced by PHA and IL-12 were significantly higher and producing Th2 sebset cytokins were significantly lower than those in the three month after operation.Conclusion:Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues were of imbalance and immune functional status of patients with hepatocellular carcinoma was inhibitory.Therefore,it was nessassory to enhance immune functional status of patients during treatment.
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Objective: To observe relation among sICAM-1 level in the sera of patients with chronic viral hepatic disease, viral replication and the clinical significance. Methods: The level changes of sICAM-1, IL-1,IL-8 and TNFa in the sera of patients with chronic hepatitis B(CH),chronic severe hepatitis B(CSH) and hepatocirrhosis (HC) were detected by ELISA. Results: The levels of sICAM-1,IL-1,IL-8 and TNFa in sera of the patients with chronic viral hepatic disease were significantly higher than those of healthy control group(P<0.01). The bilirubin level in the sera of patients with chronic viral hepatic disease of different clinical types positively correlated with sICAM-1 and cytokine activities. The sICAM-1 level in the sera of HBV-DNA or HBeAg positive patients was significantly higher than those of negative patients(P<0.05,P<0.01). Conclusion: The sICAM-1 level and activities of cytokine IL-1,IL-8 and TNFa as well as the bilirubin level in the sera of the patients can reflect the necrosis degree of hepatocytes, and the sICAM-1 level and activities of IL-1,IL-8 and TNFa were related to state of HBV carrier or the active degree of HBV in patients with hepatic disease.
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Objective:To evaluate the effect of Helicobacter pylori infection on hyperdynamic circulatory state and portal hypertensive gastrropathy in liver cirrhotic patients.Methods:Helicobacter pylori infection,IL-1,IL-8,TNF-?,Endotoxin,NO,ET,anti-VacA and anti-CagA levels were determined in cirrhotic patients by using rapid urease test,()~(14)C-urea expiratory test,immunoimpression test,nitrite colorimetric test and ELISA.The parameters of portal venous system flow were determined by doppler ultrasonography.Results:HP prevalence in patients with liver cirrhosis was 77.1%,anti-VacA and antiCagA positive rate was 60.4% in common.IL-1,IL-8,TNF-?,Endotoxin,NO and ET levels in anti-VacA and anti-CagA positive group were significantly higher than in anti-VacA and anti-CagA negtive group.The amount of splenic venous flow(SVF) and superior mesenteric venous flow(SMVF)in anti-VacA and anti-CagA positive group was greater and the amount of portal venous flow(PVF) was smaller than those in negtive group.Conclusion:There was the effect of Helicobacter pylori infection on hyperdynamic circulatory state and portal hypertensive gastrropathy in liver cirrhotic patients.
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Objective:To investigate the association of human leucocyte antigen-DRB1 alleles with chronic hepatitis B and hepatic cirrhosis in Han population of Jilin area.Methods:HLA-DRB1 alleles in 61 patients with chronic hepatitis B,44 patients with hepatic cirrhosis,32 spontaneous rehabilitees after HBV infectin,and 50 healthy controls were analyzed by using polymerase chain reaction/sequence specific primer technique.Results:The frequency of HLA-DRB1*1201-3 allele in chronic hepatitis B group was higher than that in healthy control group(17.21% vs.8.00%,P=0.0427,RR=2.391).The frequency of HLA-DRB1*0701 allele in chronic hepatitis B group and hepatic cirrhosis group was markedly higher than that in healthy controls group(11.48 % vs.2.00%,P=0.006 6,RR=6.35;12.50% vs.2.00%,P=0.004 6,RR=7.00)and in spontaneous rehabilitee group (11.48% vs.1.56%,P=0.0183,RR=8.17;12.50% vs.1.56%,P=0.0136,RR=9.00).No significant differences frequencies of the other HLA-DRB1 alleles were observed among the four groups.Conclusion:Alleles of HLA-DRB1*1201-3 and HLA-DRB1*0701 are closely associated with chronic hepatitis B and maybe susceptible genes or linkage genes of chronic hepatitis B.HLA-DRB1*0701 is also closely associated with hepatic cirrhosis and maybe a susceptible gene or linkage gene-posthepatitis B-cirrhosis in Han population of Jilin area.
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Objective:To analyse clinical signification of four kinds of autoimmune antibodies in the patients with viral hepatitis.Methods:ANA RF,anti TM and dsDNA Ab in sera of patients with viral hepatitic disease were detected by means of indirect immune fluerscence(IIF) and ELISA.Results:12 of 63 (19.1%) were only one kind of autoimmune antibody in sera of patients with hepatitis B and 12 of 40(30%) of patients with hepatitis C.There were significantly higher than those of alcoholic and drug hepatitis group(P
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Objective:To evaluate p53,c erbB 2,p21 and nm23 oncoprotein expression in diagnosis and treatment of gastric cancer.Methods:The expression of p53,c erbB 2,p21 and nm23 oncoproteins was detected with immunohistochemical method in 63 surgically resected specimens and its endoscopic biopsy specimens of gastric cancer.Results:The positive rates of p53,c erbB 2,p21 and nm23 oncoprotein expression were 37 6%～46 2%,34 6%～56 8%,37 8%～61 5%,70 3%～30 8% respectively.Oncoprotein expression was not observed in non tumor endoscopic biopsy specimens.The expression of c erbB 2,p21 was correlated with grade of tumor differentiation and the expression of p21,nm23 oncoproteins was correlated with the depth of tumor invasion and clinical different stage,namely tumor metastasis.Positive rates of p53,c erbB 2,p21 and nm23 oncoproteins between biopsy and resected specimens was of coincidence.Conclusion:Determination of p53,c erbB 2,p21 and nm23 oncoprotein expression was might be useful in diagnosis and treatment of gastric cancer,differentiating benign and malignant tumor and clinical stages,of gastric cancer. [
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Objective:To observe clinical significance between changes of OPN,IL-18 levels and relation ship with hepatic function in patients with chronic hepatic disease infected by HBV.Methods:The levels of OPN,IL-18 in peripheral blood of 102 patients with chronic hepatitis B,34 patients with hepatitis B-related cirrhosis,95 HBV carriers and 20 healthy people as the control group were respectively detected with ELISA.Results:The levels of OPN and IL-18 in chronic hepatitis B and hepatitis B-related cirrhosis groups were significantly increased compared with that of health control group(P
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Abstract Objective: To observe relationship among sICAM-1、TGF-?1, and cellular factor level changes in the sera of patients with chronic viral hepatic disease, viral replication and clinical significance.Methods;The sICAM-1 .TGF-?1, ,IL-1,IL-8 and TNFa level changes in the sera of patients with chronic hepatitis B(CH), chronic severe hepatitis B(CSH) and hepato-cirrhosis(HC) were detected by FJ-ISA. Re-sults: The sICAM-1, IL-1, IL-8 and TNFa levels in sera of patients with chronic viral hepatic disease were significantly higher than those of healthy group( P
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T lymphocyte subpopulation and level of soluble interleuckin 2 receptor in peripheral bloodand serum of primary hepatocellular carcinoma and donor were tested.The results show that de-crease of T lymphocyte subpopulations is related with increase of level of soluble interleuckin2 re-ceptor in patients with primary hepatocellular carcinoma.