ABSTRACT
OBJECTIVE:To investigate the effects of total paeony glycoside (TPG) on the expression of tumor suppressor gene in lung cancer model rats. METHODS:90 rats were randomly divided into normal group,model group,positive control group [cyclophosphamide,50 mg/(kg·d)] and TPG low-dose,medium-dose and high-dose groups [50,100,200 mg/(kg·d)] with 15 rates in each group. Except for normal group,other groups were given disposable infusion of carcinogenic iodized oil via left lobe bronchus to induce lung cancer model. After modeling,those groups were given relevant medicine intravenously from Monday to Friday,while normal group and model group were given constant volume of normal saline intravenously for consecutive 16 weeks. The expression of multidrug resistance associated protein(MRP),human multidrug resistance gene(MDR1),P21 and P16 mRNA in lung tissue of rats were determined by RT-PCR;the expression of P53 protein in lung cancer tissue was determined by IHC method.The rate of positive expression was calculated,and pathological change of lung tissue was observed. RESULTS:Com-pared with normal group,the expression of MRP,MDR1,P21,P16 mRNA and P53 protein(positive rate of 66.67%)in lung tis-sue was increased significantly in model group (P<0.05);compared with model group,the expression of MRP,MDR1,P21, P16 mRNA and P53 protein (positive rate of 46.67%,46.67%,40.00%,13.33%) decreased in positive control group,TPG low-dose,medium-dose and high-dose groups in dose-dependent manner,and the decrease of TPG medium-dose and high-dose groups were more significant than that of positive control group (P<0.05);there was statistical significance in above indexes among TPG groups(P<0.05). CONCLUSIONS:TPG could inhibit the expression of MRP,MDR1,P21,P16 gene and P53 pro-tein in lung cancer model rats significantly.
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OBJECTIVE To screen for a simple,accurate and no-traumatic detecting method of Helicobacter pylori(Hp). METHODS Hp in gastric fluid and dental plaque was detected with fluorescent antibody method,bacterial culture,urease test and Hp diagnosis card at the same time in 62 cases with gastric and duodenal disease.The gather of gastric fluid applied the capsule method. RESULTS The detective rates of Hp in gastric fluid by four methods were 85.5%,9.7%,61.3%,and 56.5%;the detective rates of Hp in dental plaque by four methods were 88.7%,25.8%,69.4%,and 90.3%,respectively. CONCLUSIONS Fluorescent antibody method combined with capsule method detecting Hp in gastric fluid is specific,sensitive and without trauma.Thus,it is suggested to be used clinically.
ABSTRACT
<p><b>OBJECTIVE</b>To provide further pathogenic evidence of Granulocytic ehrlichia infection in China.</p><p><b>METHODS</b>Specific primers derived from 444-Epank gene were used to amplify Granulocytic ehrlichia DNA from specimens of ticks, animals and human blood. PCR products of ticks were cloned and sequenced.</p><p><b>RESULTS</b>444 bp specific DNA fragments were amplified from 2 of 62 pools of Ixodes persulcatus collected from Heilongjiang province and 1 of 129 blood specimens from forest workers in Inner Mongolia. Eight animal specimens were negative. PCR products from ticks were then cloned and sequenced. It differed at 23 positions in comparison to American strain (AF047897) with 94.9% homology. The homology of deduced ammonia was 88.44%.</p><p><b>CONCLUSION</b>Our findings further confirmed that Granulocytic ehrlichia infection did exist in China.</p>