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1.
EMJ-Egyptian Medical Journal [The]. 1990; 7 (9): 555-9
in English | IMEMR | ID: emr-16256

ABSTRACT

The objective of this study was to evaluate two new serologic assays for detecting HIV-form 1 and HIV-2 positive and negative sera. A total of 154 human sera from Egypt and African countries was tested using a synthetic peptide-based passive hemagglutination assay [PHA] and a rapid dot-blot recombinant test [Test-Pack], both of which are marketed as HIV-1/HIV-2 combination assays. The results indicated that the PHA identified all HIV-1 and HIV-2 positive sera, although some reacted only weakly. It produced one false positive reaction. The Test-Pack correctly detected all positive sera and three false negatives. All tests ran concurrently. Both identified HIV-2 positive antibodies and HIV-1 positive antibodies sera


Subject(s)
HIV-1 , HIV-2
2.
Journal of the Egyptian Public Health Association [The]. 1990; 65 (1-2): 1-10
in English | IMEMR | ID: emr-16685

ABSTRACT

Our objective was to evaluate two new serologic assays for detecting HIV-1 and HIV-2 positive and negative sera. A total of 154 human sera from African countries were tested using a synthetic peptide-based passive hemagglutination assay [PHA] and a rapid dot-blot recombinant test [Test-Pack], both of which are marketed as HIV-l/HIV-2 combination assays. Results indicated that the PHA identified all HIV-1 and HIV-2 positive sera, although some were only weakly reactive. It produced one false positive reaction. The Test-Pack correctly detected all positive and negative sera, although several negative sera produced extremely weak reactions. Both tests detected two HIV-2 positive sera which were non-reactive by HIV-1 screening assays


Subject(s)
Biological Assay
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