ABSTRACT
The objective of this study was to evaluate two new serologic assays for detecting HIV-form 1 and HIV-2 positive and negative sera. A total of 154 human sera from Egypt and African countries was tested using a synthetic peptide-based passive hemagglutination assay [PHA] and a rapid dot-blot recombinant test [Test-Pack], both of which are marketed as HIV-1/HIV-2 combination assays. The results indicated that the PHA identified all HIV-1 and HIV-2 positive sera, although some reacted only weakly. It produced one false positive reaction. The Test-Pack correctly detected all positive sera and three false negatives. All tests ran concurrently. Both identified HIV-2 positive antibodies and HIV-1 positive antibodies sera
Subject(s)
HIV-1 , HIV-2ABSTRACT
Our objective was to evaluate two new serologic assays for detecting HIV-1 and HIV-2 positive and negative sera. A total of 154 human sera from African countries were tested using a synthetic peptide-based passive hemagglutination assay [PHA] and a rapid dot-blot recombinant test [Test-Pack], both of which are marketed as HIV-l/HIV-2 combination assays. Results indicated that the PHA identified all HIV-1 and HIV-2 positive sera, although some were only weakly reactive. It produced one false positive reaction. The Test-Pack correctly detected all positive and negative sera, although several negative sera produced extremely weak reactions. Both tests detected two HIV-2 positive sera which were non-reactive by HIV-1 screening assays