[In vitro evaluation of the antimicrobial effectiveness of zataria multiflora as an irrigant in infected root canals with enterococcus faecalis]

Given the importance of bacteria in the development of periradicular lesions, the eradication of the root canal infection is paramount in endodontic treatment. The use of chemical irrigants during chemomechanical canal preparation is important for disinfection and cleaning of the canal system. The aim of this in vitro study was the evaluation of effectiveness of 1% and 2% essential oil of Zataria multiflora as an irrigant in the elimination of Enterococcus faecalis from the infected root canals. The antibacterial effects of the irrigants in vitro were examined after 15 min, in freshly extracted human teeth with single roots, the canals of which were infected by E. faecalis. 1.2ml of 2.5%NaOCl. 2 2ml of 1% Zataria multiflora. 3.2ml of 2% Zataria multiflora. 4.2ml of sterile normal saline [as control] Paper points used to sample bacteria from the root canals were transferred to tubes containing 5ml of brain heart infusion [BHI] broth. The tubes were incubated and the appearance of broth turbidity was indicative of bacteria remaining in the root canal. The data obtained were analyzed statistically for differences using the chi-squared test, comparing pairs of groups [p < 0.05]. There was no statistically significant differences between the experimental groups [p >0.05]. However, the experimental irrigants were significantly more effective than the saline solution [control group] in disinfesting the root canal. It seems that essential oil of Zataria multiflora was effective at killing E. faecalis. Application of this plant essential oil as root canal irrigants may be recommended following extensive ex vivo and in vivo experiments. The findings recommend further studies of the properties of Zataria multiflora such as toxicity, tissue solving ability, and biocompatibility to be applied for clinical use

[Antimicrobial activity of different concentrations of essential oil of Zataria multiflora on Enterococcus faecalis]

The main goal of endodontic treatment is eradication of microorganisms and their byproducts from the root canal system. The use of chemical irrigants during chemo-mechanical canal preparation is important for disinfection and cleaning of the canal system. The aim of this in vitro study was the assessment of bacterial growth after contact with several concentrations of essential oil of Zataria multiflora in varying time intervals. The essential oil of Zataria multiflora were serially diluted 8 folds and a fixed volume of the stationary phase culture of E.faecalis was added to each diluted solution, for varying intervals of 1, 5 and 15 minutes. 100 micro l of the contents of each tube were transferred to SF broth and incubated for 48h at 370c. Results of bacterial growth were recorded at the end of the incubation period. 2% solution of essential oil of Zataria multiflora at all intervals inhibits growth of E.feacalis. 1% solution of it showed antibacterial effect in 5, and 15 minutes. The concentrations under 0.5% dilution of Zataria multiflora did not eliminate E.faecalis at any time intervals. It seems that 1% and 2% solutions of essential oil of Zataria multiflora were effective at destroying E.faecalis. Application of this plant essential oil may be recommended for root canal irrigant following extensive ex-vivo and in-vivo experiments