[ prevalence of thyroid pathological lesions of in 202 autopsic samples obtained at Isfahan forensic medicine center]

Considering the influence of geographical, genetic and ethnic factors on the prevalence of thyroid pathological lesions, this study was performed to assess the prevalence of pathological lesions in thyroid autopsic samples obtained from adult cadavers of Isfahan Forensic Medical Center. In this study, 202 thyroid glands from cadavers with no evidence of thyroid diseases, removed during autopsies at Isfahan Forensic Medicine Center, were analyzed. After gross studies, thyroid glands were fixed in 5% formalin for 72 hrs, and then 3-6 sections were obtained from each gland. Tissue sections were microscopically studied by two pathologists for histopathological lesions. Among 202 thyroid glands [removed from 159 males and 43 females], 131[64.85%] were histopathologically normal, and goiter [nodular, and diffuse], Hashimoto's thyroiditis, lymphocytic thyroiditis, follicular adenoma and papillary carcinoma were observed in 41 [20.3%], 10 [4.95%], 12 [5.94%], 2 [1.49%] and 12 [5.94%] of the thyroid glands, respectively. Twenty-six glands [12.23%] had nodules sized

Effect of human sperm MTT viability assay on outcome of intraycytoplasmic sperm injection

Sperm MTT Viability Assay has been shown to be a suitable test for differentiation of viable from non-viable sperms. In this procedure MTT is converted to observable purple MTT Formazan by mitochondrial dehydrogenase in the midpiece region and therefore viable sperms can be distinguished which makes this test suitable for ICSI. Therefore, in order to study the effect of MTT positive sperms on fertilization, cleavage and blastocyst formation, 109 fresh human oocytes [metaphase II] were divided in to two groups; one group was injected with MTT positive sperms and the other one was taken as control. The results of study showed that there is not significant difference with respect to fertilization, cleavege and blastocyst formation between these two groups. Therefore, if MTT proves to be nether mitogenic nor teratogenic, sperm MTT viability assay might be useful for ICSI in patients with absolute or severe asthenospermia, especially in cases with tail abnormality