[Appointment of correlation between stroke and serum level of homocysteine, folic acid and vitamin B[12]]

Background: a stroke, or cerebrovascular accident [CVA], occurs when blood supply to part of the brain is disrupted, causing brain cells to die. Often by a blood clot or a fatty deposit due to atherosclerosis. CVA is third cause of death in the world and the most common causes of neurologic dysfunctions. There are different risk factors associated with CVA including systolic or diastolic hypertension, hyperlipidemia, smoking, alcohol abuse. Moderately increased concentration of homocysteine without homocystuinoria associated with increase of atherosclerosis, CVA and over ally mortality. The most common form of hyperhomocysteinemia is genetic. Increased serum level of homocysteine may reflect deficiency of folate, vitB6 and vitB[12]

Materials and Methods: to determine the relationship between serum level of homocysteine, folic acid and vitB[12] and CVA this study was done at a case control survey in 2008 in 40 known case of CVA and 40 control cases are selected among the out patient of neurology clinics of neurologist in kashan city. Demographic data and other inhumation [age, sex, ...] match at case and control groupe. 2cc venous blood of each patient tested for serum level of homocysteine folic acid and vitB[12] [with HPLC] and of all cases and control classified and analyzed

Results: among 40 CVA cases 52/5 female [21cses] 47/5 male [19 cases] and in control groupe 50 female [20 cases] and 50 male [20 cases]. The mean of the ages at cases attached was 67/15 and in control groups was 63/5 and all of the cases and controls was 65/32 and there was a not meaningful difference between two groups. In this study the mean of serum level of homocysteine at case attached was 15/56 +/- 4/69 µmol/l and in control group was 10/15 +/- 4/7 µmol/l And there was meaning full difference between two groups [P<0/05]. The mean of serum level of folic acid was 8/405 +/- 5/33 ng/ml in case groups was and in control groups 10/87 +/- 8/02ng/ml there was meaning full difference between two groupes [P<0.005]. The mean of serum level of vitB[12] in cases attached was 389.02 +/- 208.02 pg/ml and in control groups was 522.82 +/- 218.3 pg/ml and there was meaning full difference between two groups [P<0.05]

Conclusions: this study showed correlation between CVA and serum level of homocysteine, folic acid and vitamin B[12]. It is recommended to conduct prospective studies to evaluate the cause and effect between these supplements and stroke and practical ways of prevention of CVA

[Appointment of correlation between stroke and serum level of homocysteine, folic acid and vitamin B[12]]

A stroke, or cerebrovascular accident [CVA], occurs when blood supply to part of the brain is disrupted, causing brain cells to die. Often by a blood clot or a fatty deposit due to atherosclerosis. CVA is third cause of death in the world and the most common causes of neurologic dysfunctions. There are different risk factors associated with CVA including systolic or diastolic hypertension, hyperlipidemia, smoking, alcohol abuse. Moderately increased concentration of homocysteine without homocystuinoria associated with increase of atherosclerosis, CVA and over ally mortality. The most common form of hyperhomocysteinemia is genetic. Increased serum level of homocysteine may reflect deficiency of folate, vitB[6] and vitB[12]. To determine the relationship between serum level of homocysteine, folic acid and vitB[12] and CVA this study was done at a case control survey in 2008 in 40 known case of CVA and 40 control cases are selected among the out patient of neurology clinics of neurologist in Kashan city. Demographic data and other inhumation [age, sex,.] match at case and control group. 2cc venous blood of each patient tested for serum level of homocysteine folic acid and vitB[12] [with HPLC] and of all cases and control classified and analyzed. Among 40 CVA cases 52/5 female [21 cases] 47/5 male [19 cases] and in control group 50 female [20 cases] and 50 male [20 cases]. The mean of the ages at cases attached was 67/15 and in control group was 63/5 and all of the cases and controls was 65/32 and there was not meaningful difference between two groups. In this study the mean of serum level of homocysteine at case attached was 15/56 +/- 4/69 micro mol/l and in control group was 10/15 +/- 4/7 micro mol/l And there was meaningful difference between two groups [P<0/05]. The mean of serum level of folic acid was 8/405 +/- 5/33 ng/ml in case groups was and in control groups 10/87 +/- 8/02ng/ml there was meaning full difference between two groups [P<0.005]. The mean of serum level of vitB[12] in cases attached was 389.02 +/- 208.02 pg/ml and in control groups was 522.82 +/- 218.3 pg/ml and there was meaning full difference between two groups [P<0.05]. This study showed correlation between CVA and serum level of homocysteine, folic acid and vitamin B[12]. It is recommended to conduct prospective studies to evaluate the cause and effect between these supplements and stroke and practical ways of prevention of CVA

[Determination of P53 gene mutations in gastric cancer by PCR-SSCP]

Background and Objective: gastric cancer is the 2nd cause of cancer mortality after lung cancer. Approximately 12% of all cancer death are due to gastric cancer. Tumorgenesis is thought to be a multistep process involving a series of genetic changes in oncogenes and suppressor genes. The most common cancer-related genetic change known in human tumors is P53 mutation, particularly in gastric cancer. This study was done to determine P53 gene mutations in gastric cancer

Materials and Methods: this study was performed on 44 biopsy from patients with gastric cancer during 2002 in 3 hospitals in Tehran. For determination of P53 gene mutations was performed PCR-SSCP methods

Results: the patients group comprised 31 males and 13 females [Average age, 60.8 years Ranging from 34 to 84 years]. 36 cases [81.8%] intestinal type, 5 cases [11.4%] were diffuse type and 3 cases no defined. 44 gastric cancers of gastric tissues were screened for the mutations of P53 gene mutations in exons 5-8 using the PCR-SSCP analysis. After polyacrylamide gel electrophoresis, 9 patients [20.5%] showed an apparent electrophoretic mobility shift between the cancer and other normal samples. One mutation in exon 5 [11.1%], 2 were detected in exon 6 [22.2%], 3 were found in exon 7 [33.3%] and 3 were detected in exon 8 [33.3%]. The mutation rate was 7 of 36 [21.2%] in intestinal type and 2 of [40%] in diffuse type. No significant correlation between P53 gene mutations and age and genus was found

Conclusion: this investigation showed the rate P53 gene mutation [20.5%] in gastric cancer in our society