[Study of exon deletion in the dystrophin gene in individuals being diagnosed with duchenne muscular dystrophy in Ahvaz]

Duchenne muscular dystrophy is an X-linked genetic disorder resulting from mutation or deletion in the Dystrophin gene. The aim of this study was to evaluate the primary diagnosis of affected individuals that have been referred to the genetic lab of the Shafa hospital in Ahvaz. Progressive muscle weakness was present in all the patients. DNA from peripheral blood was extracted from affected patients and subsequent multiplex-PCR was performed to determine putative deletions in the Dystrophin gene. in 53% of cases were deletions identified in exons 44-51 in the Dystrophin gene and therefore the clinical diagnosis could be confirmed. On the other hand, we found no deletion in 47% of cases. it seems that the patients suffering Duchenne muscular Dystrophy in Ahvaz show, independent to their ethnicity, the gene inactivating deletion in the end part of the Dystrophin gene. These results would be used for the differential and for the prenatal diagnosis in the Khuzestan province

[Inhibition effect of antibiotics ciprofloxacin and ofloxacin and hormone beta-stradiol 17 valerat on the methanogenic activity of anaerobic biomass]

Antibiotics and hormones are excreted with other wastes following their influences on bodies. These substances can disturbed treatment process by their entery to the wastewater. In this study the inhibitory behavior of antibiotics Ofloxacin and Ciprofloxacin and hormone beta stradiol 17- valerat have been investigated on Specific Methanogenic Activity [SMA] of anaerobic biomass. Twenty one SMA tests were done using 120-mLvials in batch mode. In each vial, substrate, biomass and biogas were occupied 66, 17, and 17% [v/v], respectively. Each test longed in range of 15-30 days. Produced methane was measured by gas replacement with 2N KOH solution as CO2 absorbent. In this study, at the concentrations of 200, 500 and 1000 mg/L of antibiotic Ofloxacin, the methane production reduced to 45, 76 and 88 percent, respectively. Reduced methane production of 68, 81 and 88 percent was observed in Ciprofloxacin concentrations of 100, 200, and 500 mg/L, respectively. Cumulative methane at the concentrations of 0.1, 1, and 5 mg E2 /L was 66, 90, and 121 mL, respectively Antibiotic Ciprofloxacin at concentrations similar to the antibiotic Ofloxacin have a greater inhibitory effect on specific methanogenic activity of anaerobic biomass. Also, the hormone E2 at lower concentrations showed more inhibitory effect than other two antibiotics Ciprofloxacin and Ofloxacin