Modulation of human polymorphonuclear leukocytes oxidative metabolism by high temperature in vitro

In an attempt to evaluate the effect of the different temperatures on the oxidative metabolism of polymorphonuclear leukocytes [PMNs], the isolated or whole blood human PMNs were pre-incubated at different temperatures and incubation periods before estimating its oxidative metabolism using the luminol chemiluminescesne [CL] technique. At 38C, 39C or 40C preincubation temperatures, the phorbol myristate acetate [PMA] or opsonized zymosan stimulated PMNs showed stimulated CL responses in relation to control [37C]. On the other hand, preincubation of PMNs at temperatures higher than 40C caused inhibition to its respiratory burst in a temperature dependent manner. The viability of PMNs was reduced by incubating the cells at a temperature higher than 42C

Galactosamine [GaIN] inhibits luminol-dependent chemiluminescence of human polymorphonuclear leukocytes

The aim of the present study was to examine the effect of D- galactosamine HCl [GaIN] on the oxidative respiratory burst of isolated human polymorphonuclear leukocyte [PMNs]. GaIN added in vitro to PMNs caused a marked inhibitory effect on the luminol- dependent chemiluminescene [CL] induced by phorbol myristate acetate [PMA] on PMNs. The inhibitory effect produced by GaIN was both dose and time dependent when PMA was used to stimulate the oxidative burst of PMNs. The effect of GaIN on the isolated PMNs was partially irreversible, following washing of the GaIN-treated PMNs with phosphate buffered saline [PBS]. PMNs viability was not significantly altered by incubation with GaIN. Addition of lipopolysaccharide [endotoxin] to isolate PMNs in the presence or absence of GaIN did not alter the oxidative burst of PMNs. Addition of oxygen-free radical scavengers enhanced the inhibitory effect induced by GaIN on PMNs CL. In a cell free medium, GaIN has no inhibitory effect on CL induced by luminol, H2O2 and horse radish peroxidase. As a conclusion, results suggested that in vitro, GaIN has a remarkable inhibitory effect on the release of oxygen products from stimulated PMNs

Inhibition of human polymorphonuclear leukocyte function by lead

In an attempt to evaluate the direct effect of lead [Pb] on the physiological function of polymorphonuclear leukocytes [PMNL], isolated PMNLs were incubated with various concentrations of Pb [1-1000 micro M] for 0, 30, and 60min. The PMNL respiratory burst, phagocytosis and viability were examined in the presence and absence of Pb. The results show that Pb significantly inhibited the PMNL-chemiluminescence [CL] response stimulated with the soluble agent, phorbol myristate acetate [PMA] or a particulate agent, opsonized zymosan [OPZ]. The inhibitory effect of Pb on PMNL-CL responses is concentration, and time-dependent, and irreversible. Additionally, PMNL phagocytosis was significantly reduced when the cells were incubated with various concentrations of Pb. The PMNL viability was not altered when the cells were incubated with low concentrations of Pb [10-100 micro M]. It is concluded that Pb significantly depressed the physiological function of PMNL in vitro