Interaction of pesticide exposure level with some biochemical enzymes among fleld workers

Enzymatic studies were done on workers with different exposure levels to pesticides before and after pesticides application. The results showed a decrease in the activities of serdm acetyicholinesterase and monoamine oxidase. Acid phosphatase activity was increased accompanied by decreased ChE activity. Also, the liver function tests [SCOT, SGPT, AP] were affected. All these effects were more pronounced among the high exposure group after the pesticides application

Interaction of hepatic protein with anticholinesterase action of some aliphatic organophosphorus insecticides

Acute oral toxicity of Malathion, Dimethoate and Dichlorvos was determined 24 hours after treatment against male and female white rats. Malathion and Dimethoate were slightly more toxic against male rats than female rats. While, female rats were more susceptible than male rats for Dichlorvos. In addition, after chronic treatment, Dichlorvos was more pronounced in brain and liver ChE inhibition than Dimethoate and Malathion in male and female rats. Data demonstrated that, there was a marked inhibition of ChE after chronic feeding on each insecticide without any clinical signs of toxicity. On the other hand, results demonstrated that preincubation of Dichlorvos with rat hepatic protein reduced the inhibitory effect against male and female rat brain AChE at 1x10[-5] M either in the presence or the absence of NADPH. While the preincubation of Dimethoate and Malathion with hepatic protein enhanced their anti ChE effect at the same concentration in the absence of NADPH while in the presence of NADPH, their inhibitory power was reduced. On the other hand, it was quite clear that, there was a time-response relationship between piperonyl butoxide pretreatment on the brain anti-ChE action of the tested insecticides in rats