ABSTRACT
The protective effect of fresh aloe vera [AV] leaves extract on lindane [LD] ' induced hepatoxicity and genotoxicity was studied. Serum levels of hepatic enzyme markers: glutamate pyruvate transaminase [GPT], glutamate oxaloacetate transaminase [GOT], gamma glutamyl transferase [GGT] and alkaline phosphatase [ALP] were determined after oral administration of aloe vera leaves extract and lindane. The level of polychromatic erythrocytes was also observed. Pretreatment with aloe vera leaves extract at concentration of 1.0 ml/kg body weight significantly decreased [P<0.05] the serum levels of GPT [by 41.8%], GOT [by 36.5%], GGT [by 14.3%] and ALP [by 10.7%] induced by 100mg/kg body weight of lindane. The level of polychromatic erythrocytes observed was not statistically significant when compared to control
Subject(s)
Animals, Laboratory , Liver/drug effects , Rats , AloeABSTRACT
Oxidative damage has been suggested to be a contributory factor in the development and complications of atherosclerosis, and of recent the beneficial effects of antioxidants against some pathologies have gained considerable interest. We evaluated the protective effects of flavonoids from Garcinia kola seeds on the oxidation of human low- density lipoprotein [LDL] and their ability to scavenge reactive oxygen species [ROS] in vitro. Department of internal medicine, Faculty of Medicine and Health Sciences, UAE University, Al Ain, UAE. Human low- density lipoprotein [LDL] was prepared from fresh blood donated by volunteers with informed consent. The inhibitory effect of varying concentrations of flavonoids from Garcinia kola seeds, namely; Kolaviron [KV], Garcinia biflavanone [GB] 1, GB 2 and kolaflavanone [KF] on Fe/ ascorbate- induced peroxidation in LDL was investigated. Likewise the scavenging effect of these flavonoids on superoxide radicals and hydrogen peroxide in vitro was examined. In the present study, KV, GB1, GB2 and KF exhibited marked scavenging effect [41- 93%] on H[2]O[2], in vitro and the scavenging activities of these flavonoids were concentration dependent. Furthermore, KV, GB1, GB2 and KF showed marked activity as superoxide radicals [O[2] .] scavenger. At 1 mg/ ml, KV, GB1, GB2 and KF scavenged O[2] . by 71%, 52%, 66% and 48%, respectively. In addition, KV, GB1, GB2 and KF showed marked reducing property on potassium ferricyanide in vitro. KV, GB1 and GB2 were effective at preventing LDL lipid peroxidation [LPO] induced by Fe/ ascorbate system. Specifically at 0.01 mM, 0.1 mM and 1.0 mM, KV inhibited LPO by 29%, 61% and 79%, while GB1 inhibited LPO by 29%, 56% and 86%, respectively. At these concentrations, GB2 reduced LPO by 41%, 72% and 91%, respectively. However, the inhibition of LDL peroxidation by KV, GB1 and GB2 were concentrations dependent. KF showed significant [p<0.05] inhibition of LDL peroxidation at 1.0 mM only. The inhibition of LDL peroxidation by these flavonoids follow the trend GB2 > GB1 > KV > KF at 1.0 mM. These flavonoids are active scavengers of H[2]O[2] and O[2] . radicals in vitro and their antilipoperoxidative effect might be linked to free radical scavenging ability, and this could be a pointer to their possible anti- atherogenic property