Chemical composition of proteins and polysaccharides produced by semi-solid fermentation

Comparative studies on mango and date by-products in semisolid fermentation with Pichia pinus were achieved. Optimal cultural conditions on both by-products were 30 +/- 1C, pH 5.1-5.5 and aeration at 20 L/min./kg. The maximum biomass and protein contents of mango and date cultures were 66.6 and 56.1 g/kg dry substances and 36.6 and 38.6 g/100 g cell biomass, respectively. Two protein fractions were precipitated at pH 8.0 and 3.5 from the extract previously obtained at pH 12.0. The amino acids profile of the different protein fractions revealed the presence of all common amino acids and substantial amounts of essential amino acids. Paper chromatographic analyses of hydrolysates of total, soluble and insoluble non-cellulosic polysaccharides [NCP] indicated the presence of substantial amounts of mannose, rhamnose and ribose after 60 hours of fermentation which were not found at zero time when using both by- products

Sugar composition of extra and intracellular polysaccharides produced by candida lipolytica [4124] grown on methanol media

Candida lipolytica [4124] was grown on different methanol culture conditions for single cell protein production. The extracellular polysaccharides amount produced in 3 different methanol media was found to be ranging between 141.0 and 185.0mg/L at the 3rd day of growth. The free monosaccharides of Candida lipolytica [4124] cells were identified and estimated. The chromatographic analysis of the hydrolysates of intracellular polysaccharides indicated the presence of glucose, galactose and mannose as dominant bound sugars; xylose was present only in media containing 2,6-dichlorophenol indophenol