ABSTRACT
Objective/background: Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t[8;14][q24;q32], which involves the c-myc gene [8q24] and the immunoglobulin heavy-chain [IgH] locus [14q32], accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma [DLBCL]. Long-distance polymerase chain reaction [LD-PCR] assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t[8;14] c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL
Methods: In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene [joining region and c switch region] and the myc gene [Exons 2 and 3]
Results: As much as 1.85% of the samples were positive for the c constant region and Exon 2 of the myc gene
Conclusion: LD-PCR can be used for the detection of t[8;14] c-myc/IgH gene rearrangement in patients with DLBCL
ABSTRACT
Human papilloma virus causes benign and malignant abnormalities in different part of the body. The link between high risk types of HPV and some anogenital and aerodigestive tract cancer is well established. Oral HPV infection plays a role in developing oropharyngeal squamous cell carcinoma. We studied the prevalence of oral HPV in healthy individuals and its relative risk factors. Saliva samples of 114 healthy subjects were collected for HPV DNA analysis. Volunteers completed questionnaires and signed a written consent. For data analysis descriptive statistic, chi square test and odds ratio was used. The frequency of oral HPV in healthy individuals was 6.1% [seven participant]. The most frequent type was HPV-18 in five of them.HPV-6 and HPV-66 each was detected in one case. Relation of oral HPV positivity to demographic features and risk factors was not statistically significant. The prevalence of oral HPV infection in our community is the same as many other communities of developing countries, stressing that HPV-18 were the dominant type
Subject(s)
Humans , Female , Male , Risk Factors , Prevalence , Saliva , Mouth NeoplasmsABSTRACT
Peroxisome proliferative-activated receptors [PPARs] are nuclear receptors that involved in cellular lipid metabolism and differentiation. The subtype gamma of the PPAR family [PPAR gamma] plays important roles in physiologic functions of ovaries. To determine correlation between PPAR gamma protein level in granulosa cells and pregnancy rate in women undergoing in-vitro fertilization [IVF] treatment. In this cross-sectional study, twenty-five samples of granulosa cells were collected from women referred to an IVF treatment center. PPAR gamma protein expression level in granulosa cells was determined in comparison with beta -actin level as control gene with Western blot test. Laboratory pregnancy was determined by a rise in blood beta -hCG level fourteen days after embryo transfer. Correlation analyses were used to test for associations between the oocytes and pregnancy occurrence as outcome variables and PPAR gamma protein expression level. Correlation analysis indicated that there was no significant relationship between granulosa cells PPAR gamma protein level with IVF parameters including number of matured oocytes and the ratio of fertilized to matured oocytes. Comparison of granulosa cells PPAR gamma protein level with positive and negative laboratory pregnancy revealed also no significant relationship. According to the results of this study, PPAR gamma protein level in granulosa cells could not be directly correlated to the success rate of IVF