ABSTRACT
In order to selected indigenous potential probiotic bacteria, we surveyed antagonistic activities of 22 strains of acid and bile toleran Lactobacillus, isolated from traditional dairy products by biochemical and molecular methods. In a fundamental practical study assessment of antimicrobial activity of this strain with neutralized and Dual layer two methods against bacterial pathogene such as E-coli, L. monocytogenes, S.auteus and Y.entercolitica was done. These strain were identified with two methods for determining of biochemical and sequence of 16 SrDNA. Dual layer method based on the growth of zone diameter were established in three groups of strains; inhibitors, semi inhibitors and non inhibitors. Neutralize method around well acidic extract containing strains C5i4, Y144, K213, C4ie, C612 and neutral extract C5i4 zone blight strains was observed. Based on the results, sequence area 16Sr DNA of four strains include C4i2, D1d2, Y2c4, D3b1 indicator bacteria that revealed the highest percentage of inhibitor effect of bacterial indicators, were duplicate and sequency. So four strains L.Bacilus Pentosus, L.Bacillus Bervis and L. Bacillus Paraplantarum, were identified respectively. It seems that indigenous lactobacillus from Iranian dairy products have potential as probiotics. So use of them as bio preservative prevent food bacterial contamination