ABSTRACT
BACKGROUND@#Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. @*METHODS@#An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 x 106 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n= 14). The DHP scaffold, 1 x 106 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. @*Results@#The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p 0.05). @*Conclusion@#In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
ABSTRACT
BACKGROUND@#Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. @*METHODS@#An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 x 106 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n= 14). The DHP scaffold, 1 x 106 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. @*Results@#The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p 0.05). @*Conclusion@#In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
ABSTRACT
To evaluate the results of surgical drainage and use of synthetic glue in Morel-Lavallee lesions. We treated 7 Morel-Lavallee lesions in Ankara Bayindir Hospital, Ankara, Turkey between April 2003 and June 2004. These lesions developed in 5 male patients due to crush under a vehicle or a traffic accident. The mean age was 32.8 years; range 16-55. All lesions were localized in thigh. One patient had surgery due to acetabulum fracture and one had an urological operation for urethral rupture. All patients were operated 6-48 hours after the injury. After surgical drainage, soft tissues were attached with the use of synthetic glue and compressive bandage was applied. We defined healing as the loss of fluctuation and elicitation of the normal mobility of the injured skin on manual examination. The mean follow-up was 11.6 months [range 8-20 months]. One patient was operated for acetabulum fracture and had a bilateral Morel-Lavallee lesion 2 days after the operation. This patient was reoperated for drainage. All lesions were healed with a mean of 5 weeks [range 3-8 weeks]. No infection, necrosis or recurrences were detected during the follow-up. Results of the treatment with the use of synthetic glue and surgical drainage in Morel-Lavallee lesion are satisfactory with early healing time without recurrence