ABSTRACT
Aim: The aim of this study was to compare the cytotoxic effects of two indirect composite resins (Artglass and Solidex) on the viability of L-929 fibroblast cells at different incubation periods by storing them in artificial saliva (AS). Materials and Methods: Disk-shaped test samples were prepared according to manufacturers' instructions. Test materials were cured with light source (Dentacolor XS, Heraus Kulzer, Germany). The samples were divided into two groups. The first group's samples were transferred into a culture medium for 1 hour, 24 hours, 72 hours, 1 week and 2 weeks. The other group's samples were transferred into a culture medium for 1 hours, 24 hours, 72 hours, 1 week, and 2 weeks after being stored in AS for 48 hours. The eluates were obtained and pipetted for evaluation onto L-929 mouse fibroblast cultures incubated for 24 hours. Measurements were performed by MTT (3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The degree of cytotoxicity for each sample was determined according to the reference values represented by the cells with a control group. Results: Statistical significance was determined by ANOVA. Both groups presented lower cell viability in comparison to the control group at all periods. Storing in artificial saliva reduced cytotoxicity significantly (P < 0.05). Stored Artglass and Solidex showed similar effects on cytotoxicity. Nonstored Solidex samples were found more cytotoxic than Artglass samples. The cell survival rate results of 24-hour incubation period were significantly lower than those of the other experimental periods (P < 0.05). Conclusion: Storing indirect composite resins in AS may reduce cytotoxic effects on the fibroblast cells. However, resin-based dental materials continue to release sufficient components to cause cytotoxic effects in vitro after 48 hours of storing in AS.
ABSTRACT
OBJECTIVE: Applications of resin luting agents and high-power light-emitting diodes (LED) light-curing units (LCUs) have increased considerably over the last few years. However, it is not clear whether the effect of reduced exposure time on cytotoxicity of such products have adequate biocompatibility to meet clinical success. This study aimed at assessing the effect of reduced curing time of five resin luting cements (RLCs) polymerized by high-power LED curing unit on the viability of a cell of L-929 fibroblast cells. MATERIAL AND METHODS: Disc-shaped samples were prepared in polytetrafluoroethylene moulds with cylindrical cavities. The samples were irradiated from the top through the ceramic discs and acetate strips using LED LCU for 20 s (50 percent of the manufacturer's recommended exposure time) and 40 s (100 percent exposure time). After curing, the samples were transferred into a culture medium for 24 h. The eluates were obtained and pipetted onto L-929 fibroblast cultures (3x10(4) per well) and incubated for evaluating after 24 h. Measurements were performed by dimethylthiazol diphenyltetrazolium assay. Statistical significance was determined by two-way ANOVA and two independent samples were compared by t-test. RESULTS: Results showed that eluates of most of the materials polymerized for 20 s (except Rely X Unicem and Illusion) reduced to a higher extent cell viability compared to samples of the same materials polymerized for 40 s. Illusion exhibited the least cytotoxicity for 20 s exposure time compared to the control (culture without samples) followed by Rely X Unicem and Rely X ARC (90.81 percent, 88.90 percent, and 83.11 percent, respectively). For Rely X ARC, Duolink and Lute-It 40 s exposure time was better (t=-1.262 p=0,276; t=-9.399 p=0.001; and t=-20.418 p<0.001, respectively). CONCLUSION: The results of this study suggest that reduction of curing time significantly enhances the cytotoxicity of the studied resin cement materials, therefore compromising their clinical performance.