ABSTRACT
The survival and surface adherence ability of Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa in nutrient broth and in five oral liquid pharmaceuticals (nivaquine syrup, cough mixture, paracetamol elixir, cotrimoxazole and vitamin C) were investigated The bacteria grew more in nutrient broth than in the pharmaceuticals (p < 0. 001) and the recovery of stressed cells was enhanced when 3 Tween 80 was used as the recovery medium as against the use of normal saline (p < 0.01). The Gram-negative bacteria were more adapted to the pharmaceuticals than their Gram-positive counterparts. Klebsiella pneumoniae and Ps. aeruginosa were recovered in large numbers from nivaquine and cotrimoxazole suspensions that did not support the growth of the other bacteria. The effect of bacterial growth on the physico-chemical properties of the pharmaceuticals was also evaluated The properties were not altered significantly except for pH shifts of 0.3 to 1.1 caused by E. coli and S. aureus in paracetamol and vitamin C. Adherence capability was found to correlate with the survival ability of the bacteria. Populations on coupons were significantly higher when nutrient broth was used as the suspending medium compared with any of the pharmaceuticals (p < 0.01). Rubber and plastic coupons were significantly more accessible to the bacteria than glass coupon as revealed by the high population of bacteria recovered from their surfaces
Subject(s)
Humans , Gram-Negative Bacteria/physiology , Gram-Positive Bacteria/physiology , Drug Contamination , Bacterial Physiological Phenomena , Adhesiveness , Administration, Oral , Gram-Negative Bacteria/isolation & purification , Gram-Negative Bacteria/pathogenicity , Gram-Positive Bacteria/isolation & purification , Gram-Positive Bacteria/pathogenicity , Colony Count, Microbial , Culture MediaABSTRACT
OBJETIVO: Avaliar o impacto do planejamento urbano, da infra-estrutura, do saneamento e dos índices pluviométricos sobre a qualidade bacteriológica do abastecimento domiciliar de água. MÉTODOS: Foi realizada cultura de amostras de água obtida de poços superficiais e profundos, fossos e água corrente de bicas públicas para determinar o número mais provável de Escherichia coli e coliformes totais por meio da técnica de múltiplos tubos. Patógenos entéricos foram detectados pelo uso de meios diferenciais e seletivos. Amostras foram coletadas durante os períodos de seca e de chuvas intensas em municípios com características singulares de infra-estrutura, planejamento urbano e saneamento. RESULTADOS: A contaminaçäo de água tratada ou encanada esteve relacionada à distância do ponto de coleta com relaçäo à estaçäo de tratamento. Defeitos na canalizaçäo aumentaram o índice de contaminaçäo (p<0,5), principalmente em áreas densamente povoadas com infra-estrutura arruinada. Os despejos de bueiros representaram a principal fonte de contaminaçäo da água encanada. Houve maior contaminaçäo em poços superficiais do que em fossos e durante os períodos de chuvas intensas (p<0,05). E. coli e outros patógenos entéricos foram isolados de fontes de abastecimento contaminadas. CONCLUSOES: A falta de planejamento urbano, as más condiçöes de infra-estrutura e a localizaçäo indiscriminada de poços e fossos contribuíram para a baixa qualidade bacteriológica do abastecimento domiciliar de água. As águas das chuvas agravaram o impacto