ABSTRACT
Objective: was to evaluate the retentive force, fatigue resistance and deformity of clasps made from two materials cobalt chromium and poly ether ether ketone (PEEK). Material and Methods: sixteen models were fabricated,each one having lower 1st molar. Models were divided into two groups according to materials,group I (GI) for cobalt chromium (Co-Cr) and group II (GII) for PEEK. Each testing models and its clasps were mounted inside universal testing machine, the retention was measured by applying withdrawal force to it by this machine at 5 mm/min. The Fatigue resistance is measured by the reduction in retention through repeated insertion and removal cycles using robota chewing simulator. Removal and insertion cycling of clasps was carried out for 360, 730, 1080, 1,440, 2,116 and 2,880 cycles (corresponding to 3, 6, 9, 12, 18 and 24 months of simulated clinical use of a RPD) to simulate the fatigue resistance test. Deformity was measured before and after each cycling using a digital micrometer. Statistical analysis was done by 2-way-ANOVA test to detect significance effects of each variable. Results: Retention and fatigue resistance, after 360, 730, 1080, 1,440, 2,116 and 2,880 cycles totally the difference between Co-Cr and PEEK groups was statistically non-significant (P = 0.0980> 0.05) where (Co-Cr > PEEK). Deformation results, regardless to evaluation time, Co-Cr group recorded statistically significant higher deformation mean value than PEEK group (P = 0.008 < 0.05).Regardless to material group, deformation mean value changed nonsignificantly by time (P = 0.2882> 0.05). Conclusion: PEEK clasps (1.0 mm in cross section diameter) engage 0.50 mm undercut provide sufficient retention nearly similar that of Co-Cr clasps.(AU)
Objetivo: o objetivo foi avaliar a força de retenção, a resistência à fadiga e a deformidade dos grampos fabricados com dois materiais diferentes: cromo-cobalto (Co-Cr) e poliéter-éter-étercetona (PEEK). Materiais e métodos: dezesseis modelos foram confeccionados, cada um com 1º molar inferior. Os modelos foram divididos em dois grupos de acordo com o material: grupo I Co-Cr e grupo II para PEEK. Cada modelo de teste universal e seus grampos foram montados em uma máquina de teste universal. A retenção foi medida aplicando força de retirada por esta máquina a 5 mm/min. A resistência à fadiga foi medida pela redução na retenção através de ciclos repetidos de inserção e remoção usando um simulador do ciclo de mastigação. O ciclo de remoção e inserção dos grampos foi realizado por 360, 730, 1080, 1.440, 2.116 e 2.880 ciclos (correspondendo a 3, 6, 9, 12, 18 e 24 meses de uso clínico simulado de um RPD) para simular o teste de resistência à fadiga. A deformidade foi medida antes e após cada ciclo, usando um micrômetro digital. A análise estatística foi realizada pelo teste ANOVA de 2 fatores para detectar os efeitos de significância de cada variável. Resultados: A retenção e a resistência à fadiga após 360, 730, 1080, 1.440, 2.116 e 2.880 ciclos entre os grupos Co-Cr e PEEK não mostrou diferença estatisticamente significativa (p = 0,0980> 0,05), onde (Co-Cr> PEEK). Para os resultados da deformação, independentemente do tempo de avaliação, o grupo Co-Cr registrou um valor médio de deformação maior, com diferença estatística significativa, quando comparado com o grupo PEEK (p= 0,008 < 0,05). Independentemente do grupo de material, o valor médio da deformação foi alterado de forma não significativa pelo tempo (p= 0,2882> 0,05). Conclusão: os grampos PEEK (1,0 mm de diâmetro da seção transversal) engatam 0,50 mm retenção quase semelhante à dos grampos Co-Cr (AU)
Subject(s)
Resins, Synthetic , Chromium Alloys , Denture, PartialABSTRACT
Objective: To develop a new sandwich based lateral flow immunochromatographic strip for rapid detection of circulating Schistosoma mansoni antigen in serum and urine samples of patients with active schistosomiasis. Methods: This lateral flow immunochromatographic strip was prepared by using anti-Schistosoma mansoni soluble egg antigen monoclonal antibody conjugated gold nanoparticles (MAb-AuNPs) as antigen-detecting antibody, while crystalline material (MCM)-41-MAb bioconjugate was immobilized at the test line as antigen-capturing antibody. Both antigen capturing and detecting antibodies formed sandwich complexes with circulating Schistosoma mansoni antigen in the positive samples. Sandwich complexes immobilized at the test line gave distinct red color. The assay reliability was examined by using urine and serum samples of 60 Schistosoma mansoni infected patients, 20 patients infected with parasites other than Schistosoma, and 20 healthy individuals as negative controls. Results were compared with those obtained via sandwich enzyme linked immunosorbent assay (ELISA). Results: The detection limit of circulating Schistosoma mansoni antigen by lateral flow immunochromatographic strip was lower (3 ng/mL) than the detection limit by ELISA (30 ng/mL). The sensitivity and specificity of lateral flow immunochromatographic strip in urine samples were 98.3% and 97.5%, respectively compared to 93.5% and 90.0% by ELISA. In serum samples, they were 100.0% and 97.5%, respectively compared to 97.0% and 95.0% by ELISA. The strip test took approximately 10 min to complete. Conclusions: This new lateral flow immunochromatographic strip offers a sensitive, rapid, and field applicable technique for diagnosis of active schistosomiasis.
ABSTRACT
Background: Speckle-tracking echocardiography has emerged as a unique technique for accurately evaluating myocardial function by analyzing the motion of speckles identified. Speckle-tracking measured under stress may offer an opportunity to improve the detection of dynamic regional abnormalities and myocardial viability. Objective: The aim of the current study was to evaluate stress speckle tracking to detect myocardial viability in comparison to cardiac MRI in post-STEMI patients. Patients and methods: 74 patients were prospectively enrolled in 18-month's study. Dobutamine stress echocardiography was performed 4 days post-infarction accompanied with automated functional imaging analysis of left ventricle during rest and then during low dose stress. All patients underwent a follow up stress echocardiography at 3 months with speckle tracking analysis. Cardiac MRI took place concomitantly at 4 days post-infarction and 3 months. Results: Investigating strain rate obtained with stress speckle tracking after revascularization predicted the extent of myocardial scar, determined by contrast-enhanced magnetic resonance imaging. A good correlation was found between the global strain and total infarct size (R 0.75, p< 0.001). Furthermore, a clear inverse relationship was found between the segmental strain and the transmural extent of infarction in each segment. Meanwhile it provided 81.82% sensitivity and 82.6% specificity to detect transmural from non-transmural infarction at a cut-off value of -10.15. Conclusion: Strain rate obtained from speckle tracking during stress is a novel method of detecting myocardial viability after STEMI. Moreover, it carries a promising role in post-myocardial infarction risk stratification with a reasonable prediction of reversible cardiac-related hospital re-admission
Subject(s)
Echocardiography, Stress , Egypt , Magnetic Resonance Imaging , Myocardial Infarction/complications , Myocardial Infarction/diagnostic imagingABSTRACT
Objective: To investigate the possible protective and/or therapeutic potentials of Dunaliella salina (D. salina) biomass, its carotenoid and polar fractions on cardiac dysfunction associated with D-galactose (D-GAL) induced aging in rats. Methods: Aging associated cardiac dysfunction was induced in rats by injection of D-GAL (200 mg/kg; i.p) for 8 weeks. D-GAL injected rats were treated with two regimens; protective regimen where D. salina biomass (250 mg/kg), its carotenoid (250 μg/kg) and polar (250 μg/kg) fractions were given orally for two weeks concurrently with D-GAL injection as well as treatment regimen where the three treatments were given orally for 28 consecutive days after D-GAL injection. Results: D-GAL injection for 8 weeks was accompanied with dramatic electrocardiographic changes as well as profound elevation in serum levels of homocysteine, creatinine kinase isoenzyme and lactate dehydrogenase in addition to the reduction of the cardiac content of glucose trasporter 4. D-GAL also induced reduction in cardiac superoxide dismutase activity and elevation of inducible nitric oxide synthetase and interleukin-6. On the other hand, oral administration of D. salina carotenoid fraction as well as the total biomass significantly attenuated the D-GAL-induced disturbances in the above mentioned parameters where the protective regimen appeared more successful in controlling the manifestations of cardiac dysfunction. The histopathological examination further emphasized the promising results. Besides, the HPLC analysis of the carotenoid fraction of D. salina revealed the presence of 2.31％ β-carotene. Conclusions: D. salina carotenoid fraction as well as the total biomass ameliorate D-GAL-induced aging associated cardiac dysfunction which is attributed to the potent antioxidant activity of β-carotene.
ABSTRACT
Objective: To investigate the possible protective and/or therapeutic potentials of Dunaliella salina (D. salina) biomass, its carotenoid and polar fractions on cardiac dysfunction associated with D-galactose (D-GAL) induced aging in rats. Methods: Aging associated cardiac dysfunction was induced in rats by injection of D-GAL (200 mg/kg; i.p) for 8 weeks. D-GAL injected rats were treated with two regimens; protective regimen where D. salina biomass (250 mg/kg), its carotenoid (250 μg/kg) and polar (250 μg/kg) fractions were given orally for two weeks concurrently with D-GAL injection as well as treatment regimen where the three treatments were given orally for 28 consecutive days after D-GAL injection. Results: D-GAL injection for 8 weeks was accompanied with dramatic electrocardiographic changes as well as profound elevation in serum levels of homocysteine, creatinine kinase isoenzyme and lactate dehydrogenase in addition to the reduction of the cardiac content of glucose trasporter 4. D-GAL also induced reduction in cardiac superoxide dismutase activity and elevation of inducible nitric oxide synthetase and interleukin-6. On the other hand, oral administration of D. salina carotenoid fraction as well as the total biomass significantly attenuated the D-GAL-induced disturbances in the above mentioned parameters where the protective regimen appeared more successful in controlling the manifestations of cardiac dysfunction. The histopathological examination further emphasized the promising results. Besides, the HPLC analysis of the carotenoid fraction of D. salina revealed the presence of 2.31% β -carotene. Conclusions: D. salina carotenoid fraction as well as the total biomass ameliorate D-GAL-induced aging associated cardiac dysfunction which is attributed to the potent antioxidant activity of β -carotene.
ABSTRACT
Background: Autism is a behaviorally defined neurodevelopmental disorder of unknown etiology
Objective: To assess serum copper and ceruloplasmin levels in Egyptian autistic children patients
Subjects and methods: 40 participants have been subjected to thorough history taking, complete clinical examination, IQ assessment, estimation of serum copper and ceruloplasmin levels
Results: A statistically significant difference was found between patients and controls as regards stereotypic movements, absent eye contact, delayed motor development, delayed speech and IQ [p < 0.01 for each item]. Mean level of copper was significantly higher in patients than in controls [P < 0.001], also mean level of ceruloplasmin was significantly higher in patients than controls [P = 0.009]
Conclusion: Serum copper level may have a role in the pathogenesis of autism
ABSTRACT
Background: Major depressive disorder [MDD] is a common brain disorder that affects approximately 10% of the world population and leads to significant disability. The current study was aimed to evaluate the impact of Omega3 PUFAs administration on the clinical outcome of patients with depression
Patients and Methods: This prospective, randomized controlled study included a total of Forty-two patients who diagnosed with depression according to a strict inclusion and exclusion criteria, attending at the outpatient clinics of the Department of Psychiatry, Al-Zahraa University Hospital, Cairo, Egypt. Approval of the Ethical Research Committee of Faculty of Pharmacy, Ain Shams University [registration number 61], and a written informed consent from all the subjects were obtained. This study was conducted between February 2015 till August 2016. patients were randomly assigned to either; Group1; [intervention n=21]; received the prescribed antidepressant + omega3 [2100mg] for 8 weeks, or Group 2; [control, n=21]; received the prescribed antidepressant only for 8 weeks. Baseline evaluation and 8-week assessment included; patient demographicdata collection, history taking and clinical assessment of DSM-5 criteria and HAM-D score. Laboratory assessment included; complete blood picture [CBC], prothrombin time [PT], activated partial thromboplastin time [aPTT]. Patients were followed up regularly every 2 weeks for 8 weeks for the occurrence of side effects due to antidepressants/ Omega 3 and compliance with medications
Results: The 2 groups were comparable at baseline. The test group showed a significant improvement in the HAMD score from baseline values and versus the control. There was no significant difference in the reported side effects between the 2 groups
Conclusion: Omega -3 PUFAs administration at a dose of 2100 [EPA1350 mg/, DHA 600 mg/ 150 mg other omega 3 FA] for 8 weeks, improved depression symptoms and was well tolerated
ABSTRACT
Background: pregnancy represents an important challenge in the female's life that she needs close observation during it and also needs counseling by her physician about the changes that are going through her body in this stressful period
Aim of the Work: the purpose of our study was to detect the changes that occur in the women's eyes during pregnancy and postpartum period regarding intraocular pressure and refraction
Patients and Methods: we studied 40 pregnant healthy women whose ages ranged from 18 to 35 years and we exclude any ocular diseases such as keratoconus, amblyopia or diabetic retinopathy. We measured the intraocular pressure using a Goldmann applanation tonometer, best corrected visual acuity using the Snellen's chart and k reading using autorefractor-keratometer
Results: we found that the IOP decreases only in the 3rd trimester with more decrease in women with multiple fetuses and multiple pregnancies. We found that the BCVA decreases only in the 2nd and 3rd trimesters. We also found that the K reading increases only in the 2nd and 3rd trimesters. Intraocular pressure, best corrected visual acuity and k reading returned to the normal values after delivery
Conclusion: the physiologic changes that occur in the women's eyes during pregnancy and postpartum period are usually marked in second and third trimester; this is because at this period, hormonal activity is at its peak, and however these changes are transient because several weeks postpartum, all hormonal activities return to their prenatal levels
ABSTRACT
Background: retinoblastoma is the most common primary, malignant intraocular tumor of childhood, the aim of the study is to evaluate the outcome of management of 50 consecutive cases of unilateral retinoblastoma
Aims of the Work: to evaluate the outcome of management of unilateral retinoblastoma and to assess the risk of recurrence
Patients and Methods: prospective study of 50 consecutive cases with unilateral intraocular retinoblastoma. The management of cases was performed in the National eye center [Rod El Farag]. Fundus examination was done under general anesthesia with RetCam 3. Management of cases according to the staging was group A: Focal treatment, TTT or cryotherapy, group B and C: chemotherapy with focal treatment, group D: If the patient has good visual potential [according to macular affection] Chemotherapy with focal treatment, if the patient has poor visual potential Enucleation, group E Enucleation
Results: as regard cases that managed with chemotherapy, there was 23 cases, 13 out of them treated with chemotherapy and transpupillary thermotherapy and showed complete regression, 10 cases showed initial regression with further new tumor recurrence after finishing chemotherapy, 5 cases was well managed by focal therapy, and another 5 cases failed to be controlled by focal therapy and Intra vitreal melphalan and ended by enucleation. As regard cases that was managed by enucleation, there was 32 cases, 27 out of them managed by primary enucleation, and 5 cases managed by secondary enucleation after failure of chemotherapy and focal therapy, 17 cases out of the enucleated eyes revealed presence of high risk pathological features, none of the cases developed local or metastatic tumors in the follow up period
Conclusion: retinal seeding, vitreous seeding are major predictive factors for tumor recurrence
ABSTRACT
Background: Melanocortinergic system represents a known system involved in the central regulation of body weight with the central proopiomelanocortin [POMC] neurons forming a potent anorexigenic network. Polymorphisms in the POMC gene locus are associated with obesity phenotypes
Aim: To assess the contribution of the POMC gene 9-bp insertional polymorphism in the susceptibility to obesity and its relation to body mass index [BMI] and adiposity-related co-morbidities in obese children and adolescents; as well as binge eating behavior
Patients and methods: Fifty obese children and adolescents with simple obesity were screened for Binge Eating Disorder [BED] by The Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition [DSM-5], they were compared to 50 age, sex and pubertal stage-matched non obese controls. Anthropometric measurements, blood pressure, abdominal ultrasound for fatty liver, measurement of fasting lipid profile, fasting insulin, fasting blood glucose and assessment of POMC gene 9-bp insertional polymorphism were done
Results: Obese patients had significantly higher anthropometric measurements, blood pressure percentiles, fasting glucose, fasting insulin, homeostasis model assessment for insulin resistance [HOMA-IR] and fasting lipid profiles, and higher frequency of occurrence of non alcoholic fatty liver disease and BED. Allelic frequencies of POMC gene 9 bp insertional polymorphism were comparable in patients and controls [p= 0.956]. Fasting insulin levels were significantly higher in the heterozygous cases having the polymorphism than in wild homozygous cases; whereas no difference was observed among the controls
Conclusion: This polymorphism was associated with higher fasting insulin levels in the obese patients only. These findings support the hypothesis that the melanocortin pathway may modulate glucose metabolism in obese subjects indicating a possible gene-environment interaction. POMC variant may be involved in the natural history of polygenic obesity, contributing to the link between type 2 diabetes and obesity
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Pro-Opiomelanocortin/genetics , Polymorphism, Genetic , Feeding Behavior , Body Mass Index , Adiposity , Child , Adolescent , Metabolism , Case-Control StudiesABSTRACT
Background: Autism is a neurodevelopmental disorder characterized by clinical, etiologic and genetic heterogeneity. Many surveys revealed cytogenetically visible chromosomal abnormalities in 7.4% of autistic patients documented as well as several submicroscopic variants. This study had been conducted to identify some aspects that might be involved in the pathogenesis of autism which is necessary for offering proper genetic counseling to families of autistic patients and their role in the prenatal diagnosis of autism
Methods: This cross sectional study was conducted at the Child Psychiatry Clinic, Pediatric Hospital, Ain Shams University on 30 autistic patients who were subjected to the following tools: Confirmation of diagnosis using DSM-IV-TR criteria, IQ assessment using Stanford-Binet intelligence scale and assessment of severity of autistic symptoms using childhood autism rating scale [CARS]. Full clinical examination, neurological examination, EEG, audiological assessment were also done. High resolution karyotyping was done for detection of numerical or structural chromosomal abnormalities as deletion, duplication, translocation of chromosomes
Results: All the results of cytogenetic analysis were normal with no detectable numerical or structural chromosomal abnormalities. Males are affected more than females, only one case had history of drug intake [progestin], two cases had history of anti-D injection and two cases had history of diabetes mellitus during pregnancy. Four cases had history of respiratory distress and seven cases had history of jaundice. Two cases had history of generalized tonic clonic convulsion and four cases had history of EEG abnormalities. Fifteen cases of our autistic patients had mild mental retardation and six cases had moderate mental retardation
Conclusion: Chromosomal abnormalities were not detected in the studied autistic children, and so the relation between the genetics and autism still needs further work up with different study methods and techniques
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Chromosome Aberrations , Cross-Sectional Studies , Karyotyping , KaryotypeABSTRACT
To identify polymorphism of Factor V Leiden and Prothrombin gene in women suffering from preeclampsia. From 142 pregnant women we identified 92 women suffering from preeclampsia and 50 healthy controls with normal pregnancy matched for age and socioeconomic status, preeclampsic patient classified as mild preeclampsia 42[45.7%] and severe preeclampsia 50[54.3%]. Blood samples were tested for DNA polymorphism affecting thrombophilia Factor V Leiden and Prothrombin gene polymorphism. Heterozygous AG genotype showed a significant high frequency among preeclampsic patients [20.7%] compared to controls [4.0%], [OR 6.2, P= 0.006] regarding to Prothrombin gene but: Factor V Leiden, AG genotype showed [8.7%] of preeclampsic patients which was absent in any of the controls
Subject(s)
Humans , Female , Pregnancy , Factor V , Prothrombin , Polymorphism, GeneticABSTRACT
To identify polymorphism of methylenetetrahydrofolate reductase gene in women suffering from preeclampsia. From 142 pregnant women we identify 92 women suffer from preeclampsia and 50 healthy controls with normal pregnancy matched for age and socioeconomic status, preeclampsic patient classified as mild preeclampsia 42[45.7%] and severe preeclampsia 50[54.3%]. Blood samples were tested for DNA polymorphism affecting thrombophilia methylenetetrahydrofolate reductase C677T and A1298C. Homozygous TT genotype, T allele of C677T polymorphism has a significantly higher frequency among preeclampsic cases compared to healthy controls [OR=21.7, 1.46, respectively]. Thus TT genotype and T allele may be considered as genetic risk factors for preeclampsic cases. On the other hand, non significant association in either genotype among preeclampsic cases compared to controls regarding to A1298C
Subject(s)
Humans , Female , Pregnancy , Polymorphism, GeneticABSTRACT
Numerous studies of autistic individuals have revealed evidence of cerebral hypoperfusion, neuroinflammation, gastrointestinal inflammation, immune dysregulation, oxidative stress, relative mitochondrial dysfunction, and neurotransmitter abnormalities. Many of these findings have been correlated with core autistic symptoms. For example, cerebral hypoperfusion in autistic children has been correlated with repetitive, self-stimulatory and stereotypical behaviors, and impairments in communication. Specifically, hyperbaric oxygen therapy [HBOT] has been used and can compensate for decreased blood flow by increasing the oxygen content of plasma and body tissues. The aim of this work was to study the effect of hyperbaric oxygen therapy in autistic Egyptian children. This prospective clinical trial study was conducted on 20 children diagnosed as autism based on DSM-IV-TR criteria [diagnostic and statistical manual of mental disorders, 4th edition criteria, text revised]. All patients received at least 20 sessions of hyperbaric oxygen therapy. Sessions were done at pressure 1.5 ATA [atmosphere absolute] with 100% oxygen concentration each lasting for 1-1.5 h either in multiplace chamber or monoplace chamber. MRI Perfusion of the brain was done before and after at least 20 HBOT sessions only for 6 cases. There was a statistically significant increase in the ratio of regional cerebral blood flow [RCBF] to white matter after HBOT in different brain regions when compared to their levels before HBOT. HBOT is a treatment that has recently become quite popular in the autism spectrum disorder [ASD] community. Its benefits cross a wide range of autistic traits as: improved language, increased awareness, behavior and socialization by affecting the pathophysiological findings in autism
Subject(s)
Humans , Male , Female , Hyperbaric Oxygenation , Child , Magnetic Resonance ImagingABSTRACT
THEBERA is a project funded by the European Union [EU], as an ERA-WIDE FP7 project, aiming to strengthen the Theodor Bilharz Research Institute [TBRI] capacities. A SWOT [strength/weakness/opportunities/threats] analysis of human, structural and organisational existing resources was performed in light of an extensive analysis of liver disease research and clinical management in Egypt, for a full understanding of TBRI needs. Strength and weakness features were identified and analysed, so were actions to be implemented and targets to be accomplished, to develop a business plan gathering the required critical mass [political, scientific, industrial, social] to select investment priorities, to sacrifice non-strategic areas of research, to promote national and international connections and industrial innovations, to update diagnostics and research device technologies and clinical management processes at European levels, to implement fundraising activities, to organise and properly assess training activities for young researchers, physicians, nurses, and technicians. Research institute self assessment is a priority need for sustainable capacity building and for future build-up of a competent health care research institute. Sustainable capacity building strategies must be designed on needs assessment, involving salient requirements: clear strategy, leverage of administrative capacities, industrial support and connections, systematised training programmes and enhancement of mobility of health care staff implemented within ill-defined boundaries and continuously re-evaluated with multiple feedback loops in order to build a complex, adaptable and reliable system based on value
ABSTRACT
Spirulina platensis are filamentous, undifferentiated, non-toxigenic cyanobacteria that have been used as food since ancient times. There have been numerous studies on its antioxidant and antimicrobial actions. In this study antibacterial and antiviral effect of ethanol extract of Spirulina platensis were tested. The reduction of infectious viral units after treatment with ethanol extract of Spirulina platensis was tested. Non toxic doses of Spirulina platensis revealed 53.3%, 66.7%, 76.7%, 56.7%, and 50% reductions in vitro for infectious units of adenovirus type 7, Coxsackievirus B4, astrovirus type 1, rotavirus Wa strain, and adenovirus type 40 respectively. Using disc diffusion method to show the antibacterial effect of ethanol extract of Spirulina platensis against different bacterial strains (Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Enterococcus faecalis) in addition to Candida albicans, inhibition zones were observed with Enterococcus faecalis and Candida albicans.
ABSTRACT
Despite its frequency, the polycystic ovary syndrome [PCOS] is still a difficult diagnosis in endocrinology, gynecology, and reproductive medicine. The Rotterdam consensus conference proposed to include the ultrasonographic follicle count as a new diagnostic criterion. Unfortunately, its assessment does not offer sufficient reliability worldwide. To explore the possible roles of altered circulating androgens and anti-mullerian hormone among PCOS women regarding their body mass index and their outcomes after IVF. In this cross sectional study, 195 women with PCO were included, they were divided according to their body mass index [BMI <27 kg/m[2]] as obese PCOS [n=91] and overweight PCOS [BMI >/= 27 kg/m[2]] [n=104]. Serum levels of androgens [dehydroepiandrosterone sulfate [DHEAS], testosterone and androstenedione [A4]], and anti-mullerian hormone [AMH] were assessed and compared with the endocrine profile and cycles outcomes. AMH, A4, FSH, and TSH concentrations were significantly higher in obese than in overweight women [p<0.001]. Contrary, LH: FSH ratio values, E[2], PRL and DAHE-S levels were significantly lower in obese than in overweight women [p<0.0001]. Total oocyte retrieved, mature and fertilized oocyte were significantly higher in obese than in overweight women. Among pregnant obese PCOS women both AMH and A4 were significantly increased and DAHE-S was significantly decreased compared to pregnant overweight PCOS women. Obese PCOS women have a higher chance of getting pregnant over those categorized as overweight PCOS. Also, androgens and AMH levels recommended to be considered in IVF attributes among obese and overweight PCOS women
Subject(s)
Humans , Female , Sperm Injections, Intracytoplasmic , Anti-Mullerian Hormone , Cross-Sectional Studies , Androstenedione , Androgens , Ovulation Induction , Pregnancy , Obesity , Follicle Stimulating Hormone , ThyrotropinABSTRACT
A disintegrin and metalloproteinase-encoding gene [ADAM33], was recently identified as an asthma susceptibility gene. ADAM33 protein is expressed in smooth muscle cells of bronchi and pulmonary fibroblasts, playing a major role in airway remodeling. Earlier studies, have mostly confirmed a link between ADAM33 and asthma as well as bronchial hyperresponsiveness. This work studied a group of Egyptian asthmatic children for 3 ADAM33 single nucleotide polymorphisms [SNPs], previously identified as putative risk alleles: T1 G > A[rs2280091], T2 A > G[rs2280090], V4 G > C[rs2787094] using Polymerase Chain Reaction - restriction fragment length polymorphism [PCRRFLP] with emphasis on their relation to clinical [severity, smoking, family history, and atopic manifestations] and laboratory data [Ig Immunoglobulin E [Ig E] level and absolute eosinophilia] and pulmonary functions. Sixty [3-12 years old] asthmatic children and 32 matched controls were recruited. The genotype distribution for the SNPs showed no significant difference between the patients and the controls. A higher frequency of the [AA] genotype of T1 polymorphism was found in controls [75%] than in patients [41%], while the [AG] variant was higher in cases [46.6%] than in controls [21.9%] but with no statistically significant difference. Also the [GG] genotype was higher in cases [11.6%] than in controls [3.1%] but with no statistical significance. The allelic frequencies of T1 showed a higher [A] allele in controls [85.93%] than cases [65%] and higher [G] allele in cases [35%] than controls [14.06%], showing a high significant difference. No correlation was found between [T1, T2, and V4] and the demographic, clinical and laboratory parameters, except SNP T1 showing a positive correlation with Ig E level, and SNP V4 showing a positive correlation with passive smoking as a precipitating factor and borderline significance with absolute eosinophilia. In conclusion, no significant association was detected between these SNPs and asthma susceptibility in this study
Subject(s)
Humans , Male , Female , Disintegrins/blood , Polymorphism, Genetic , Child , Respiratory Function Tests , Immunoglobulin E/bloodABSTRACT
Fascioliasis is an important food- and water-borne parasitic zoonosis caused by liver flukes of genus Fasciola [Digenea: Fasciolidae] of worldwide distribution. In Egypt, fascioliasis was encountered in nearly all Egyptian Governorates, particularly in the Nile Delta and specifically in Dakahlia. All enrolled cases were subjected to complete history taking, clinical examination, routine investigations and abdominal ultrasonography. Stool analysis, IHA and ELISA were used for fascioliasis diagnosis. Rural areas showed highest prevalence of fascioliasis than urban areas, however, but without significance [x[2]= 0.042 and P= 0.837]. Regarding human fascioliasis in examined the centers, no statistically significant difference [x[2] =2.824 and P=0.243] was detected. Regarding gender variation, the difference was statistically insignificant [x[2]= 0.166 and P= 0.683]. The difference between the age groups was statistically insignificant [x[2]= 3.882 and P=0.274]. Clinically, 7 cases [35%] were asymptomatic and another 13 cases [65%] had different clinical pictures. Abdominal pain, anemia, eosinophilia, and tender hepatomegaly were seen in 70%, 80%, 70%, and 10%; respectively. Of them, 11 cases showed positive abdominal ultrasonographic findings suggestive of fascioliasis
Subject(s)
Humans , Male , Female , Prevalence , Rural Population , Fascioliasis/diagnosisABSTRACT
Adipose tissue secretes a large number of adipocytokines such as leptin, resistin, and adiponectin. Many of these hormones and cytokines are altered in obese individuals and may lead to disruption of the normal balance between cell proliferation, differentiation, and apoptosis. The aim of our work was to investigate the disturbance of secretion of adiponectin and resistin in de novo and relapsed acute lymphoblastic leukemia [ALL] in Egyptian children and determine whether adiponectin and resistin are implicated in increased risk relapse compared to healthy individuals. Measurements of adiponectin and resistin were performed at diagnosis, in 32 patients with de novo ALL aged 3 to 18 years [mean 9.8 y] and 19 children with relapsed ALL aged 5 to 17 [mean 9.9 yr]. 10 apparently healthy children with matched age and sex were used as controls. Mean adiponectin levels were low [P < 0.05], whereas mean resistin levels were high [P<0.05] at diagnosis and relapsed ALL [compared to healthy controls]. A significant decrease of adiponectin levels was observed in relapsed ALL compared to de novo ALL. In contrast resistin was significantly increased in relapsed ALL compared to de novo patients. Adiponectin in ALL subjects inversely correlated with resistin level [r = -0.51, P < 0.001]. Low adiponectin and high resistin level at diagnosis suggest their implication in ALL pathogenesis and may serve as potential clinically significant diagnostic markers to detect leukemic relapse