ABSTRACT
Background and Aims: Acinetobacter baumannii is regarded as an important nosocomial pathogen around the world, especially in the intensive care unit that today seems to be resistant to the most antibiotics. Therefore, this study aimed to trace classes 1, 2, and 3 integrin in the isolates resistant to Acinetobacter baumannii
Materials and Methods: In this descriptive study, Acinetobacter baumannii of 67 patients in Ahvaz hospitals were all isolated and their antibiotic resistance pattern was determined by the disk diffusion. The presence of genes coding for antibiotic resistance as well as integrons [class 1, 2 and 3] were analyzed using polymerase chain reaction method
Results: Out of 67 isolates, the most resistance was observed for the antibiotic tetracycline [89.5%] and the most sensitivity to antibiotic was reported for chloramphenicol, meropenem and nitrofurantoin [2.9%]. The distribution of dfrA1, sul1, aac[3]-IV, tet[B], tet[A], aadA1, blaCITM, vim, qnr, blaSHV, sim, Oxa-24-like, Oxa-51-like, Oxa-58-like, Oxa-23-like, imp, cmlA and cat1 were 42 [62.6%], 40 [59.7%], 36 [53.7%], 34 [50.7%], 31 [46.2%], 17 [25.3%], 17 [25.3%], 16 [23.8%], 11 [16.4%], 11 [16.4%], 8[11.9%], 5[7.4%], 5[7.4%], 4 [5.9%],4 [5.9%], 4 [5.9%], 2 [2.9%], 1 [1.4%] respectively. Moreover, frequency of class 1, II and III integrons was 67 [100%], 22 [32.8%], and 3 [4.4%] respectively
Conclusions: High prevalence of integrons among Acinetobater baumannii isolated strains in Ahvaz hospitals indicate the importance role of integrons in multidrug resistance in this bacteria. Therefore, unnecessary use of antibiotics are recommended to be avoided
ABSTRACT
Background and Aims: the indiscriminate use of antibiotics can lead to antibiotic resistance in the treatment of infections caused by bacteria such as Pseudomonas aeruginosa. The presence of integrons in Pseudomonas is clearly associated with multidrug resistances. Therefore, this study aimed at tracking class I, II and III integrons of antibiotic-resistant isolates of Pseudomonas aeruginosa that were isolated from nosocomial infection
Materials and Methods: in this study, 51 isolates of Pseudomonas aeruginosa were collected from different wards of Imam Khomeini hospital of Ahvaz since October of 2014 until March of 2015. After identification test and antibigram, coding genes of antibiotic resistance and class I, II and III integrons were detected by polymerase chain reaction [PCR] method
Results: tetracycline revealed the most resistance with 84% frequency in discretedisolates. In the encoding antibiotic resistance genes with a frequency of 94% was the most common blaTEM. Class I integron had 92% prevalence, class II Integron showed 52% prevalence and class III Integron demonstrated 17% prevalence
Conclusions: in Pseudomonas aeruginosa, class I integron was more prevalent than other integrons and the integrase gene was probably one of the causes of multiple antibiotic resistance in this bacteria. Moreover, frequency of integron III was breported 17%
ABSTRACT
Yersinia enterocolitica is a Gram-negative bacterium belonging to the genus Yersinia, family Enterobacteriaceae. In humans, Y. enterocolitica are well-known food-borne pathogens which are mainly transmitted by water and food. Its epidemic often appears after the ingestion of contaminated foods, especially contaminated meat such as chicken. The aim of this study was to determine the presence of Yersinia enterocolitica serotype O:3 in Chicken meat in Shahrekord, Iran. In this study, 300 chicken meat samples were randomly obtained from local supermarkets of Shahrekord. Under strile conditions, 25 g of each sample was taken into 10 ml phosphate buffered saline [pH 7.2] and was homogenized by Stomacher and incubated at 4[degree]C for two weeks. At weekly intervals, a suspension aliquot was cultured linearly on Cefsulodin Irgasan Novobiocin Agar [CIN]. The positive sampals were examined by PCR method, to determine the presence of O:3 serotype. Using culture and PCR method, 65 of the total 300 chicken meat samples were found positive; thus, the prevalence of Y. enterocolitica contamination was 21.66%. Of these, 10 [15.38%] were found to be positive for Yersinia enterocolitica O:3 serotype. The results of this study indicated the presence of Yersinia enterocolitica in chicken meat. Moreover, it was shown that this bacterium is easily transmitted by food and also easily grows in refrigerators at 4 degrees Celsius. Concerning these results, it is recommended that health authorities of Iran consider this issue more seriously, to prevent the health and economic problems which are resulted from it.