ABSTRACT
The role of Interleukin-1 genes in the association between chronic periodontitis and atherosclerosis has been under debate. The purpose of this study is to compare the existence of interleukin 1 genes polymorphism in the chronic periodontitis subjects with and without atherosclerosis. 200 chronic periodontitis patients [184 male, 16 female, mean age 52.61] were divided into two groups: first group 100 subjects with atherosclerosis [92 male, 8 female, mean age 52.06]; second group 100 subjects without atherosclerosis [92 male, 8 female, mean age 53.16]. Probing depth and clinical attachment loss were performed for each patient. Alveolar bone loss was performed for the patients using apical digital radiography. Blood samples were collected from the patients for genotyping analysis of IL-1alpha+4845, IL-1beta+3954, IL-1s-511 and IL-1RN VNTR using PCR-RFLP technique. The Mann-Whitney U and Chai square tests were used for the statistical study [P<0.05] with the computer program SPSS.V17. Clinical attachment loss and alveolar bone loss were significantly higher in the atherosclerosis group. An association between IL-1alpha+4845 genotypes and atherosclerosis was shown; genotype 1.1 was higher significantly in the atherosclerosis and periodontitis group. An association between IL-1alpha +4845, IL1beta+3954, IL-1beta-511 genotypes and alveolar bone loss was also shown. An association between IL-1alpha+4845, IL-1s-511 genotypes and both alveolar bone loss and atherosclerosis was also shown. The IL-1alpha+4845, IL-1beta-511 genotypes may play a role in the association between chronic periodontitis and atherosclerosis in the studied sample
ABSTRACT
Cutaneous Leishmaniasis [CL] is one of the most prevalent clinical forms of leishmaniasis, also it is endemic disease in Syria. And in spite of the great efforts to control the disease the annual incidence is still increased. Furthermore, tourism, migration, and prevalence of acquired immunodeficiency [AIDS] all these factors increase the spread of this disease to new areas around the world. Recently, studies suggest that cytokines gene polymorphisms can contribute to resistance or susceptibility to many diseases and one of these diseases is CL. The main purpose of this research was to study the relationship between the polymorphism in Interferon y [IFN-gamma] gene, which is one of the most important cytokine in the first immune response T helper [Thl] and chronic cutaneous leishmaniasis. In our study we analyzed the polymorphism in Interferon y [IFN- gamma] gene, which is an important cytokine in the first immune response T helper [Thl] .A total of 54 patients with chronic cutaneous leishmaniasis, and 70 healthy controls from different areas in Syria were included in this study. Functional polymorphism in the position +874 of y [IFN-gamma] gene was investigated by polymerase chain reaction and DNA sequencing in cooperation with Tubingen University-Germany. We found that IFN-gamma +874 A ->T polymorphism was less common in patients with chronic cutaneous leishmaniasis CCL compared to the healthy controls [f = 8.81, p = 0.003]. our results suggest that the polymorphism in the first intron of the IFN-gamma gene might influence the progression of disease towards CCL