Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

@#The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the greatest threats to both animal and human health. Our investigation was aimed to identify and differentiate between MRSA and methicillin-sensitive Staphylococcus aureus (MSSA) recovered from mastitic milk using MALDI-TOF mass spectrometry compared with phenotypic methods and studying their susceptibility to various antibiotics. Four hundred milk samples from mastitic animals (cows, sheep, goats, and dromedary camels) were investigated. Phenotypic identification of S. aureus was made through MASTASAPH Latex test, STAPH ID 32, and Vitek 2 system. The proteomic characterization of S. aureus was done by MBT. The Kirby Bauer method was accomplished to detect the resistance of S. aureus strains to antibiotics. The results of the MASTASAPH Latex test, revealed that 54 (46%) were recognized as S. aureus. All S. aureus isolates were identified by MBT with a score of more or equal 2.00. Several peaks were identified in the mass of 4590 Da, 4863 Da, and 4938 Da for MSSA and in the mass of 2636 Da and 3009 Da for MRSA. The MSP dendrogram demonstrated that the S. aureus isolates were classified into one group with a distance level of less or equal 400. The percentage of S. aureus resistance against carbenicillin, erythromycin and kanamycin was 94.4%, 38.88%, and 33.33%, respectively. In conclusion, S. aureus bacteria are among the key triggers for mastitis in Saudi Arabia. MBT is reported to be not only the rapid tool to identify S. aureus but also able to discriminate MRSA from MSSA.

Clinical and laboratory diagnosis of contagious caprine pleuropneumonia in Qassim region, Saudi Arabia: a comparative study

Diagnosis of contagious caprine pleuropneumonia (CCPP) in Saudi Arabia mainlydepends on clinical signs and post-mortem findings, in addition to limited usage of latexagglutination test (LAT). In this study, a PCR method specific for detection of Mycoplasmacapricolum subspecies capripneumoniae (Mccp) was used as a direct confirmatory methodand to compare it with clinical signs, necropsy lesions and LAT. During the 2016-2017 year,samples of serum, pleural fluid, lung tissue and nasal swab were collected from 40 goats withclinical signs of CCPP, which were selected from goats brought to the veterinary clinic ofQassim University from 18 goat herds and nine localities. Epidemiological data revealed34.1%, 27.8% and 81.6% morbidity, mortality and case fatality rates, respectively. At necropsy,31 of 40 goats (77.5%) were found with lesions matching those of CCPP. Molecular findingssupported the suitability and applicability of PCR as a reliable method to diagnose andconfirm CCPP directly from clinical samples. The disease was confirmed by PCR in 35 goatsout of 40 (87.5%), 15 herds out of 18 (83.3%) and in all localities. Sera of 32 goats (80%) werefound positive by LAT. Four of the five goats and two of the three herds negative by PCR werealso negative by LAT and necropsy examination. Therefore, PCR sensitivity was considered97.2% (35/36). Compared to the claimed high specificity and sensitivity of the used PCRmethod, diagnosis of CCPP based on clinical signs was found less specific and necropsyexamination and LAT were less sensitive. It was concluded that molecular detection of Mccpdirectly in clinical samples should routinely be used to confirm diagnosis of CCPP in theregion of study, prevent economic impact of wrong diagnosis and to hasten control process.

A perspective of the epidemiology of malaria and anaemia and their impact on maternal and perinatal outcomes in Sudan

Introduction: Both malaria and anaemia have adverse effects on maternal and perinatal outcomes. Thus there is an urgent need to investigate the co-epidemiology of malaria and anaemia and their combined impact on maternal and perinatal outcomes in the different regions of Sudan.Methodology: Various cross-sectional and case control studies conducted during the years 2003-2010 to investigate the epidemiology of malaria and anaemia and their impact on maternal and perinatal outcomes in different regions of Sudan were compared. Results: While 13.7of antenatal attendants in New Halfa had peripheral microscopically detected Plasmodium falciparum malaria; placental malaria (using histological examinations) was prevalent in 32.0-40and 19.5of parturient women in New Halfa and Gadarif Hospitals; respectively. Malaria was a risk factor for anaemia in New Halfa and for stillbirths in Omdurman Maternity Hospital. Anaemia was present in 52.5; 62.6and 80.2of pregnant women in Medani; New Halfa; and Gadarif Hospitals; respectively. In Gadarif; 57.3of pregnant women had a folate deficiency; while 1had a vitamin B12; deficiency. In Medani; zinc and copper deficiencies were detected in 45.0and 4of pregnant women; respectively. Anaemia was a risk factor for low birth weight in Al-Fashir; for fetal anaemia in New Halfa; and for stillbirth in Kassala Hospital. Conclusion: More care should be taken to ensure proper nutrition and malaria prevention such as bed nets and intermittent preventive treatments to avoid these diseases and their effects on maternal and perinatal outcomes

Morbidity and mortality pattern of neonates admitted into nursery unit in Wad Medani Hospital, Sudan

Neonatal outcome is an important indicator of obstetrics and health care. Few of no published data are available concerning neonatal morbidity and mortality in Sudan. To study morbidity and mortality pattern amongst inborn neonates admitted into nursery unit in Wad Medani Hospital, Sudan during the period Jan-June. 2009. A total of 1211 [29.5%] out of 4098 in-born neonates were admitted during study period. The major indications for neonatal admission were; infections 300 [24.8%, low birth weight [LBW] 307 [25.4%] and asphyxia 130 [10.7]. There were 133 [11%] neonatal deaths; preterm delivery, LBW and birth asphyxia were the major causes of death among these neonates. Neonatal infection, preterm birth and LBW we the common causes of neonatal morbidity and mortality. There is an urgent need for more research throughout the country concerning these common causes of morbidity and mortality

Urological Injuries during Pelvic Surgery in Women; Gezira; Central Sudan

Injuries to the ureter and bladder are common in female pelvic surgery; sometimes extremely common to be considered as traditional. This is a retrospective study conducted in Gezira Hospital for Renal Disease and Surgery; Medani Teaching Hospital and Medani Maternity Hospital; #ad Medani; Sudan. #e included $paetents 'ith urological injuries follo'ing obstetrical gynecology interveneton in the previous ( years. The interaoperaetve injuries consettuted; -.; 'hile the remote injuries 'ere 6+7(.6 -.. #e conclude that the urological injuries are common complications during pelvic surgery; associated mainly 'ith abdominal hysterectomies and Cesarean section

Factors affecting child mortality in Saudi Arabia

To estimate levels and trends, gender differentials, effect of modes of living, regional differentials, and the effect of parental educational on infant and child mortality. A nationwide stratified random sample was used to estimate levels, trends and differentials of infant and child mortality in Saudi Arabia. The study was executed during the period February 2006 to June 2006 and covered all the 20 health regions of Saudi Arabia. The randomly selected sample was limited to ever married Saudi women in the reproductive age group [15-49 years]. The findings show a continuous and considerable infant and child mortality decline during the 1994-2004 period. The infant mortality rate has decline from 22 per 1000 live births in 1994 to 17 per 1000 live births in 2004; while the child mortality rate has declined from 34 to 22 per 1000 live births in the same period. There is a strong relation between infant and child mortality and the mode of living and parental education. The study proves the continuous decline of infant and child mortality during the study period and affirms the effect of parental education on these indicators