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1.
Acta sci., Biol. sci ; 34(3): 311-318, July-Sept. 2012.
Article in Portuguese | LILACS-Express | LILACS, VETINDEX | ID: biblio-1460722

ABSTRACT

Streams Mandacaru, Maringá, Miosótis and Nazareth in the urban area of Maringá, Estado do Paraná, Brazil are affected by the local populations polluting practices. Meristemic cells of Allium cepa L. roots were used as test system to evaluate the cytotoxic effects of the streams water. Onion roots were prepared by Feulgen reaction and stained with Schiff"s reagent. Statistical analysis of the mitotic index was undertaken with the chi-square test. Results did not show significant cytotoxic effect of water samples. Results may dependent on climate variations, concentration of harmful substances, and on the time and samples sites evaluated. A deeper awareness of the population should be underscored for the proper use, preservation and maintenance of soil and water, with particular emphasis to this region.

2.
Acta sci., Biol. sci ; 26(1): 101-105, 2004.
Article in Portuguese | LILACS-Express | LILACS, VETINDEX | ID: biblio-1460274

ABSTRACT

Intense industrial development and population growth have been altering the quality of water and innumerous studies have been undertaken to analyze their effects on humans. Due to rivers contamination with agrotoxics, herbicides, pesticides, excess of farming chemical additives and through sewers spilling not properly treated industrial waste, investigating cytotoxic and mutagenic activity of river water becomes all-important. Bone marrow cells of Wistar rats (Rattus norvegicus), treated in vivo, through gavage, in subchronic treatment (7 days), were used as experimental animal test system to investigate the effects of well water and water from the rivers Ficha and Minas Gerais, close to the municipality of Ubiratã, State of Paraná, southern Brazil. Cell division index and metaphase chromosomes were analyzed. The statistical analysis showed that the experiment"s water failed to alter cell division cycle of Wistar rats and did not increase number of chromosome aberrations. No cytotoxic and clastogenic effects ensued from this treatment


O intenso desenvolvimento industrial e crescimento populacional têm promovido alterações na qualidade da água, sendo esta fonte de muitos estudos para análise desses efeitos no ser humano. Devido a contaminação dos rios por agrotóxicos, herbicidas, pesticidas e demais aditivos químicos utilizados nas lavouras, e pelas indústrias que despejam através de esgotos, resíduos de sua produção, que não são devidamente tratados, torna-se importante a investigação da atividade citotóxica e mutagênica da água dos rios. Neste estudo foi utilizado como sistema teste animal as células da medula óssea de ratos Wistar (,em>Rattus norvegicus), tratados in vivo, via gavagem, de forma subcrônica (7 dias), para investigação dos efeitos das águas do poço, e dos rios Ficha e Minas Gerais, próximos à Ubiratã, Estado do Paraná, Brasil, analisando-se o ciclo de divisão celular e os cromossomos metafásicos. A análise estatística demonstrou que as águas não alteraram o ciclo de divisão celular dos ratos Wistar e também não provocaram aumento no número de aberrações cromossômicas, mostrando não terem neste tratamento, efeito citotóxico e nem clastogênico

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