ABSTRACT
Lucilia sericata larval secretions have bacteriostatic and bactericidal effects. We investigated the effect of L. sericata larval secretions during passage from the third to the second stage on Leishmania tropica under in vitro and in vivo conditions. The L. sericata larvae and their secretions were also used in the treatment of cutaneous leishmaniasis lesions. A total of 29 patients were included in this study. Seven patients were infected with Glucantime-resistant Leishmania major. In 22 patients, there was no information about the Glucantime resistance status and infection with L. tropica or L. major. All patients were efficiently treated with L. sericata larvae and their secretions without leaving scar tissue. Additionally, after 1-2 months of treatment, Leishmania spp. was not detected in the samples using PCR.
ABSTRACT
BACKGROUND/AIMS: It has been reported that proton pump inhibitors induce relaxation in different types of smooth muscles. The aim of this study is to investigate in vitro effects of proton pump inhibitors on human pylorus muscle. METHODS: Pyloric sphincters were studied in 10 patients who were operated for stomach cancer. In isolated organ bath, control and response to rabeprazole were recorded following contraction with carbachol. During the treatment experiment, while distilled water was applied during the control experiment in every 5 minutes, rabeprazole was administered in every 5 minutes at doses of 10-6, 10-5, 10-4, and 10-3 M respectively. Contraction frequencies, maximum contraction values and muscle tones were measured. RESULTS: The contraction frequencies in the control group were greater than the rabeprazole group in the second, third and fourth intervals while the maximum contraction values in the rabeprazole group were lower in the fourth interval. Even though muscles tones were not different in both groups during all intervals, it was remarkable that the muscle tone was significantly decreased in the rabeprazole group during the fourth interval compared to the first and second intervals. CONCLUSIONS: In the present study, high doses of rabeprazole reduced contraction frequencies, maximum contraction values, and muscle tone of human pylorus.
Subject(s)
Humans , Baths , Carbachol , Muscle Tonus , Muscle, Smooth , Muscles , Proton Pump Inhibitors , Pylorus , Rabeprazole , Relaxation , Stomach Neoplasms , WaterABSTRACT
Tumor markers have shown little benefit as a method for screening. However, they can be used clinically for the monitoring of tumor recurrence and used as prognostic factors because higher levels have been observed in advanced disease. This study aimed to investigate the relationship between the preoperative tumor marker levels and different clinical aspects of gastric cancer. One hundred and six consecutive patients with confirmed diagnosis of gastric cancer and 106 subjects [age and sex matched] with no malignancy as control group were included prospectively in this study in 3 years. The relationships between tumor markers CEA, CA 19-9 and stage of disease, tumor differentiation, presence of ringlet cell type, presence of peritoneal carcinomatozis were investigated. The serum CEA and CA 125 levels were found to be significantly elevated in gastric cancer patients than in controls. The serum level of CEA had showed a significant elevation with the presence of distant metastasis. The CA 19-9 and CA 125 levels had showed significant elevations with the presence of peritoneal carcinomatozis. This study showed that there is a limited clinical benefit of preoperative tumor marker measurements in gastric cancer such as estimation of peritoneal dissemination
ABSTRACT
To investigate the effects of enteral and parenteral glutamine [Gln] usage on rats in sepsis. This study was conducted in Istanbul University Experimental Medical Research Institution [DETAE] laboratory, Istanbul University, Istanbul, Turkey between June and September 2009. The levels of blood Gln, tumor necrosis-alpha [TNF-alpha and interleukin [IL]-10 was measured. Samples of tissue were obtained from the mesenteric lymph nodes, liver, and lower lobe of the right lung to evaluate the reproduction of bacteria, and samples of tissue were also obtained from the small intestine to evaluate blood and villus atrophy. Bacteremia of Group EP [combined group] were found lower than Group C [control] [p=0.007]. Villous atrophy rates of all groups were lower than Group C: between Group E [enteral] and C [p=0.003]; between Group P [parenteral alanine] and C [p=0.019]; and between Group EP and C [p=0.001]. The values of serum TNF-alpha and IL-10 of Group EP and P were lower than the other groups at the 24th and 96th hours [p=0.000]. In this study we found that the most efficient Gln administration technique in sepsis was enteral administration together with parenteral administration due to trophic effect on the intestinal mucosa, decrease of reproduction in tissue and blood cultures, immunomodulator effect, and approximately the same cost as parenteral application
ABSTRACT
To investigate the safety of laparoscopic intervention for diagnosis and treatment at 8 mm Hg pressure in one-hour period on acute peritonitis related intra-abdominal sepsis model. In this study, we included 32 female Wistar-Albino rats, weighing 250 +/- 20 g, and divided them into 4 groups. This study was conducted in Istanbul University Experimental Medical Research Institution [DETAE] laboratory from April to May 2009. Intra-abdominal sepsis was created with intraperitoneal [i.p.] one mL [10[9] CFU/mL] Escherichia coli [E. colt] injection, and pneumoperitoneum was formed with CO insufflation at 8 mm Hg pressure for one hour i.p. The rats were administered with: Group 1 - one mL i.p. isotonic saline; Group 2 - one mL i.p. isotonic saline + pneumoperitoneum; Group 3 - i.p E. coli', and Group 4 - i.p. E.coli + pneumoperitoneum. Data were analyzed using the Statistical Package for Social Sciences version 15 for Windows [SPSS Inc, Chicago, IL, USA]. Fever and leukocyte values were considered high in Groups 3 and 4 compared with Groups 1 and 2 [p=0.001]. The administered reproduction ratio of the E. coli strain was determined as 0% in Groups 1 and 2, and 100% in Groups 3 and 4. In this study, as pneumoperitoneum was formed for one hour at 8 mm Hg pressure, in case of intra-abdominal derived sepsis where emergency intervention is needed, we consider that laparoscopic approaches with low pressure may be used safely for diagnosis and treatment