ABSTRACT
Background and Objective: Acute ischemia reperfusion [IR] injury observed in the lower extremities occurs especially when a temporary cross-clamp is applied to the abdominal aorta during aortic surgery. Preoperative pregabalin has been used as a part of multimodal analgesia in postoperative pain treatment in recent years. Pregabalin has become one of the increasingly common agents in postoperative analgesia. In this study, we aimed to investigate the effect of pregabalin on erythrocyte deformability in rats undergoing IR
Methodology: 24 male Wistar albino rats weighing between 200-250 g were used in the study. Rats were randomly divided into 4 groups of 6 rats each [Control, Ischemia- Reperfusion [IR], IR-Pregabalin 50 mg [50 mg/kg], IR-Pregabalin 200 mg [200 mg/ kg]. Pregabalin was administered intraperitoneally 30 min before the procedure. An atraumatic microvascular clamp was placed across the infrarenal abdominal aorta in the IR groups. Following 120 min of ischemia, the clamp was removed and reperfusion was continued for 120 min. All rats were euthanized by intraperitoneal administration of ketamine [100 mg/kg] and taking blood from the abdominal aorta. Erythrocytes were seperated from heparinized whole blood samples. Deformability measurements were made in erythrocyte suspensions in phosphate buffered saline. A constant flow filtrometer system was used to measure erythrocyte deformability and relative resistance was calculated
Results: It was found that the formation of ischemia reperfusion increases the relative resistance according to the control group [p < 0.0001]. It was determined that application of pregabalin 50 or 200 mg did not change erythrocyte deformability in ischemia reperfusion-induced rats [p = 0.632, p = 0.811]
Conclusion: The administration of 50 or 200 mg of pregabalin has no negative effect on the erythrocyte deformability in ischemia reperfusion-induced rats. We think that pregabalin can be safely used for analgesia in the cases of IR. However, these findings should be supported by clinical and experimental studies carried out in more detailed and broader series
ABSTRACT
To investigate the effects of lidocaine on the morphology of saphenous veins [SVs] harvested during coronary artery bypass graft [CABG] surgery. This experimental study was conducted at the Cardiovascular Surgery Department, Gazi University, Ankara, Turkey, between May and September 2007. The SVs from 11 patients who underwent CABG surgery were divided into 3 segments. Each segment from the same location of the grafts was allocated into 3 groups as control group [group C], physiologic saline group [group PS], and lidocaine group [group L]. Nitric oxide synthase [NOS], nitric oxide [NO] pool, super oxide dismutase [SOD], and thiobarbituric acid reactive substances [TBARS] levels were measured in the samples from the groups. Histologic specimens were evaluated according to previously defined criteria, and scored accordingly. Histological examination of the grafts in groups L and C were similar, but histological scoring of grafts in group PS were statistically higher than group C [p=0.008]. Nitric oxide synthase activity and NO pool were higher in groups L and PS than in group C [p=0.010]. Super oxide dismutase activity was higher in group L than in group PS [p=0.008]. Super oxide dismutase activity was lower in group PS than in group C [p=0.047]. There was no significant difference between TBARS level in all groups. Our results indicate that primary damage might occur during surgery due to traumatic handling of the graft, and succeeding injuries could occur due to ischemia-reperfusion injury during the waiting period. Adding lidocaine to the preservation solution will protect later injury