ABSTRACT
Aim: The aim of this study was to design multi-walled carbon nanotubes [MWCNTs] loaded with paclitaxel [PTX] anti-cancer drug and investigate its anti-cancerous efficacy of human gastric cancer
Background: Carbon nanotubes [CNTs] represent a novel nano-materials applied in various fields such as drug delivery due to their unique chemical properties and high drug loading
Patients and Methods: In this study, multi-walled carbon nanotubes [MWCNTs] pre-functionalized covalently with a paclitaxel [PTX] as an anti-cancer drug and evaluated by different analyses including, scanning electron microscope [SEM], particle size analyzer and cellular analyses
Results: A well conjugated of anti-cancer drug on the carbon nanotube surfaces was shown. This study demonstrates that the MWCN-PTX complex is a potentially useful system for delivery of anti-cancer drugs. The flow cytometry, CFU and MTT assay results have disclosed that MWCNT/PTXs might promote apoptosis in MKN-45 gastric adenocarcinoma cell line
Conclusion: According to results, our simple method can be designed a candidate material for chemotherapy. It has presented a few bio-related applications including, their successful use as a nano-carriers for drug transport
ABSTRACT
Poly vinyl alcohol/Chitosan nanofibrous mat were prepared by electrospinning method with suitable pore sizes as potential matrices for soft tissue engineering. The designed scaffolds by electrospinning method evaluated by differentanalyses such morphological, mechanical, and cellular analysis.Microscopic results showed diameters of poly vinyl alcohol/Chitosan nanofibers were approximately 150 nm. Mechanical investigations illustratedstress - strain curve of poly vinyl alcohol /chitosan mat indicate good flexibility with average strain and good percentage of yield stress. The cellular resultsrevealthat addition of chitosan to poly vinyl alcohol enhances viability and proliferationof fibroblast cells, which increases the biocompatibility of the scaffold. In fact, addition of a smallpercentage of chitosan to the poly vinyl alcoholproved to be a promising approach for designof a scaffold
ABSTRACT
Background: Various regions in Iran, especially the Khuzestan Province, have been covered by dust and dirt during the past two years due to environmental changes in the Middle East. We sought to evaluate the effect of these pollutants on the coagulant factors of people residing in Abadan and Khoramshahr, two major cities of Khuzestan Province
Methods: One hundred twenty-nine healthy individuals were enrolled into this study, and their prothrombin time as well as fibrinogen, platelet, and Factor VIII levels were measured before and after climate changes
Results: After climate changes, the mean prothrombin time decreased, while the fibrinogen, platelet, and Factor VIII levels rose
Conclusion: The results of this study suggest that the pollutants deployed in the Middle East can affect prothrombin time as well as fibrinogen, platelet, and Factor VII levels considerably and increase coagulant state. The pollutants can, consequently, increase the risk of cardiovascular diseases. It seems that cooperation at government levels between Iran and its neighboring countries is required to reverse desertification and avoid inaccurate usage of subterranean water resources so as to lessen air pollution
ABSTRACT
The antidepressant effects of aloe vera hydro alcoholic extract at different concentrations were compared with the fluoxetine-treated and the control groups of mice using forced-swimming, FST and open box, OFT tests. The mice were evaluated in five groups [control, taking aloe vera at the dosage levels of 150 mg/kg, 300 mg/kg, and 450 mg/kg, and finally fluoxetine at a dose of 10 mg/kg] by the FST and OFT tests on 1[st], 7[th], and 14[th] days. The results of the OFT test showed no significant differences between these five groups. The results of FST test indicate the antidepressant effects of aloe vera even at low doses and it was found that the effect of fluoxetine at a dose of 10 mg/kg was equivalent to the effect of aloe vera at a dose of 150 mg/kg for the reduction in immobility time in mice in FST test. According to the results obtained from FST test, the antidepressant effects on mice treated with the 450 mg/kg dose of aloe vera showed better recovery as compared with other groups on 1[st], 7[th], and 14[th] days. With regard to the experiments performed at different times, all the evidence pointed to the conclusion that the antidepressant effect of aloe vera was more than the control group. Based on the results of the OFT and FST tests, aloe vera extract at different doses, has favorable antidepressant effects on mice as compared to the fluoxetine-treated and the control groups and the better effects were seen by increasing the dose and duration of drug use
ABSTRACT
Thermo-sensitive hydrogels were prepared by graft copolymerization of chitosan and N-Isopropylacrylamide via gamma radiation. Characterization of hydrogels such as 13C-NMR, DSC analysis and swelling test and cell assessments for harvesting living cell sheet were investigated. 13C-NMR and DSC analysis showed chitosan and NIPAAm monomer were grafted via gamma radiation successfully. Swelling ratio and curves results administrated hydrophilicity / hydrophobicity of hydrogel that this property is due to presence of PNIPAAm in different temperatures. The hydrogel was tested for harvesting epithelial cells after carrying out cell culture at 37 degreeC and incubating the confluent cells at 4 degreeC for spontaneous detachment of cell sheet from hydrogel surface without enzyme treatment. Cell viability assay results and microscopic observations demonstrated that cells could attach to the hydrogel surface and maintain high viability and proliferation ability. Cell detachment efficiency from the hydrogel was high. These unique properties of the hydrogel would make it a promising support for epithelial cell grafting especially cornea regeneration
ABSTRACT
Cellular orientation control is important for tissue regeneration. Design of oriented structures for cells with suitable features can be used in tissue engineering. One of the methods of cellular orientation with the aim of regenerating which damaged tissues is utilizing oriented biocompatible substrates. Different methods can be used to design these structures such as utilizing magnetic fields, electrospinned oriented fibers or methods such as directional solidification. This paper investigates the influence of grooved substrate-mediated physical guidance on the growth and alignment of cells in vitro. A novel technique was developed to fabricate microgrooves on biodegradable polymer substrates made of poly hydroxyl buthyratevalerate. Solvent-castings were used to transfer micro patterns from quartz and silicon substrates onto biodegradable polymer films. The cells were successfully oriented on micro grooved polymeric substrate which can be used for axon guidance and nerve regeneration