ABSTRACT
Background: Sirtuin-1 [SIRT-1], a protein has been found to protect the cells against oxidative stress due to its deacetylase activity. In this investigation, we aimed to study SIRT-1 gene rs2273773 C >T single nucleotide polymorphism and markers of serum protein oxidation [protein carbonyl and sulfhydryl groups] in asthmatic patients
Subjects and methods: 120 asthmatic patients and 120 healthy controls were genotyped for SIRT- 1 gene rs2273773 C > T SNP using polymerase chain reaction - confronting two pair primer method [PCR-CTPP]. Serum protein carbonyl and sulfhydryl groups were measured using colorimetric methods
Results: SIRT-1 gene rs2273773 C> T SNP genotyping revealed that the TT genotype was significantly higher in the patients compared to the controls [P < 0.05], while there were no significant differences regarding the genotypes TC and CC between the patients and the controls [P> 0.05]. T allele was significantly higher in the patients compared to the controls [P =0.017]. The distribution of the genotypes didn't differ among the atopic and the non-atopic asthmatic patients, also no difference was found in the genotype distribution according to the severity of asthma [P >0.05]. Serum protein carbonyl group concentration was significantly higher in the patients compared to the controls [P < 0. 001], while serum protein sulfhydryl group content decreased significantly in the patients compared to the controls [P < 0.0001]. No differences in markers of protein oxidation according to SIRT-1 gene rs2273773 C > T genotype were found
Conclusion:SIRT-1 gene rs2273773 C> T SNP was associated with asthma but not with protein oxidation markers in Egyptian population