ABSTRACT
Introduction: Atherosclerosis is most common cause of acute myocardial infarction [MI] and can lead to increased oxidative stress and damage to macromolecules. The aim of this study was to determine the amount of 8-Hydroxydeoxyguanosine [8- OHdG] as a marker of damage to the DNA and the levels of heat shock protein HSP27 in acute myocardial infarction [MI] patients
Materials and Methods: In this case-control study, 30 patients with acute MI were enrolled. Serum levels of HSP27, 8-OHdG, cardiac troponin I [C-TnI], creatine kinase [CK-MB] and levels of total antioxidant and malondialdehyde were assessed. Data were analyzed by SPSS 16 and T-test analysis at the significant level of P50.05
Results: Data analysis showed that serum levels of HSP27 and 8-OHdG in acute MI patients peaked [8-OHdG=9.8 +/- 2.1, HSP27=81 +/- 3.1] after 48 hours of the attack showing a significant increase [HSP27=9.7 +/- 1.8, 8-OHdG=4.4 +/- 1.2] [P50.05] in comparison to healthy controls. Levels of the enzyme CK-MB 24 hours and CTnI and malondialdehyde after 48 hours after acute MI showed the highest values
Conclusion: Increase in markers of DNA damage and heat shock protein levels in MI patients, verify high levels of oxidative stress in MI, indicating that evaluation of changes in 8-OHdG and heat shock proteins is a valuable way to assess the level of damage to macromolecules during acute MI