[Association between amino acid sequences in peptide binding region [BOLA-DRB3] and susceptibility or resistance to calf diarrhea]

The major histocompatibility complex genes [mhc] encode MHC I and II molecules which present peptide fragments to T cells. Therefore these polymorphic molecules critically influence susceptibility to infectious diseases. At present study potential relationship between amino acid sequences in the antigen binding groove of different BoLA-DRB3 alleles and susceptibility or resistance to calf diarrhea was investigated. Twelve different DRB3 alleles were found among 171 calves [84 diarrheic and 87 healthy] analyesd by PCR-RFLP method. Amino acid sequences of the encoded peptide binding region were compared. 26 polymorphic positions were detected in this region. A significant association [p<0.05] was shown between occurrence of diarrhea and the presence of glutamic acid and tyrosine inpocket 4 and valine, glutamine and leucine in pocket 9 of peptide binding region. Thus it can be concluded that pockets 4 and 9 of the BoLA-DRB3 molecule would be involved in conferring susceptibility of calf to diarrhea

Biosorption of nickel [II] from aqueous solutions by marine red alga gracilaria corticat

The kinds of pollutants such as heavy metals, especially, can be removed from water and waste water by biological materials via biosorption.These heavy metals can be also recovered by adsorbents. The mentioned methods have more importance due to the advantages such as cheapness. In this study marine red alga Gerasilaria sp. was used as an aquatic fern to remove Ni2+in the flow operation. A glass column with an internal diameter of 2 Cm and 35 Cm in length were investigated as a packed column. The adsorption process with influencing factors such as initial concentrations, pH, retention time [15, 30 and 45 min], and adsorbent dosage [2.5 and 3.5 g] in temperature home were considered. Synthetic solution of Ni [II] in distilled water was prepared using NiCl[2].6H[2]O [97%] with initial concentrations 25, 40, 70 mg/L. The maximum uptake at pH=5 were obtained 83.01%, 80.69% and78.23% respectively. An atomic absorption spectrometer [GBC-932] was used for analyses of the artificial waste after exposure to alga. It was shown that the non-living Gerasilaria sp. adsorb according to Langmuier physical low and the kinetic studies was appeared that all of the used non-living alga samples adsorbed according to a second order model of kinetic