ABSTRACT
The objective of this study was to evaluate the effects of myo-inositol alone or in combination with MEM vitamins on embryo development in sanjabi sheep. Sheep Cumulus Oocytes Complexes [COCs] were matured in vitro at 39 °C, in humidified 5% CO2 atmosphere for 22-24 h. There were three treatments, culture in synthetic oviductal fluid medium [treatment I], culture in synthetic oviductal fluid medium supplemented with1 x MEM vitamins [treatment II], culture in synthetic oviductal fluid medium supplemented with myo-inositol [treatment III], COCs were then fertilized and cultured in vitro for and days when the ratios of in vitro embryo development of the hatched blastocysts were assessed and compared with the control group. The presence of myo-inpsitol significantly improved overall morula rates [34.39%] than that of control group [24.18%], but there was no difference between myo-inositol and 1 x MEM vitamins in the percentage of embryos successfully developing to the morula stage [p<0.05]. The addition of myo-inositol improved the mean blastocyst formation compared to the oocytes matured in medium containing no MEM [control] or 1 x MEM vitamins [32.63, 13.96 and 21.06% respectively]. However, the mean percentage of cleavage rate was not substantialy different between treatments. These results suggest that adding myo-inositol to SOF medium be more beneficial for subsequent sheep embryonic development
Subject(s)
Animals, Laboratory , Inositol/pharmacology , Vitamins , Sheep , Fertilization in VitroABSTRACT
Lysozyme is an antimicrobial protein widely distributed among eukaryotes and prokaryotes and take part in protecting microbial infection. Here, we amplified cDNA of MesoLys-C, a c-type lysozyme from the most common scorpion in Khuzestan Province, Southern Iran. Scorpions of Mesobuthus eupeus were collected from the Khuzestan Province. Using RNXTM solution, the total RNA was extracted from the twenty separated venom glands. cDNA was synthesized with extracted total RNA as template and modified oligo [dT] as primer. In order to amplify cDNA encoding a lysozyme C, semi-nested RT-PCR was done with the specific primers. Follow amplification, the fragment was sequenced. Sequence determination of amplified fragment revealed that MesoLys-C cDNA had 438 bp, encoding for 144 aa residues peptide with molecular weight of 16.702 kDa and theoretical pI of 7.54. A putative 22-amino-acids signal peptide was identified. MesoLys-C protein was composed of one domain belonged to c-type lysosyme/alphalactalbumin. Multiple alignment of MesoLys-C protein with the related cDNA sequences from various organisms by ClustalW program revealed that some of the conserved residues of other c-type lysosymes were also seen in MesoLys-C. However, the comparison suggested that Mesobuthus eupeus of Khuzestan and east Mediterranean Mesobuthus eupeus belonged to different subspecies