[Prevalence of hepatitis B in beta -thalassemic patients in Ardabil during 1389]

Hepatitis B virus [HBV] is one of the most important health problems transmissible through blood transfusions. Generally, a number of people with ?-thalassemia are susceptible to HBV since they receive multiple blood transfusions. These people have high infection rates despite vaccination against HBV. In this study, we explore the seroprevalence and risk factors of HBV infection in thalassemic patients who reside in Ardabil. This was a descriptive study on 120 thalassemic major patients who referred to Bo-Ali-Sina Hospital in Ardabil. Patients' demographic characteristics and blood samples were obtained The ELISA technique detected the presence of HBsAg, HBsAb and HBcAb. We used SPSS software and the chi-square test for statistical analyses. Of 120 patients, there were 64 males and 56 females. The median age was 17.3 +/- 4.5 years. Prevalence of HBsAg was 3.3%. There were 29.2% of patients HBsAb positive and 4.16% HBcAb positive. Statistical analysis showed no significant correlation between transfusion times and serological markers [HBsAg, HBsAb, HBcAb]. Of note, there was a meaningful relationship between duration of disease and HBsAb markers [p < 0.05]. The prevalence of HBV in thalassemic patients in Ardabil was 3.3%. According to the results,show that infection rates could be mediocre than to population of this province. High quality transmission of blood products effectively decreases incidence of infection

[Effect of endogenous nitric oxide on cardiac ischemic preconditioning in rat]

Ischemic Preconditioning [IPC] is the phenomen that happens on the heart by one or several short periods of ischemia followed by reperfusion that improve the postischemic recovery of mechanical function. Ischemic preconditioning [IPC] may protect the heart from ischemia reperfusion injury by nitric oxide formation. This study investigated the effect of ischemic preconditioning on heart and the relationship between nitric oxide. 28 male Sprague dawley rats [200-250 g] in Tehran University of Medical Sciences were used. Rats were anesthetized and hearts were rapidly isolated and perfused in the Langendorff mode at a constant perfusion pressure and temperature of 37°C. hearts were divided to 4 groups. Control group was perfused 170 minutes with buffer solution. ischemic reperfusion [IR] group was subjected to 30 minutes ischemia. [Ischemic preconditioning] IPC group was elicited by 5 min ischemia followed 5 min reperfusion before IR and L-NAME + IPC group, L-NAME [0.1mM] was added into the perfusion solution. Heart rate [HR], left ventricular development [LVDP], RPP [LVDPXHR], infarct size and coronary flow [CF] were measured. ANOVA tests[with TUKEY post test if p<0.05] were used for statistical analyses. IPC improve the postischemic recovery and reduced postischemic ventricular dysfunction in heart and reduction of infarction size. No significant differences were observed between IPC and IPC + L-NAME groups. L-NAME did not affect postischemic recovery of IPC so in the isolated heart NO isn't involved in the cardioprotective effect of IPC

role of K-ATP channel in the preconditioning effect of magnesium in the rat isolated heart

There is growing interest for beneficial effect of Mg in the cardiovascular disorders. A number of cardiovascular disorders including myocardial infarction, arrhythmias and congestive heart failure have been associated with low extracellular or intracellular concentrations of Mg. The aim of present study was to investigate the preconditioning effects of magnesium [Mg] on cardiac function and infarct size in the globally ischemic-reperfusion in isolated rat heart. Rat hearts were Langendorff-perfused, subjected to 30 minutes of global ischemia and 90 minutes of reperfusion, and assigned to one of the following treatment groups with 7 hearts in each group: [1] control, [2] ischemic- reperfusion, [IR], [3] ischemic preconditioning, [IPC] of 5 minutes of global ischemia - reperfusion before lethal ischemia; or pretreatment with [4] 30 Mu mol/L of Diazoxide [Dia], [5] 8 mmol/L magnesium, [6] 10 Mu mol/L glibenclamid [Gli], [7] magnesium and Dia and [8] magnesium and Gli. Infarct size was measured by the triphenyltetrazolium chloride method. Left ventricular function was assessed by left ventricular developed pressure [LVDP], heart rate and coronary flow [CF]. Mg limited infarct size [9.76% vs 44.47% in IR, P< 0.001] as did Dia [10.2% vs 44.4% in IR P< 0.001] and IPC [8.69% vs 44.47% in IR, P< 0.001]. The protective effect of magnesium was abolished by Gli. Administration of Mg had an anti-infarct effect in ischemic-reperfusion isolated rat hearts and improved cardiac function. Blockade of K-ATP channel abolished the protective effects of magnesium and suggest that K-ATP channel has an important role in this effects