Effect of betadine on wound healing in rat

Betadine is a disinfectant routinely used in all of the Iranian health centers and many other countries in the world but its effect on wound healing is controversial. The aim of this study was to investigate the effect of betadine on wound healing in rat. In this interventional study, two groups of rats [n=10 each] were randomized as betadine and control groups. Under nesdonal anesthesia, a full thickness skin in an area of 2 cm[2] was excised on dorsal neck of rats and immediately betadine was applied in betadine group. Control group received no treatment. Wound area percentage of recovery and body weight were measured on postoperative days 1, 4, 7 and 10. Number of fibroblasts, collagen fibers, new vessels, lymphocytes, macrophages, neutrophils and thickness of epiderm were determined in three groups by biopsy on day 10. The results showed the percentage of recovery was not significantly different in the 4th, 7th and 10th days. Revascularisation, fibroblasts, lymphocytes, neutrophils, epidermal depth and collagen fibers were not significantly different between two groups. Macrophages in the betadine group were significantly higher than control group. The results propose that betadine not only does not speed up wound healing but also delays the inflammatory phase

[Effects of morphine dependency on the ovarian folliculogenesis following superovulation in mice]

Opioids may affect hypothalamic GnRH secretion and Hypothalamic-Pituitary- Gonad axis, resulting in reproductive disturbances. Current study investigates the effects of morphine on structure of ovary following superovulation through morphologic/morphometric studies. Twelve young female NMRI mice were allocated into treatment and control groups. Treatment group received oral morphine at final dose of 0.4mg/ml for 21 days. Physical dependency was proved by injection of naloxone [2mg/kg ip]. The mice were superovulated by 10 iu PMSG [ip] and 48 hours later were sacrificed by cervical dislocation. Ovaries were removed and H and E staining was done. Every 10[th] serial section, which represents nonrandom 10 percent sample was counted. Follicles were classified into small, growing, antral and atretic according to the diameter and number of follicular cell layers surrounding oocytes. The volume and the weight of ovaries were recorded. In addition, the diameter of the antral follicles and oocytes was carefully measured by a calibrated oculometer. The volume and the weight of ovaries showed no significant alterations in the two groups. The proportion of small and atretic follicles was statistically different in treatment and control groups [P<0.001]. According to our data, oral morphine did not alter the volume and the weight of the ovary. However, folliculogenesis was moderately affected by morphine and following superovulation the behavior of ovaries in the treatment group is comparable to the control group

[Comparison of the effect of infrared [IR] and Phenytoin cream on skin wound healing in rat]

Background and Objective: Infrared [IR] is one of the modalities in electrotherapy that indicates for treatment of some diseases with minimal side effects, but there is different ideas about its effects on wound healing. The aim of this study was to investigate effects of IR on skin wound healing in rat and its comparison with phenytoin's effect

Methods: This interventional and experimental study was done on three groups of rats. After inducing general anesthesia in rats, skin wound was made on dorsal neck about 2 cm[2]. Then from third day after operation, IR and phenytoin cream [1%] were indicated in two groups. No treatment was administered for control group. Wound surface area, percentage of recovery and rat weight change post operation at 1[st], 4[th], 7[th] and 10[th] day were measured. Number of fibroblasts, collagen fibers, new vessels, lymphocytes, macrophages, neutrophils and thickness of epiderm were determined in three groups by biopsy at 10[th] day

Findings: The results showed that percentage of recovery was not significantly different in three groups at 4[th] day. But, percentage of recovery at 7[th] day in IR, control and phenytoin group were 51.5+/-17.7, 38.8+/-17.9 and 55.3+/-10.1, respectively [P<0.04] and at 10[th] day in IR, control and phenytoin group were 68.4+/-17.5, 57+/-18.4 and 78.9+/-8.2, respectively [P<0.01]. Number of new vessels, fibroblasts, macrophages, lymphocytes, neutrophils and thickness of epiderm were not significantly different in three groups after 10[th] days. Number of collagen fibers was significantly different in three groups [P<0.01]

Conclusion: Findings suggest that infrared accelerates wound healing from 7[th] day that this effect is the same as phenytoin's healing effect