[Glycodelin expression in human breast cancer]

Glycodelin expression in normal and cancerous human breast tissue and its relation with age, tumor type, microscopic grade and metastasis to axillary lymph nodes recently were noticed. This study was done to evaluate the glycodelin expression in breast cancer. In this descriptive study, 96 Paraffin-embedded blocks of malignant breast cancer by immunohistochemistry method were considered to evaluate the expression of glycodelin. Patients age,tumor size, tumor type, microscopic grade and metastasis to axillary lymph nodes were recorded for each subject. Glycodelin was found in 30.45% of invasive carcinoma of the breast with axillary lymph node metastasis. Glycodelin was expressed in 72.7% of carcinoma of the breast without lymph nodes metastasis [P<0.05]. There was a significant relation between glycodelin expression with microscopic grade 1 of tumor and metastasis to less than 4 of lymph nodes [P<0.05]. Glycodelin expression can be evidence for lack of axillary lymph node metastasis of breast cancer

[Peritoneal benign cystic mesothelioma in a patient with presentation of ascites, a case reprot]

Cystic mesotheloma is an uncommon lesion of the peritoneum occurring predominantly in women of reproductive age. The case was a 21 years girl presented with 4 years history of mild abdominal distension and periodic pain. The results of the clinical examinations were normal. Sonography and CT scan confirmed gross ascites. The results of paraclinical tests were normal without any positive findings for etiology of acites. During laparoscopy multiple transparent cysts were found in pelvic and culdesac. All cysts were removed by laparoscopy. Histology confirmed benign cystic mesotheloma. Reviewing the records revealed that this case is the second case of mesothelial cysts that presented with ascites

[Comparison of the adequacy of specimens provided by fine needle aspiration, fine needle non - aspiration sampling and combined technique in thyroid nodules]

Thyroid dysfunctions are one of the most important diseases in endocrinology. Fine needle aspiration [FNA] is currently the first line diagnostic tool for the evaluation of thyroid nodules. The adequacy of specimens provided by this method is also very important. FNA puncture in thyroid causes intra- parenchyma hemorrhage, which may distribute the quality of tissue specimens. Fine needle non-aspiration [FNNA], may provide better specimens without this problem. The aim of this study was to evaluate the adequacy of thyroid nodules specimens provided by fine needle aspiration and fine needle non - aspiration sampling in Imam Hospital. This descriptive study was performed on 200 patients with thyroid nodules in Imam Hospital, Sari, Iran. After a detailed clinical history, systemic examination and thyroid sonography, FNA and FNNA sampling were performed in all patients. The adequacy of specimens was evaluated by Mair scoring system in both techniques. All data, including demographic data and sonographic results and background blood, amount of cellular material, degree of cellular degeneration, degree of cellular trauma and retention of appropriate architecture were recorded. Results were analyzed by means of SPSS [11] software and Wilcoxon Signed Rank Test. The P.value less than 0.05 were considered to be significant statistically. 200 patients, 189 [94.5%] females and 11 [5.5%] males, with mean age of 43.36 +/- 12.3 years were studied. Cytological findings showed that in FNA technique, 26[13%] of specimens were unsuitable for diagnosis, 97[48.5%] were diagnostic/adequate, while 77[38.5%] were diagnostic/superior for diagnosis. In FNNA technique, 32[16%] of specimens were unsuitable, 96 [48%] were diagnostic/ adequate, while 72 [36%] were diagnostic/superior for diagnosis. In the combined application of FNA and FNNA, 9 [4.5%] of specimens were not diagnostible and 191 [95.5%] were diagnostic. Mean of total score in FNA was 5.82 +/- 2.3, while in FNNA was 5.70 +/- 2.4, with the difference was not statistically significant. Furthermore, there was no statistically significant difference between other parameters. Our study demonstrates that there is no difference between FNA and FNNA sampling, however, applying the combination of these two techniques, may improve the diagnostic quality

[Relationship between cervicovaginal ferritin levels in 22-26 weeks of pregnant women with spontaneous preterm delivery]

Low serum ferritin levels correlate with low iron stores, whereas high levels are associated with an acute-phase reaction. Our objective was to determine whether elevated levels of ferritin in the genital tract can be a potential marker to identify patients at risk for spontaneous preterm delivery. We performed a nested case-control study involving 28 women who had spontaneous preterm delivery. 28 term control subjects mathched for parity, age, education, previous history of PTL and vaginal bleeding selected from 275 women enrolled in the preterm prediction study of the prenatal care unit at Imam Hospital. Cervical ferritin was measured using radoimmunoassay. Cervical ferritin levels were significantly higher in women who subsequently had spontaneous early preterm delivery [mean +/- SD: 54.5 +/- 5 ng/ml VS 36.39 +/- 1.59 in control, P=0.001]. Elevated cervical ferritin levels in 22=24 weeks of gestation in asymptomatic women are associated with subsequent spontaneous preterm birth. Cervical ferritin, alone or in combination with other potential markers, maybe a useful tool to help identify high risk women who would potentially benefit from such prospective intervention. Until the identification of such clinical interventions in a clinical trial, the use of cervical ferritin for the prediction of preterm delivery remains experimental

Diagnosis validity of AgNOR staining for differentiating non Hodgkin lymphoma from reactive hyperplasia

There are different methods including H and E, imonhistochemstry, flu cytometry, AgNOR staining for differentiation reactive hyperplasia from lymphoma. This study was done to determine diagnostic validity of AgNOR staining for differentiating non-Hodgkin lymphoma from reactive hyperpalsia. Fifty paraffin blocks belong to patients with confirmed diagnosis are as below: Thirty five block with lymphoma and fifteen blocks with reactive hyperplasia. AgNor dots among one hundred cells were counted, by two experienced pathologists. The mean of AgNOR dots were calculated. Student T- test used to compare mean AgNOR dots. The mean AgNOR count was 2.2 [range 1.4- 3, SD=0.8] in reactive hyperplasia and 6.7 [range 3.9- 9.5, SD=2.8] in non-Hodgkin lymphoma. A statistically significant difference was observed between the AgNOR count of reactive hypeerplasia and non-Hodgkin lymphoma [p<0.05]. The findings support the validity of AgNOR technique for differentiating and confirming non-Hodgkin lymphoma from reactive hyperplasia