[Effect of cow's milk elimination from children diet on chronic constipation]

Children constipation is one of the common causes of abdominal pain. Cow's milk in children diet is suggested to be one of the main cause of chronic constipation. This study was performed to determine the effect of cow's milk elimination from children diet with chronic constipation. This quasi-experimental study, was performed on 80 children with chronic constipation. Rome-III criteria were considered for disease diagnosis. Cow's milk was eliminated for three weeks from children's diet and the clinical symptoms in children were surveyed, subsequently. Constipation was cured in 33.8% of children as result of cow's milk free diet. The rate of treatment response in affecting children whom their disease in began under 2 years of age [52.6%] was significantly higher than others [16.6%] [P<0.05]. Children treatment was not significantly related to child's gender and amount of milk consumption. Eliminating of cow's milk from the diet improves the chronic constipation in children

Evaluation of Juglans regia pericarp on antifungal susceptibility with broth dilution method

Antifungal property of Juglans regia has not been proved yet, therefore, the present study we designed to examine the effect of Juglans regia pericarp on the growth of different fungi, particularly dermatophytes. The extract has been prepared by methanol solution using percolation method according to NCCLS protocol. Then, the fractions have been examined on fungi inoculums. For MBC estimation, it has been cultured on PDA medium. Juglans regia extract completely prevented growth of Epidermophyton floccosum and Trichophyton mentagrophytes in fraction of 120 mg/ml, Microsporum canis in fraction of 450 mg/ml, and Candida albicans in fraction of 337 mg/ml, meanwhile, it reduced colony growth of Aspergillus niger compared with the control. We have demonstrated that the growth of three dermatophyes and Candida albicans was completely inhibited by Juglans regia pericarp extract

[ vitro evaluation of the susceptibility of dermatophytic and saprophytic fungi to Pistacia vera's pericarp extract]

The purpose of this study was to evaluate the effects of Pistacia vera's pericarp extract on some common dermatophytic and saprophytic fungi of Iran and identifying its probable role to be used instead of chemical drugs. After collecting pericarp of Pistacia vera, drying and making it powder, extracts were obtained by using Percolation method with methanol and n-Hexan. To evaluate the anti-fungal activity of the extract, different dilutions of the extract [30-600 mg/ml] were prepared and tested against each fungus and Minimum Inhibition Concentration [MIC] was measured via disc diffusion and broth dilution methods. The tested fungi were three types of dermatophytes [Trichophyton mentagrophytes, Microsporum canis, Epidermophyton floccosum] and two types of saprophytes [Aspergillus niger and Candida albicans]. The results showed that n-Hexan extract in disc diffusion method has no significant effect on the fungi, but it could inhibit Epidermophyton floccosum growth in 337mg/ml dilution and Microsporum canis growth in 450mg/ml dilution. For methanolic extract in broth dilution method, 60mg/ml was inhibitor for Epidermophyton floccosum and Trichophyton mentagrophytes and 240mg/ml dilution for Microsporum canis growth. In disc diffusion method we had 17 millimeter inhibitory zone around the pure extract in Trichophyton mentagrophytes. This extract had no anti-fungal effect against Aspergillus niger, but inhibited the growth of Candida albicans in 120mg/ml dilution and also calculated MBC for Candida albicans was 600mg/ml. Our research showed Pistacia vera's pericarp extract has different anti-fungal effects on experimented fungi