ABSTRACT
Dot-ELISA assay is a solid phase diagnostic method for detection of antigen or antibody that is used widely for diagnosis of protozoan and metazoan diseases of human and animals. To evaluate this method in diagnosis of human toxoplasmosis, the test was standardized, using golden positive and negative serum samples. Then 215 human serum samples were evaluated for IgG and IgM against Toxoplasma gondii by Indirect Immuno-Fluorescent Antibody [IFA] and Dot-ELISA. Using statistic program, Epi info 6.0 showed these testes have a good agreement in diagnosis of toxoplasmosis with Kappa = 0.8607 for lgG and Kappa = 0.8865 for IgM antibodies [P<0.05]. This study and the works carried out by the other scientists indicate that the Dot-ELISA test is rapid, simple, cost effective, does not need expensive equipment and has a good sensitivity and specificity. But, as the results are expressed qualitatively, therefore, it is not possible to use for antibody titer determination. Yet, it is quite useful for screening test especially in the field and where there are no well-equipped laboratories
ABSTRACT
To evaluate the response of delayed hypersensitivity in the sheep immunized against theileriosis, 80 sheep in 3 experimental groups and 3 control groups were inoculated [i. d.], in the lateral neck area with normal and 1/10 normal theilerine. The response to the theilerine, as appearance of nodule and thickening of skin at the site of inoculation, was observed and measured after 72 hours. From the vaccinated sheep, 71.42% and the recovered sheep, 80.0% showed positive reaction against theilerine, revealing 1 mm or more of thickening skin. This research resulted in understanding that firstly, dermal test can be useful for evaluation of immunity in the vaccinated animals. Secondly, the better reaction in the recovered animals could be due to the role played by the antigens of the piroplasmic form of the Theileria, in delayed hypersensitivity response