ABSTRACT
Squamous cell carcinoma [SCC] is the most frequent malignant tumor of oral cavity and esophagus. Ki67 antigen, a proliferative cell marker, has a significant role in determining biological behavior of the tumors. The purpose of this study was to determine the frequency of Ki67 expression in squamous cell carcinoma of oral cavity and esophagus and their correlated factors. In this descriptive study 40 paraffin embeded blocks of patients with oral and esophagus S.C.C [20 of each] were selected, clinicopathological data were recorded and Immunohistochemical staining [1HC] was done on the newly cut sections for Ki67 antigen. The immunostained cells were counted on 10 HPF [High Power Field] Percent of positive cell5 [Labeling Index] was calculated and then classified in to low [LI. 45%] and high [LI>45%] levels. Statistical analysis was performed in SPSS, version 13 and Chi-square test. The results showed that 24 cases [60%] were male and 16 cases [40%] were female. The mean age of the patients was 65.3 +/- 14.3. 45% of esophageal SCC specimens and 10% of oral SCC specimens were in high expression group. 55% of esophageal SCC and 90% of oral SCC were in low expression group and there was significant differences among oral and esophagus SCC [P<0.05]. Age and sex were not correlated with Ki67 expression but the grade and size of the tumors were significantly correlated with the expression of Ki67.[Oral: P=0.01, P=0.03 respectively], [Esophageal: P=0.02, P=0.03 respectively]. Ki67 expression in esophageal SCC was significantly higher than oral SCC which may define its more aggressive behavior. Age and sex were not correlated with Ki67 expression, but the grade and size of the tumors were significantly correlated with Ki67 expression
ABSTRACT
Metastatic tumors in the oral cavity account for about 1% of oral malignant tumors. They are mostly located in the mandible, predominantly in the molar region. All dentists and specialists must recognize oral metastatic lesions because they can be the first sign of an undiscovered malignancy, and also they can be easily mistaken with several different benign lesions. The aim of this study is to introduce a patient with metastatic follicular carcinoma of thyroid gland in the palate. A 71 years old female was referred to a private office with chief complaint of halitosis. A tumoral mass was found in the hard palate on clinical examination. Destruction of floor of maxillary sinus and nasal fossa, destruction of lateral wall of nasal fossa and tumor bulging in soft tissue of palate were seen on the CT scan. After seeing the pathology slides and conversation with the patient, previous medical history of thyroid surgery in The UK about 12 years ago was found. Folow up of patient revealed, she died 4months later by extensive lung metastasis
ABSTRACT
Finding an accurate and inexpensive method in measurement of bone width for determination of implant size is critical. Linear tomography is a cost effective method and there is no accurate finding about accuracy of linear tomography in evaluation of mandibular wide. The purpose of this study was to determine the accuracy of linear tomography in estimation of mandibular width. In this diagnostic study [estimation of one method], 23 sites of four dry mandibles were selected and marked at the crest with a metal ball markers. After linear tomography in these sites, the width of mandible in the superior border of inferior alveolar canal was measured. The mandibles were then sectioned. The Pearson's rank order correlation coefficient between linear tomography and real values was determined. Then with estimation of mean absolute differences by magnification factor, the percentages of errors in +/- 1mm error limits were determined. The regression equation was written for better determination of agreement rate between radiographic and real values. The percentages of errors in +/- 1 mm error limits were determined. The Pearson's rank order correlation coefficient between the linear tomography and real values was 0.813 [P<0.0001]. The mean absolute differences between the mandibular width in tomographic sections and real values was 0.3 mm [SD=1.13] and 56.5% of width measurements were within the +/- 1mm error limits. By the resulting linear regression equation 51.8% of tomographic values were located within the +/- 1mm error limits. Linear tomography in evaluation of mandibular width should be used more cautiously
ABSTRACT
Statement of Problem: Ameloblastomas are locally aggressive tumors with which the researchers have been mostly concerned to understand their biological behavior. Overexpression of minichromosome maintenance 3 [MCM3, a recently introduced marker] has been reported in different cancers. There is no study about MCM3 expression in ameloblastoma
Purpose: This study aims at the evaluation of MCM3, Ki-67 and p27 in ameloblastoma
Methods and Material: In this analytical study, 18 ameloblastomas were selected. Clinicohistopathological data were recorded and immuohistochemical staining was done on the newly cut sections for Ki-67 antigen, p27 kip1 and MCM3. The immunostained cells were counted on 10 HPF [Labeling Index] and then classified into negative [LI50] levels. Statistical analysis was performed in SPSS, version 13, using ordinal regression and Freidman tests. P value less than 0.05 was considered significant
Results: Among the lesions, 5 [27.8%] and 13 [72.2%] were negative and weak for Ki-67, respectively. Also, 5 [27.8%], 11[61.1%] and 2 [11.1%] lesions were negative, weak and moderate, respectively for p27. Ki-67 and p27 staining were seen more frequently in the ameloblast layer and stellate reticulum, respectively. None of the samples was stained by MCM3. There were significant differences among Ki-67, p27 and MCM3 expression in ameloblastomas but no differences were found between sex, age and tumor size and expression of these markers
Conclusion: Most of the ameloblastomas expressed low levels of Ki-67 and p27 [72.2% and 61.1%, respectively]. It could not be concluded exactly how these tumors will behave in the future, because low expression of p27 is in favor of cell proliferation [in contrast to low expression of Ki-67]. To find out how biological behavior of these tumors will be in the future, other markers should be evaluated and all the patients should be followed up. Regarding the negative expression of MCM3 in all lesions, it seems that MCM3 is not involved in the pathogenesis of ameloblastoma
ABSTRACT
Odontogenic keratocysts are usually in need of extensive treatment and are concerned mostly by researches in order to find their biological behavior. Minichromosome maintenances [MCM3] over-expression has been reported in several human beings affected by odontogenic. To our knowledge there is no article about MCM3 expression in odontogenic keratocyst. The aim of this study was to evaluate MCM3 in odontogenic keratocysts and compare it to Ki-67 and p27. Total of 23 OKC specimens were selected from Razi laboratory, they were reconfirmed by two indipendent pathologists. Clinicohistopathological data were recorded and standard streptavidin-biotin peroxidase method of immuohistochemical [IHC] staining was conducted for Ki-67 antigen, p27 Kipl and MCM3. The immunostained cells were counted on 10 HPF [Labeling Index] and then classified to negative, low, moderate and high levels. Statistical analysis was done by SPSS 13 using Freidman and Spearman's Rho tests and PLUM - Ordinal Regression. P-value less than 0.05 were considered significant. Ki-67 was stained mostly in the basal and suprabasal cells but p27 was found in the upper layers. No sample was stained by MCM3. There were significant differences among these proteins [MCM3 compared with Ki-67 and p27] not between p27 and Ki-67. No correlation was found among age, location and lesion size with these markers. Most of the odontogenic keratocysts expressed low levels of both Ki-67 and p27. We also found MCM3 is not implicated in the pathogenesis of OKC
Subject(s)
Humans , Immunohistochemistry , Ki-67 Antigen , BiomarkersABSTRACT
Abstract Introduction: Some of periapical lesions could be observed in a radiograph but the exact diagnosis is reach after microscopic evaluation. Unfortunately, in many cases periapical lesions are not diagnose because lack of a radiograph and then the dentist may pull the lesion out completely or incompletely. In this situation remains of the lesion can creat next problems
Objective: Determine frequency of periapical lesions among extracted teeth and classify them according to microscopic evaluation
Materials and Methods: This is a cross- sectional study. It was done in dental's offices and clinics in Rasht during 8 weeks, forty two lesions were collected among 5900 extracted teeth. The information was recorded and the lesions were sent within formalin10% to Pathology Laboratory in Poursina Hospital. The lesions were sliced by microtome, Stained [H and E], and then they were observed by a pathologist. The lesions were classified and statistical analysis was done by EPI calculator and SPSS 11.5
Results: 42 teeth [0.71%] had periapical lesions among 5900 extracted teeth. After microscopic evaluation the lesions were classified as periapical granuloma [57.14%], periapical cyst [33.33%] and other lesions [9.53%]. In this study the frequency of the lesions was similar in both sex, and patients often were between 3[rd] to 5[th] decades of their life. The must frequent location of the lesions in the jaw was in the posterior of maxilla, posterior of mandible, anterior of maxilla and anterior of mandible consequently
Conclusion: Almost there are seven periapical lesions among 1000 extracted teeth. Although the frequency of periapical lesions seems to be infrequent, all the periapical lesions should be sent to laboratory because lesions like mural ameloblastoma and traumatic bone cyst might be needed different treatment
ABSTRACT
Introduction: Beta-thalassemia patients require regular iron chelation therapy based on the severity of disease, treatment and its complications. Then these patients should receive regular medical care. The relation between dental caries and some diseases are identified. But unfortunately dental and oral problems in these patients received less attention and present information is little. Researchers also stated different opinions. The prevalence and severity of dental caries have been documented to be associated with a number of diseases. Little data are available on the association of dental caries with beta- thalassemia major. While some studies reported increase dental caries in these patients, other studies showed no differences between thalassemia patients and healthy controls
Objective: This study aimed to examine the levels of dental caries in beta-thalassemia patients and to compare it with matched nonthalassemic healthy controls
Materials and Methods: This study is cross sectional with control group. 60 thalassemia patients in health center of Iran Institute thalasemia and 60 healthy controls [referrals to college and patients healthy accompany] were assessed by DMF Index. The two groups were matched in age sex, socio-economic condition, oral hygiene, eating carious material and its frequency
Results: Mean DMF were 7.3 [SD=3.80] and 7.26 [SD=3.73] in patients and healthy controls respectively. There was no significant difference between patients and healthy controls in DMF index. Mean D and F were 5.55 and 1.33 in patients and controls respectively. There were significant differences between two groups in D and F indexes. There was not significant difference between DMF index in males and females in both groups
Conclusion: There was no significant difference in the two groups regarding DMF and it seems that there is no relation between Beta - thalasemia major and dental caries. As advanced treatment has greatly increased the probability for a thalassemic child to reach adult age, regular dental visits are recommended. Since decay was seen in patients more than controls, it's necessary to examine and fill them