ABSTRACT
The laboratory mouse is recognized as the pre-eminent model for genetic research. Awareness of chromosomal patterns of experimental animals increases their value for a variety of different fields of study. We aimed to study mitotic chromosome preparations from NIH, C57BL/6 and Razi strains of mice, which are outbred, inbred and partially inbred laboratory mice respectively. Bone marrow cells were prepared from 36 male and female mice, 12 from each strain, and stained by use of Giemsa staining and G-banding methods. Karyotyping of the samples showed that there was no difference in chromosomal numbers among the three mice strains, also the metaphase preparations of their diploid cells contained 40 chromosomes [2n = 40] and all chromosomes were telocentric. However, some differences in band tonality and the size of chromosomes were seen
ABSTRACT
Razi mouse is originally established from native Iranian mice at Razi institute and is susceptible to spontaneous mammary tumor. In this study, incidence, age and predisposing factors of tumor and some of biological characteristics of this strain were studied. There were significant differences in incidence of mammary tumor in Razi virgin females as compared with normally breeding and hormone treated breeding females in p < 0.05 but was the same with virgin NMRI females foster-nursed on Razi dams. However incidence in virgin Razi females foster-nursed on NMRI dams was very lesser than three mentioned groups. All mammary tumor tissues were histopathologically diagnosed adenocarcinoma. No significant differences were found in the levels of biological factors studied in tumor bearing and nonbearing Razi mice [p < 0.05]. Our results showed that milk factor may play the most important role in compare with genetic background and hormonal stimulation for development of mammary tumor in Razi mouse. Razi strain may represent a unique model to study of mammary tumor etiology and anticancer therapy
Subject(s)
Animals , Female , Breast Neoplasms/therapy , Mice , BreedingABSTRACT
Artificial insemination [AI] is a selective method which has been used in commercial rabbitries and for research purposes. The results of pregnancy rates were observed here which were comparable with natural mating. This experiment is conducted to provide the potentials of AI in the breeding center and also to compare this technique with on going natural mating method. One hundred sexually matured Dutch does were randomly divided into two equal groups. Does from experimental group were inseminated with diluted fresh semen and single dose of 0.2 ml of GnRH analog [Receptal] to each/doe was immediately injected intramuscularly. Does of control group were naturally mated with mature Dutch bucks. The environmental conditions were same in the both groups. The results of study showed that the 62% of does in control group had developed pregnancy where as in AI group 60% were pregnant. This result indicates the conception rate is similar in both groups and differences are not statistically significant
Subject(s)
Animals, Laboratory , Pregnancy , Rabbits , FertilizationABSTRACT
Blood glucose, serum bilirubin, serum enzymes, i.e. SAP, SGPT and SGOT, sodium and potassium, LH, FSH and estradiol level were determined in 93 women [aged 25-45]. These subjects were grouped according to the time of receiving intragluteal injections [200 rug NETEN 8-weeks intervals]. The groups of women receiving injections for 19-24 months showed a significant decrease [P < 0.01] in LH, FSH and estradiol levels. The pregnancy rate was 2%. Levels of LH, FSH and estradiol were elevated in women receiving injections for 25-38 M months. The pregnancy rate was 3%. The values were not significantly different when compared with controls. A significant increase in serum bilirubin, SAP, SGPT, SGOT, sodium and potassium levels were recorded in all treated groups. The control and treated groups did not show any significant difference with respect to blood glucose levels