Bioinformatics analysis and functional prediction of potential lung cancer associated genes in female non-smokers / 中国肿瘤生物治疗杂志

@#[Abstract] Objective: To explore the pathogenosis and prognostic markers for non-smoking female lung cancer patients with bioinfor‐ matics analysis and functional prediction of potential lung cancer associated genes in female non-smokers. Methods: Data for nonsmoking female patients with lung cancer were downloaded from the Gene Expression Omnibus (GEO) database and the differentially expressed genes (DEGs) were identified using GEO2R. DAVID online data base was used to perform gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG), and STRING online software was used to perform protein-protein interaction (PPI) analysis; then the plug-in (M-CODE) was used to screen the key DEGs; finally, GEPIA and Kaplan-Meier plotter were used to perform function prediction and prognosis analysis of key DEGs. Results: A total of 160 DEGs were screened, including 54 up-regulated and 106 down-regulated genes; GO enrichment analysis showed that these DEGs were mainly related to neovascularization, single cell adhesion, positive regulation of GTPase activity and signal transduction (all P<0.05). KEGG pathway analysis revealed that DEGs were mainly involved in cell adhesion molecules (CAMs), leukocyte transendothelial migration, tight junction and endocytosis (all P<0.05); PPI network analysis revealed 8 key DEGs, including TIE1, PECAM1, CLDN5, VEGFD, ICAM2, ESAM, EMCN and ROBO4. Conclusion: TIE1, CLDN5, ICAM2, ESAM, VEGFD and ROBO4 may be the research targets of the pathogenesis of non-smoking female lung cancer patients. PECAM1 and EMCN may be the new bio-markers to predict the progression and prognosis of nonsmoking female lung cancer patients.

Abnormal expression of b10 cell frequencies: possible relation to pathogenesis and disease severity of aplastic anemia

SUMMARY OBJECTIVE: Aplastic anemia (AA) is an immune-mediated disease that destroys hematopoietic cells through activated T lymphocytes. B lymphocyte-mediated humoral immunity also plays an important role in the pathogenesis of AA. Regulatory B cell (Breg) subpopulation, which is defined as "B10", secretes interleukin 10 (IL-10). The objective of our experiment was to investigate whether the scale-down proportion of B10 cells in AA patients may play a key role in the pathogenesis. METHODS: A total of 38 AA patients (14 SAA patients and 24 NSAA patients) and 20 healthy control subjects were included. All subjects did not suffer from autoimmune diseases or any other diseases affecting the immune system, such as infectious diseases. Bone marrow mononuclear cells (PBMCs) were isolated and analyzed by Flow cytometry (FCM) and Immunofluorescence double-labeling assay. The relationship between the relative proportions of B10 and ProB10 and their associations to AA, as well as disease severity, were assessed by common clinical indicators and then examined. RESULTS: Our analyses revealed AA patients had significantly lower proportions of peripheral B10 and B10pro compared to healthy controls. SAA patients had a substantially lower percentage of B10 cells and B10pro cells compared to NSAA patients. In addition, B10 cells and B10pro cells were negatively correlated with absolute neutrophil counts, hemoglobin levels and platelet, and absolute reticulocyte counts in AA patients. CONCLUSIONS: The present study attempted to elucidate the potential role of the scale-down proportion of B10 cells in the pathogenesis of AA.

RESUMO OBJETIVO: A anemia aplástica (AA) é uma doença imunomediada que destrói células hematopoiéticas por meio dos linfócitos T ativados. A imunidade humoral mediada por linfócitos B também desempenha um papel importante na patogênese da AA. A subpopulação de células B reguladoras (Breg), que é definida como "B10", secreta interleucina 10 (IL-10). No experimento, investigou-se se a proporção reduzida de células B10 nos pacientes de AA pode desempenhar um papel-chave na patogênese. MÉTODOS: Um total de 38 pacientes de AA (14 pacientes de anemia aplástica grave e 24 pacientes de anemia aplástica não grave) e 20 indivíduos de controle saudáveis foram incluídos. Todos os indivíduos não sofriam de doenças autoimunes ou de quaisquer outras doenças que afetam o sistema imunológico, tais como doenças contagiosas. As células mononucleares da medula óssea (PBMCs) eram isoladas e analisadas por citometria de fluxo (FCM) e ensaio de dupla marcação por imunofluorescência. A relação entre as proporções relativas de células B10 e as células ProB10 e as suas associações à AA, assim como a gravidade da doença avaliada por indicadores clínicos comuns, foram examinadas. RESULTADOS: Nossas análises revelaram que os pacientes de AA têm proporções significativamente menores de células B10 e células ProB10 periféricas em comparação com indivíduos de controle saudáveis. Os pacientes de anemia aplástica grave tiveram uma percentagem substancialmente menor de células B10 e células B10pro em comparação com pacientes de anemia aplástica não grave. Além disso, as células B10 e B10pro foram negativamente correlacionadas com contagens absolutas de neutrófilos, níveis de hemoglobina e plaquetas e contagem de reticulócitos absolutos nos pacientes de AA. CONCLUSÕES: Além disso, o estudo presente tentou elucidar o papel imunorregulatório potencial das células B10 na patogênese da AA e fornecer uma nova estratégia para a aplicação de imunoterapia baseada na célula B para tratar a AA no futuro.

Clinical effects of triamcinolone acetonide combined with laser photocoagulation on patients with retinal vein occlusion / 国际眼科杂志(Guoji Yanke Zazhi)

AlM: To observe the effects of the coelomic cavity injection of triamcinolone acetonide ( TA) combined with laser photocoagulation on patients with retinal vein occlusion. METHODS:Fifty-six patients of retinal obstruction with macular edema were accepted from January 2010 to December 2012 in our hospital. All patients received iodized lecithin and Xueshuantong. And, patients with central retinal vein occlusion ( CRVO ) , hemi- central retinal vein occlusion ( hemi-CRVO ) and branch retinal vein occlusion ( BRVO ) treated by TA combined with laser photocoagulation, respectively. Follow-up period was of at least 6mo RESULTS: After the treatment of 1, 3 and 6mo, the central foveal thickness was reduced significantly ( P CONCLUSlON:Basing on the traditional treatment, TA combined with laser photocoagulation is more effective in the treatment of retinal vein occlusion and is worthy of clinical usage.

Expression, purification and identification of St. Louis encephalitis virus-like particles in eukaryotic cells / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To express St. Louis encephalitis virus-like particles in mammalian cells, it will provide a prerequisite for further immunological diagnostic studies.</p><p><b>METHODS</b>293T-cell were transiently transfected with recombinant PreM-E plasmid. Expression and antigenicity of the purified protein were determined by transmission electron microscope (TEM), Western-Blot, immunofluorescence assay and ELISA.</p><p><b>RESULTS</b>Recombinant subviral particles, about 50 nm in diameter, were observed by TEM in the supernatant of transfected cells. The results of Western-Blot, IFA and ELISA showed the recombinant proteins retained immunoreactivity similar to those of native virus proteins.</p><p><b>CONCLUSION</b>St. Louis encephalitis virus-like particles has good antigenicity and physical appearance. It will provide a prerequisite for further immune diagnostic reagent.</p>

The development of quorum sensing phenomenon in pathogenic fungi / 第二军医大学学报

The quorum sensing commonly exists in procaryote kingdom, regulating various biologic functions. Recently similar phenomenon was also found in fungi world, which affecting both biofilm formation and dimorphism. In this article, we focus on the recent progress on quorum sensing of pathogenic fungi and discuss the possibility of taking quorum sensing molecule as a potential target for antifungl therapy.

Killer peptide-loaded nanoparticles: Characterization and evaluation of anticryptcoccal efficacy / 第二军医大学学报

Objective: To prepare killer peptide-loaded PLGA nanoparticles which have better effect in treating systemic crypotococcosis. Methods: We adopted a modified double-emulsion method to prepare the killer peptide-loaded PLGA noparticles, and the nanoparticles were characterized by using TEM, SEM, Dynamic Laser Scattering, drug loading analysis, ecapsulation efficacy assessment and in vitro release test. We also evaluated the antifungal activities of killer peptide and killer peptide-loaded PLGA nanoparticles against Cry ptOcoccus neoformans ATCC32609 by plate count method. In the in vivo study, the anticryptococcal efficacies of killer peptide and killer peptide-loaded PLGA nanoparticles were compared by observing the survival and colony-forming in vital organs in a systemic murine cryptococcosis model. Results1 We successfully synthesized killer peptide-loaded PLGA nanoparticles. In vitro anticryptococcal test showed that the killer peptide and killer peptide-loaded PLGA nanoparticles had significantly stronger anticryptococcal effect compared with the normal saline group (P0.05). In vits test demonstrated that mice treated with killer peptide-loaded PLGA nanoparticles (3 mg/kg or 5 mg/kg) had longer survival period and less fungal burden in vital organs than mice treated with free killer peptide (P0.05). Conclusion: The killer peptide-loaded PLGA nanoparticles are more effective in treating systemic murine cryptococcosis.

Analysis of the correlation between autofluorescence and central vision at advanced stage of the classical retinitis pigmentosa / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate autofluorescence (AF) images of classical retinitis pigmentosa (RP) at advanced stage correlated with central vision. METHODS: Thirty-seven patients (70 eyes) with classical RP at advanced stage were observed. AF imaging was performed using a Heidelberg Retina Angiograph 2 ( HRA-2) scanning laser system with the field 30°, the pixels 1 536×1 536 and pupil diameters >6mm. Some patients underwent automated static peri-metry and the flash electroretinogram(FERG) were performed according to International Society for Clinical Electrophysiology of Vision( ISCEV) standard. RESULTS: All patients were classified as four patterns according to their manifestation of AF they were intact, patchy, solitary island and central hypofluorescence patterns. Different pattern showed different central vision. The data demonstrate high correlation between the central cone function and AF changes of the fundus. There is significant correlation (n= 57, r= 0.385,P＜0.01) between central vision and the AF area and between age and AF area (n= 57, r= -2.67,P＜0.05).CONCLUSION: AF images is valuable to the monitoring of central cone function and in predicting retention of central vision. The changes of AF images correlated with central vision and different pattern showed different vision.